High level soluble expression, purification, and characterization of human ciliary neuronotrophic factor in Escherichia coli by single protein production system

Protein Expression and Purification

Volumn 96, Issue , 2014, Pages 8-13

  Wang, Ke ^a   Zhou, Fanfan ^b   Zhu, Lan ^a   Zhu, Xue ^a   Zhang, Kai ^a   Zhu, Ling ^c  

^a BEIJING HOSPITAL   (China)

^b UNIVERSITY OF SYDNEY   (Australia)

^c UNIVERSITY OF SYDNEY   (Australia)

Author keywords

Codon optimization; Escherichia coli; rhCNTF; Single protein production system

Indexed keywords

CILIARY NEUROTROPHIC FACTOR;

BIOSYNTHESIS; CELL PROTECTION; ESCHERICHIA COLI; GENE EXPRESSION; GENE THERAPY; GENETICS; HUMAN; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NEURODEGENERATIVE DISEASES; NUCLEOTIDE SEQUENCE; PLASMID;

BASE SEQUENCE; CILIARY NEUROTROPHIC FACTOR; CLONING, MOLECULAR; CYTOPROTECTION; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC THERAPY; HUMANS; MOLECULAR SEQUENCE DATA; NEURODEGENERATIVE DISEASES; PLASMIDS;

EID: 84894044162     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2014.01.008     Document Type: Article

References (24)

1. S.D. Skaper The neurotrophin family of neurotrophic factors: an overview Methods Mol. Biol. 846 2012 1 12
2. L. Aron, and R. Klein Repairing the parkinsonian brain with neurotrophic factors Trends Neurosci. 34 2011 88 100
3. V. Seydewitz, A. Rothermel, S. Fuhrmann, A. Schneider, W.J. DeGrip, and P.G. Layer et al. Expression of CNTF receptor-α in chick violet-sensitive cones with unique morphologic properties Invest. Ophthalmol. Vis. Sci. 45 2004 655 661 (Pubitemid 38140527)
4. U. Ernsberger, M. Sendtner, and H. Rohrer Proliferation and differentiation of embryonic chick sympathetic neurons: effects of ciliary neurotrophic factor Neuron 2 1989 1275 1284
5. R. Linker, R. Gold, and F. Luhder Function of neurotrophic factors beyond the nervous system: inflammation and autoimmune demyelination Crit. Rev. Immunol. 29 2009 43 68
6. P. Bongioanni, C. Reali, and V. Sogos Ciliary neurotrophic factor (CNTF) for amyotrophic lateral sclerosis or motor neuron disease, Cochrane DB Syst. Rev. 3 2004 CD004302
7. J. Bloch, A. Bachoud-Levi, N. Deglon, J. Lefaucheur, L. Winkel, and S. Palfi et al. Neuroprotective gene therapy for Huntington's disease, using polymer-encapsulated cells engineered to secrete human ciliary neurotrophic factor: results of a phase I study Hum. Gene Ther. 15 2004 968 975 (Pubitemid 39372573)
8. M.J. Pellegrino, and B.A. Habecker STAT3 integrates cytokine and neurotrophin signals to promote sympathetic axon regeneration Mol. Cell. Neurosci. 56 2013 272 282
9. R.S. Donovan, C. Robinson, and B. Glick Review: optimizing inducer and culture conditions for expression of foreign proteins under the control of the lac promoter J. Ind. Microbiol. 16 1996 145 154
10. I. Hunt From gene to protein: a review of new and enabling technologies for multi-parallel protein expression Protein Expr. Purif. 40 2005 1 22 (Pubitemid 40240966)
11. H.P. Sørensen, and K.K. Mortensen Advanced genetic strategies for recombinant protein expression in Escherichia coli J. Biotechnol. 115 2005 113 128 (Pubitemid 39656438)
12. R. Chen Bacterial expression systems for recombinant protein production: E. coli and beyond Biotechnol. Adv. 30 2012 1102 1107
13. A. Negro, G. Corona, E. Bigon, I. Martini, C. Grandi, and S. Skaper et al. Synthesis, purification, and characterization of human ciliary neuronotrophic factor from E. coli J. Neurosci. Res. 29 1991 251 260
14. J.R. McDonald, C. Ko, D. Mismer, D.J. Smith, and F. Collins Expression and characterization of recombinant human ciliary neurotrophic factor from Escherichia coli BBA-Gene Struct. Expr. 1090 1991 70 80
15. I. Cognet, F. Guilhot, S. Chevalier, A. Guay-Giroux, A. Bert, and G. Elson et al. Expression of biologically active mouse ciliary neutrophic factor (CNTF) and soluble CNTFRalpha in Escherichia coli and characterization of their functional specificities Eur. Cytokine Netw. 15 2004 255 262 (Pubitemid 39389922)
16. N.A. Burgess-Brown, S. Sharma, F. Sobott, C. Loenarz, U. Oppermann, and O. Gileadi Codon optimization can improve expression of human genes in Escherichia coli: a multi-gene study Protein Expr. Purif. 59 2008 94 102
17. H.G. Menzella Comparison of two codon optimization strategies to enhance recombinant protein production in Escherichia coli Microb. Cell Fact. 10 2011 15
18. M. Suzuki, L. Mao, and M. Inouye Single protein production (SPP) system in Escherichia coli Nat. Protoc. 2 2007 1802 1810
19. X. Zhu, K. Wang, K. Zhang, B. Huang, J. Zhang, and Y. Zhang et al. Ziyuglycoside II inhibits the growth of human breast carcinoma MDA-MB-435 cells via cell cycle arrest and induction of apoptosis through the mitochondria dependent pathway Int. J. Mol. Sci. 14 2013 18041 18055
20. J.E. Noble, and M.J. Bailey Quantitation of protein Methods Enzymol. 463 2009 73 95
21. N. Kaur, A.L. Wohlhueter, and S.W. Halvorsen Activation and inactivation of signal transducers and activators of transcription by ciliary neurotrophic factor in neuroblastoma cells Cell. Signal. 14 2002 419 429 (Pubitemid 34214846)
22. J. van Meerloo, G.J. Kaspers, and J. Cloos Cell sensitivity assays: the MTT assay 2011 Springer Cancer Cell Culture pp. 237-245
23. B.T. Selvaraj, and M. Sendtner CNTF, STAT3 and new therapies for axonal degeneration: what are they and what can they do? Expert Rev. Neurother. 13 2013 239 241
24. H. Sørensen, and K. Mortensen Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli Microb. Cell Fact. 4 2005 1