Methods for two-dimensional cell confinement

Methods in Cell Biology

Volumn 121, Issue , 2014, Pages 213-229

  Le Berre, Maël ^a   Zlotek Zlotkiewicz, Ewa ^a   Bonazzi, Daria ^a   Lautenschlaeger, Franziska ^a   Piel, Matthieu ^a  

^a CNRS   (France)

Author keywords

Biophysics; Cell adhesion; Cell mechanics; Cell migration; Gel assay; Microenvironment; Microfabrication

Indexed keywords

ACRYLIC ACID RESIN; BAYSILON; DIMETICONE; POLYACRYLAMIDE GELS;

AIR AND AIR RELATED PHENOMENA; ARTICLE; BIOPHYSICS; CELL ADHESION; CELL CULTURE; CELL MECHANICS; CELL MIGRATION; CELL MOTION; CHEMISTRY; CULTURE TECHNIQUE; EQUIPMENT; GEL ASSAY; HUMAN; MECHANICAL STRESS; METHODOLOGY; MICROENVIRONMENT; MICROSCOPY; MICROTECHNOLOGY;

BIOPHYSICS; CELL ADHESION; CELL MECHANICS; CELL MIGRATION; GEL ASSAY; MICROENVIRONMENT; MICROFABRICATION;

ACRYLIC RESINS; CELL ADHESION; CELL CULTURE TECHNIQUES; CELL MOVEMENT; CELLS, CULTURED; CONFINED SPACES; DIMETHYLPOLYSILOXANES; HUMANS; MICROSCOPY; STRESS, MECHANICAL;

EID: 84894025221     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-800281-0.00014-2     Document Type: Chapter

References (10)

1. Azioune A., Carpi N., Tseng Q., Théry M., Piel M. Protein micropatterns: A direct printing protocol using deep UVs. Methods in Cell Biology 2010, 97:133-146.
2. Barnhart E.L., Lee K.C., Keren K., Mogilner A., Theriot J.A. An adhesion-dependent switch between mechanisms that determine motile cell shape. PLoS Biology 2011, 9(5):e1001059.
3. Boudou T., Ohayon J., Picart C., Tracqui P. An extended relationship for the characterization of Young's modulus and Poisson's ratio of tunable polyacrylamide gels. Biorheology 2006, 43:721-728.
4. del Campo A., Greiner C. SU-8: A photoresist for high-aspect-ratio and 3D submicron lithography. Journal of Micromechanical Microengineering 2007, 17:R81-R95.
5. Justice B.A., Badr N.A., Felder R.A. 3D cell culture opens new dimensions in cell-based assays. Drug Discovery Today 2009, 14(1-2):102-107.
6. Lancaster O.M., Le Berre M., Dimitracopoulos A., Bonazzi D., Zlotek-Zlotkiewicz E., Picone R., et al. Mitotic rounding alters cell geometry to ensure efficient bipolar spindle formation. Developmental Cell 2013, 25(3):270-283.
7. Le Berre M., Aubertin J., Piel M. Fine control of nuclear confinement identifies a threshold deformation leading to lamina rupture and induction of specific genes. Integrative Biology 2012, 4(11):1406-1414.
8. Le Berre M., Liu Y.-J., Hu J., Maiuri P., Benichou O., Voituriez R., et al. Geometric friction directs cell migration 2013, unpublished.
9. Mitrossilis D., Fouchard J., Pereira D., Postic F., Richert A., Saint-Jean M., et al. Real-time single-cell response to stiffness. Proceedings of the National Academy of Sciences of the United States of America 2010, 107(38):16518-16523.
10. Velve-Casquillas G., Le Berre M., Piel M., Tran P.T. Microfluidic tools for cell biological research. Nano Today 2010, 5(1):28-47.