High activity and stability of codon-optimized phosphoenolpyruvate carboxylase from Photobacterium profundum SS9 at low temperatures and its application for in vitro production of oxaloacetate

Bioprocess and Biosystems Engineering

Volumn 37, Issue 2, 2014, Pages 331-335

  Park, Soohyun ^a   Hong, Soohye ^a   Pack, Seung Pil ^b   Lee, Jinwon ^a  

^a Sogang University   (South Korea)

^b Korea University   (South Korea)

Author keywords

Activity; Low temperature; Oxaloacetate; Phosphoenolpyruvate carboxylase; Stability

Indexed keywords

BASE SEQUENCE; CODON; COLD TEMPERATURE; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; OXALOACETATES; PHOSPHOENOLPYRUVATE CARBOXYLASE; PHOTOBACTERIUM;

EID: 84893869373     PISSN: 16157591     EISSN: 16157605     Source Type: Journal    
DOI: 10.1007/s00449-013-0981-8     Document Type: Article

References (13)

1. Robert CW, Gabor M (1972) Phosphoenolpyruvate carboxylase of Escherichia coli. J Biol Chem 247:5785-5792
2. Kwon YD, Kwon OH, Lee SH, Kim P (2007) The effect of NADP-dependent malic enzyme expression and anaerobic C4 metabolism in Escherichia coli compared with other anaplerotic enzymes. J Appl Microbiol 103:2340-2345 (Pubitemid 350191180)
3. Kwon YD, Lee SY, Kim P (2008) A physiology study of Escherichia coli overexpressing phosphoenolpyruvate carboxykinase. Biosci Biotechnol Biochem 72(4):1138-1141 (Pubitemid 351679382)
4. Park SH, Chang KS, Jin ES, Pack SP, Lee JW (2013) Oxaloacetate and malate production in engineered Escherichia coli by expression of codon-optimized phosphoenolpyruvate carboxylase2 gene from Dunaliella salina. Bioprocess Biosyst Eng 36:127-131
5. Kohji U, Tatsuo S (1976) Purification and characterization of phosphoenolpyruvate carboxylase from maize leaves. Plant Physiol 57:906-910
6. Jean R, William CP, David HT (1998) Purification and characterization of high- and low-molecular-mass isoforms of phosphoenolpyruvate carboxylase from Chlamydomonas reinhardtii. J Biochem 331:201-209
7. 2 sequestration application. Bioprocess Biosyst Eng 36:375-381
8. Vezzi A, Campanaro S, Angelo MD, Simonato F, Vitulo N, Lauro FM, Cestaro A, Malacrida G, Simionati B, Cannata N, Romualdi C, Bartlett DH, Valle G (2005) Life at Depth: Photobacterium profundum Genome Sequence and Expression Analysis. Science 307:1459-1460 (Pubitemid 40321941)
9. Yuichi N, Noriaki M, Chiaki K (1998) Photobacterium profundum sp. nov., a new, moderately barophilic bacterial species isolated from a deep-sea sediment. Extremophiles 2:1-7 (Pubitemid 28467965)
10. Park SH, Pack SP, Lee JW (2012) Expression of Codon-Optmized Phosphoenolpyruvate Carboxylase Gene from Glaciecola sp. HTCC2999 in Escherichia coli and its Application for C4 Chemical Production. Appl Biochem Biotechnol 167:1845-1853
11. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, New York
12. Dan W, Qiang L, Wangliang L, Jianmin X, Zhiguo S (2009) Improvement of succinate production by overexpression of a cyanobacterial carbonic anhydrase in Escherichia coli. Enzym Microbial Technol 45:491-497
13. Kodaki T, Katagiri F, Asano M, Izui K, Katsuki H (1985) Cloning of Phosphoenolpyruvate Carboxylase Gene from a Cyanobacterium Anacystis nidulans in Escherichia coli. J Biochem 97:533-539