Accessory factors promote AlfA-dependent plasmid segregation by regulating filament nucleation, disassembly, and bundling

Proceedings of the National Academy of Sciences of the United States of America

Volumn 111, Issue 6, 2014, Pages 2176-2181

  Polka, Jessica K ^a   Kollman, Justin M ^b   Mullins, R Dyche ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b MCGILL UNIVERSITY   (Canada)

Author keywords

Bacillus subtilis; Bacterial cytoskeleton; DNA segregation; Reconstitution

Indexed keywords

BACILLUS SUBTILIS; BACTERIAL CYTOSKELETON; DNA SEGREGATION; RECONSTITUTION;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; CENTROMERE; DNA, BACTERIAL; OPERON; PLASMIDS; PROTEIN CONFORMATION;

EID: 84893848615     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1304127111     Document Type: Article

References (30)

1. Jones LJ, Carballido-López R, Errington J (2001) Control of cell shape in bacteria: Helical, actin-like filaments in Bacillus subtilis. Cell 104(6):913-922.
2. Derman AI, et al. (2009) Phylogenetic analysis identifies many uncharacterized actinlike proteins (Alps) in bacteria: Regulated polymerization, dynamic instability and treadmilling in Alp7A. Mol Microbiol 73(4):534-552.
3. Garner EC, et al. (2011) Coupled, circumferential motions of the cell wall synthesis machinery and MreB filaments in B. subtilis. Science 333(6039):222-225.
4. Domínguez-Escobar J, et al. (2011) Processive movement of MreB-associated cell wall biosynthetic complexes in bacteria. Science 333(6039):225-228.
5. van Teeffelen S, et al. (2011) The bacterial actin MreB rotates, and rotation depends on cell-wall assembly. Proc Natl Acad Sci USA 108(38):15822-15827.
6. Komeili A, Li Z, Newman DK, Jensen GJ (2006) Magnetosomes are cell membrane invaginations organized by the actin-like protein MamK. Science 311(5758):242-245.
7. Ma X, Ehrhardt DW, Margolin W (1996) Colocalization of cell division proteins FtsZ and FtsA to cytoskeletal structures in living Escherichia coli cells by using green fluorescent protein. Proc Natl Acad Sci USA 93(23):12998-13003.
8. Møller-Jensen J, Jensen RB, Löwe J, Gerdes K (2002) Prokaryotic DNA segregation by an actin-like filament. EMBO J 21(12):3119-3127.
9. Garner EC, Campbell CS, Weibel DB, Mullins RD (2007) Reconstitution of DNA segregation driven by assembly of a prokaryotic actin homolog. Science 315(5816):1270-1274.
10. Gayathri P, et al. (2012) A bipolar spindle of antiparallel ParM filaments drives bacterial plasmid segregation. Science 338(6112):1334-1337.
11. Becker E, et al. (2006) DNA segregation by the bacterial actin AlfA during Bacillus subtilis growth and development. EMBO J 25(24):5919-5931.
12. Polka JK, Kollman JM, Agard DA, Mullins RD (2009) The structure and assembly dynamics of plasmid actin AlfA imply a novel mechanism of DNA segregation. J Bacteriol 191(20):6219-6230.
13. Popp D, et al. (2010) Polymeric structures and dynamic properties of the bacterial actin AlfA. J Mol Biol 397(4):1031-1041.
14. Fuesler JA, Li H-J (2012) Dynamic instability-A common denominator in prokaryotic and eukaryotic DNA segregation and cell division. Cell Mol Biol Lett 17(4):542-548.
15. Tanaka T, Ogura M (1998) A novel Bacillus natto plasmid pLS32 capable of replication in Bacillus subtilis. FEBS Lett 422(2):243-246.
16. Campbell CS, Mullins RD (2007) In vivo visualization of type II plasmid segregation: bacterial actin filaments pushing plasmids. J Cell Biol 179(5):1059-1066.
17. Jaqaman K, et al. (2008) Robust single-particle tracking in live-cell time-lapse sequences. Nat Methods 5(8):695-702.
18. Weber SC, Spakowitz AJ, Theriot JA (2010) Bacterial chromosomal loci move subdiffusively through a viscoelastic cytoplasm. Phys Rev Lett 104(23):238102.
19. Tanaka T (2010) Functional analysis of the stability determinant AlfB of pBET131, a miniplasmid derivative of bacillus subtilis (natto) plasmid pLS32. J Bacteriol 192(5): 1221-1230.
20. Flyvbjerg H, Jobs E, Leibler S (1996) Kinetics of self-assembling microtubules: An "inverse problem" in biochemistry. Proc Natl Acad Sci USA 93(12):5975-5979.
21. Theriot JA (2013) Why are bacteria different from eukaryotes? BMC Biol 11:119-135.
22. Rivera CR, Kollman JM, Polka JK, Agard DA, Mullins RD (2011) Architecture and assembly of a divergent member of the ParM family of bacterial actin-like proteins. J Biol Chem 286(16):14282-14290.
23. Fisher AJ, Rosenstiel TN, Shirk MC, Fall R (2001) Nonradioactive assay for cellular dimethylallyl diphosphate. Anal Biochem 292(2):272-279.
24. McCabe BC, Gollnick P (2004) Cellular levels of trp RNA-binding attenuation protein in Bacillus subtilis. J Bacteriol 186(15):5157-5159.
25. Edelstein A, Amodaj N, Hoover K, Vale R, Stuurman N (2001) Current Protocols in Molecular Biology (John Wiley & Sons, New York).
26. van den Ent F, Löwe J (2006) RF cloning: A restriction-free method for inserting target genes into plasmids. J Biochem Biophys Methods 67(1):67-74.
27. Stuurman N, Edelstein AD, Amodaj N, Hoover KH, Vale RD (2010) Computer control of microscopes using μManager. Curr Protoc Mol Biol 92(Suppl):14.20.2-14.20.17.
28. Rygus T, Scheler A, Allmansberger R, Hillen W (1991) Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus megaterium encoded regulon for xylose utilization. Arch Microbiol 155(6):535-542.
29. Vagner V, Dervyn E, Ehrlich SD (1998) A vector for systematic gene inactivation in Bacillus subtilis. Microbiology 144(Pt 11):3097-3104.
30. Ingerman E, Hsiao JY, Mullins RD (2013) Arp2/3 complex ATP hydrolysis promotes lamellipodial actin network disassembly but is dispensable for assembly. J Cell Biol 200(5):619-633.