A small GTPase-like protein fragment of Mycoplasma promotes tumor cell migration and proliferation in vitro via interaction with Rac1 and Stat3

Molecular Medicine Reports

Volumn 9, Issue 1, 2014, Pages 173-179

  Hu, Xiaopeng ^a   Yu, Jie ^b   Zhou, Xiang ^c   Li, Zhaoming ^d   Xia, Yun ^a   Luo, Zhiyong ^a   Wu, Yaqun ^a  

^a HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b First People's Hospital of Jiujiang City   (China)

^c Dept of Laboratory   (China)

^d THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIVERSITY   (China)

Author keywords

Mycoplasma; Rac1; Stat3; Tumor cell

Indexed keywords

ACTIN CYTOSKELETON; AMINO ACID MOTIFS; BACTERIAL PROTEINS; CELL MOVEMENT; CELL PROLIFERATION; HELA CELLS; HUMANS; MUTATION; MYCOPLASMA; PHOSPHORYLATION; RAC1 GTP-BINDING PROTEIN; STAT3 TRANSCRIPTION FACTOR; VIRULENCE FACTORS;

EID: 84893847339     PISSN: 17912997     EISSN: 17913004     Source Type: Journal    
DOI: 10.3892/mmr.2013.1766     Document Type: Article

References (29)

1. Sinkovics JG: Molecular biology of oncogenic inflammatory processes. I. Non-oncogenic and oncogenic pathogens, intrinsic inflammatory reactions without pathogens, and microRNA/DNA interactions (Review). Int J Oncol 40: 305-349, 2012.
2. Zhang S, Tsai S and Lo SC: Alteration of gene expression profiles during mycoplasma-induced malignant cell transformation. BMC Cancer 6: 116, 2006.
3. Tsai S, Wear DJ, Shih JW and Lo SC: Mycoplasmas and oncogenesis: persistent infection and multistage malignant transformation. Proc Natl Acad Sci USA 92: 10197-10201, 1995.
4. Rottem S: Interaction of mycoplasmas with host cells. Physiol Rev 83: 417-432, 2003.
5. Choi SY, Lim JW, Shimizu T, Kuwano K, Kim JM and Kim H: Reactive oxygen species mediate Jak2/Stat3 activation and IL-8 expression in pulmonary epithelial cells stimulated with lipid-associated membrane proteins from Mycoplasma pneumoniae. Inflamm Res 61: 493-501, 2012.
6. Rawadi G, Zugaza JL, Lemercier B, et al.: Involvement of small GTPases in Mycoplasma fermentans membrane lipoproteins-mediated activation of macrophages. J Biol Chem 274: 30794-30798, 1999.
7. Takai Y, Sasaki T and Matozaki T: Small GTP-binding proteins. Physiol Rev 81: 153-208, 2001.
8. Simon AR, Vikis HG, Stewart S, Fanburg BL, Cochran BH and Guan KL: Regulation of STAT3 by direct binding to the Rac1 GTPase. Science 290: 144-147, 2000.
9. Gupta SC, Hevia D, Patchva S, Park B, Koh W and Aggarwal BB: Upsides and downsides of reactive oxygen species for cancer: the roles of reactive oxygen species in tumorigenesis, prevention, and therapy. Antioxid Redox Signal 16: 1295-1322, 2012.
10. Millonig G, Ganzleben I, Peccerella T, et al.: Sustained submicromolar H2O2 levels induce hepcidin via signal transducer and activator of transcription 3 (STAT3). J Biol Chem 287: 37472-37482, 2012.
11. Tonozuka Y, Minoshima Y, Bao YC, et al.: A GTPase-activating protein binds STAT3 and is required for IL-6-induced STAT3 activation and for differentiation of a leukemic cell line. Blood 104: 3550-3557, 2004.
12. Kawashima T, Bao YC, Nomura Y, et al.: Rac1 and a GTPase-activating protein, MgcRacGAP, are required for nuclear translocation of STAT transcription factors. J Cell Biol 175: 937-946, 2006.
13. Teng TS, Lin B, Manser E, Ng DC and Cao X: Stat3 promotes directional cell migration by regulating Rac1 activity via its activator betaPIX. J Cell Sci 122: 4150-4159, 2009.
14. Debidda M, Wang L, Zang H, Poli V and Zheng Y: A role of STAT3 in Rho GTPase-regulated cell migration and proliferation. J Biol Chem 280: 17275-17285, 2005.
15. Azare J, Leslie K, Al-Ahmadie H, et al.: Constitutively activated Stat3 induces tumorigenesis and enhances cell motility of prostate epithelial cells through integrin beta 6. Mol Cell Biol 27: 4444-4453, 2007.
16. Alto NM, Shao F, Lazar CS, et al.: Identification of a bacterial type III effector family with G protein mimicry functions. Cell 124: 133-145, 2006.
17. Bulgin R, Raymond B, Garnett JA, et al.: Bacterial guanine nucleotide exchange factors SopE-like and WxxxE effectors. Infect Immun 78: 1417-1425, 2010.
18. Jackson LK, Nawabi P, Hentea C, Roark EA and Haldar K: The Salmonella virulence protein SifA is a G protein antagonist. Proc Natl Acad Sci USA 105: 14141-14146, 2008.
19. Ham JH, Majerczak DR, Nomura K, et al.: Multiple activities of the plant pathogen type III effector proteins WtsE and AvrE require WxxxE motifs. Mol Plant Microbe Interact 22: 703-712, 2009.
20. Hagemann UB, Mason JM, Miller KM and Arndt KM: Selectional and mutational scope of peptides sequestering the Jun-Fos coiled-coil domain. J Mol Biol 381: 73-88, 2008.
21. Luo X, Li Z, Li X, et al.: hSav1 interacts with HAX1 and attenuates its anti-apoptotic effects in MCF-7 breast cancer cells. Int J Mol Med 28: 349-355, 2011.
22. Fay FS, Taneja KL, Shenoy S, Lifshitz L and Singer RH: Quantitative digital analysis of diffuse and concentrated nuclear distributions of nascent transcripts, SC35 and poly(A). Exp Cell Res 231: 27-37, 1997.
23. Hachet-Haas M, Converset N, Marchal O, et al.: FRET and colocalization analyzer-a method to validate measurements of sensitized emission FRET acquired by confocal microscopy and available as an ImageJ Plug-in. Microsc Res Tech 69: 941-956, 2006.
24. Nobes CD and Hall A: Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia. Cell 81: 53-62, 1995.
25. Faruqi TR, Gomez D, Bustelo XR, Bar-Sagi D and Reich NC: Rac1 mediates STAT3 activation by autocrine IL-6. Proc Natl Acad Sci USA 98: 9014-9019, 2001.
26. Albertinazzi C, Cattelino A and de Curtis I: Rac GTPases localize at sites of actin reorganization during dynamic remodeling of the cytoskeleton of normal embryonic fibroblasts. J Cell Sci 112: 3821-3831, 1999.
27. Hoang MV, Nagy JA and Senger DR: Active Rac1 improves pathologic VEGF neovessel architecture and reduces vascular leak: mechanistic similarities with angiopoietin-1. Blood 117: 1751-1760, 2011.
28. Migeotte I, Omelchenko T, Hall A and Anderson KV: Rac1-dependent collective cell migration is required for specification of the anterior-posterior body axis of the mouse. PLoS Biol 8: e1000442, 2010.
29. Sehgal PB: Paradigm shifts in the cell biology of STAT signaling. Semin Cell Dev Biol 19: 329-340, 2008.