Rac GTPases localize at sites of actin reorganization during dynamic remodeling of the cytoskeleton of normal embryonic fibroblasts

Journal of Cell Science

Volumn 112, Issue 21, 1999, Pages 3821-3831

  Albertinazzi, Chiara ^a   Cattelino, Anna ^a   De Curtis, Ivan ^a  

^a San Raffaele Scientific Institute   (Italy)

Author keywords

Actin cytoskeleton; Adhesion; GTP binding protein; Integrin; Stress fiber

Indexed keywords

ACTIN; ANTIBODY; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE TRIPHOSPHATASE; NUCLEOTIDE;

ANIMAL CELL; ARTICLE; CELL MEMBRANE; CELL SHAPE; CELL STRUCTURE; CELLULAR DISTRIBUTION; CHICK EMBRYO; CONTROLLED STUDY; CYTOSKELETON; EMBRYO; ENZYME LOCALIZATION; ENZYME SUBSTRATE COMPLEX; FIBROBLAST; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ACTINS; ANIMALS; CELL CULTURE TECHNIQUES; CHICK EMBRYO; CYTOSKELETON; FIBROBLASTS; RAC GTP-BINDING PROTEINS;

ANIMALIA; GALLUS GALLUS;

EID: 0032694203     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (22)

1. Adamson, P., Paterson, H. F. and Hall, A. (1992). Intracellular localization of the P21rho proteins. J. Cell Biol. 119, 617-627.
2. Albertinazzi, C., Gilardelli, D., Paris, S., Longhi, R. and de Curtis, I. (1998). Overexpression of a neural-specific Rho family GTPase, cRac1B, selectively induces enhanced neuritogenesis and neurite branching in primary neurons. J. Cell Biol. 142, 815-825.
3. Bobak, D., Moorman, J., Guanzon, A., Gilmer, L. and Hahn, C. (1997). Inactivation of the small GTPase Rho disrupts cellular attachment and induces adhesion-dependent and adhesion-independent apoptosis. Oncogene 15, 2179-2189.
4. Cattelino, A., Longhi, R. and de Curtis, I. (1995). Differential distribution of two cytoplasmic variants of the α6β1 integrin laminin receptor in the ventral plasma membrane of embryonic fibroblasts. J. Cell Sci. 108, 3067-3078.
5. Cattelino, A., Cairo S., Malanchini, B. and de Curtis, I. (1997). Preferential localization of tyrosine-phosphorylated paxillin to focal adhesions. Cell Adhes. Commun. 4, 457-467.
6. Cattelino, A., Albertinazzi, C., Bossi, M., Critchley, D. R. and de Curtis, I. (1999). A cell-free system to study regulation of focal adhesions and of the connected actin cytoskeleton. Mol. Biol. Cell 10, 373-391.
7. Didsbury, J., Weber, R. F., Bokoch, G. M., Evans, T. and Snyderman, R. (1989). Rac, a novel ras-related family of proteins that are botulinum toxin substrates. J. Biol. Chem. 264, 16378-16382.
8. Hall, A. (1994). Small GTP-binding proteins and the regulatio of the actin cytoskeleton. Annu. Rev. Cell Biol. 10, 31-54.
9. Haataja, L., Groffen, J. and Heisterkamp, N. (1997). Characterization of Rac3, a novel member of the Rho family. J. Biol. Chem. 272, 20384-20388.
10. Horwitz, A., Duggan, K., Greggs, R., Decker, C. and Buck, C. (1985). The cell substrate attachment (CSAT) antigen has properties of a receptor for laminin and fibronectin. J. Cell Biol. 101, 2134-2144.
11. Kozma, R., Ahmed, S., Best, A. and Lim L. (1995). The Ras-related protein Cdc42Hs and bradykinin promote formation of peripheral actin microspikes and filopodia in Swiss 3T3 fibroblasts. Mol. Cell. Biol. 15, 1942-1952.
12. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriaphage T4. Nature 277, 680-685.
13. Machesky, L. M. and Hall, A. (1997). Role of actin polymerization and adhesion to extracellular matrix in Rac-and Rho-induced cytoskeletal reorganization. J. Cell Biol. 138, 913-926.
14. Malosio, M. L., Gilardelli, D., Paris, S., Albertinazzi, C. and de Curtis, I. (1997). Differential expression of distinct members of the Rho family of GTP-binding proteins during neuronal development: Identification of Rac 1B, a new neural-specific member of the family. J. Neurosci. 17, 6717-6728.
15. McCallum, S. J., Erickson, J. W. and Cerione, R. A. (1998). Characterization of the association of the actin-binding protein, IQGAP, and activated Cdc42 with Golgi membranes. J. Biol. Chem. 273, 22537-22544.
16. Neff, N. T., Lowrey, C., Decker, C., Tovar, A., Damsky, C., Buck, C. and Horwitz, A. F. (1982). A monoclonal antibody detaches embryonic skeletal muscle from extracellular matrices. J. Cell Biol. 95, 654-666.
17. Nobes, C. D. and Hall, A. (1995). Rho, Rac, and Cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia. Cell 81, 53-62.
18. Ridley, A. J., Paterson, H. F., Johnston, C. L., Diekmann, D. and Hall, A. (1992). The small GTP-binding protein rac regulates growth factor-induced membrane ruffling. Cell 70, 401-410.
19. Ridley, A. J. (1996). Rho: theme and variations. Curr. Biol. 6, 1256-1264.
20. Robertson, D., Paterson, H. F., Adamson P., Hall, A. and Monaghan, P. (1995). Ultrastructural localization of ras-related proteins using epitope-tagged plasmids. J. Histochem. Cytochem. 43, 471-480.
21. Takaishi, K., Sasaki, T., Kameyama, T., Tsukita, S., Tsukita, S. and Takai, Y. (1995). Translocation of activated Rho from the cytoplasm to membrane ruffling area, cell-cell adhesion sites and cleavage furrows. Oncogene 11, 39-48.
22. Tomaselli, K. J., Damsky, C. H. and Reichardt, L. F. (1988). Purification and characterization of mammalian inlegrins expressed by rat neuronal cell line (PC12): evidence that they may function as α/β heterodimeric receptors for collagen IV and laminin. J. Cell Biol. 107, 1241-1252.