Evaluation and identification of hepatitis B virus entry inhibitors using HepG2 cells overexpressing a membrane transporter NTCP

Biochemical and Biophysical Research Communications

Volumn 443, Issue 3, 2014, Pages 808-813

  Iwamoto, Masashi ^a,b   Watashi, Koichi ^a,b   Tsukuda, Senko ^a,c   Aly, Hussein Hassan ^a   Fukasawa, Masayoshi ^a   Fujimoto, Akira ^a   Suzuki, Ryosuke ^a   Aizaki, Hideki ^a   Ito, Takayoshi ^d   Koiwai, Osamu ^b   Kusuhara, Hiroyuki ^e   Wakita, Takaji ^a  

^a NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

^b TOKYO UNIVERSITY OF SCIENCE   (Japan)

^c RIKEN   (Japan)

^d SHOWA UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^e UNIVERSITY OF TOKYO   (Japan)

Author keywords

Cyclosporin; DMSO; HBV; Infection; NTCP; Oxysterol

Indexed keywords

ANTIVIRUS AGENT; BROMSULFOPHTHALEIN; CHOLESTEROL DERIVATIVE; CHOLIC ACID; CYCLOSPORIN A; CYCLOSPORIN B; DEXTRAN SULFATE; DIMETHYL SULFOXIDE; HEPARIN; HEPATITIS B SURFACE ANTIBODY; HEPATITIS B SURFACE ANTIGEN; HEPATITIS B(E) ANTIGEN; MEMBRANE PROTEIN; NEUTRALIZING ANTIBODY; OXYSTEROL; PROGESTERONE; SODIUM BILE ACID COTRANSPORTER; TETRACYCLINE; URSODEOXYCHOLIC ACID; VIRUS DNA;

ARTICLE; CELL CLONE; CELL SURFACE; DISEASE PREDISPOSITION; DRUG IDENTIFICATION; DRUG SCREENING; GENE OVEREXPRESSION; GENOTYPE; HEPATITIS B; HEPATITIS B VIRUS; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; INOCULATION; LIVER CELL; PRIORITY JOURNAL; VIRUS ENTRY;

HEPATITIS B VIRUS; TUPAIA;

AB; ANTIBODY; CCCDNA; COVALENTLY CLOSED CIRCULAR DNA; CS; CYCLOSPORIN; DIMETHYL SULFOXIDE; DMSO; GENOME EQUIVALENT; GEQ; HBC; HBS; HBV; HBV CORE PROTEIN; HBV SURFACE PROTEIN; HEPATITIS B VIRUS; HYDROXYCHOLESTEROL; INFECTION; NTCP; OHC; OXYSTEROL; PHH; PRIMARY HUMAN HEPATOCYTES; PRIMARY TUPAIA HEPATOCYTES; PTH; SODIUM TAUROCHOLATE COTRANSPORTING POLYPEPTIDE;

ANIMALS; DIMETHYL SULFOXIDE; HEP G2 CELLS; HEPATITIS B; HEPATITIS B VIRUS; HUMANS; ORGANIC ANION TRANSPORTERS, SODIUM-DEPENDENT; RNA, MESSENGER; SYMPORTERS; TUPAIA; VIRUS INTERNALIZATION;

EID: 84893702742     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2013.12.052     Document Type: Article

References (22)

1. D. Grimm, R. Thimme, and H.E. Blum HBV life cycle and novel drug targets Hepatol. Int. 5 2011 644 653
2. J.M. Pawlotsky, G. Dusheiko, A. Hatzakis, D. Lau, G. Lau, T.J. Liang, S. Locarnini, P. Martin, D.D. Richman, and F. Zoulim Virologic monitoring of hepatitis B virus therapy in clinical trials and practice: recommendations for a standardized approach Gastroenterology 134 2008 405 415
3. M. Rapicetta, C. Ferrari, and M. Levrero Viral determinants and host immune responses in the pathogenesis of HBV infection J. Med. Virol. 67 2002 454 457
4. F. Zoulim Hepatitis B virus resistance to antiviral drugs: where are we going? Liver Int. 31 Suppl. 1 2011 111 116
5. P. Gripon, S. Rumin, S. Urban, J. Le Seyec, D. Glaise, I. Cannie, C. Guyomard, J. Lucas, C. Trepo, and C. Guguen-Guillouzo Infection of a human hepatoma cell line by hepatitis B virus Proc. Natl. Acad. Sci. USA 99 2002 15655 15660
6. D. Glebe, and S. Urban Viral and cellular determinants involved in hepadnaviral entry World J. Gastroenterol. 13 2007 22 38
7. J.M. Gottwein, and J. Bukh Cutting the gordian knot-development and biological relevance of hepatitis C virus cell culture systems Adv. Virus Res. 71 2008 51 133
8. H. Yan, G. Zhong, G. Xu, W. He, Z. Jing, Z. Gao, Y. Huang, Y. Qi, B. Peng, H. Wang, L. Fu, M. Song, P. Chen, W. Gao, B. Ren, Y. Sun, T. Cai, X. Feng, J. Sui, and W. Li Sodium taurocholate cotransporting polypeptide is a functional receptor for human hepatitis B and D virus Elife 1 2012 e00049
9. M.S. Anwer, and B. Stieger Sodium-dependent bile salt transporters of the SLC10A transporter family: more than solute transporters Pflugers Arch. 2013 (Epub ahead of print)
10. P.J. Meier, and B. Stieger Bile salt transporters Annu. Rev. Physiol. 64 2002 635 661
11. C. Seeger, and W.S. Mason Sodium-dependent taurocholic cotransporting polypeptide: a candidate receptor for human hepatitis B virus Gut 62 2013 1093 1095
12. R.B. Kim, B. Leake, M. Cvetkovic, M.M. Roden, J. Nadeau, A. Walubo, and G.R. Wilkinson Modulation by drugs of human hepatic sodium-dependent bile acid transporter (sodium taurocholate cotransporting polypeptide) activity J. Pharmacol. Exp. Ther. 291 1999 1204 1209
13. S.K. Ladner, M.J. Otto, C.S. Barker, K. Zaifert, G.H. Wang, J.T. Guo, C. Seeger, and R.W. King Inducible expression of human hepatitis B virus (HBV) in stably transfected hepatoblastoma cells: a novel system for screening potential inhibitors of HBV replication Antimicrob. Agents Chemother. 41 1997 1715 1720
14. K. Watashi, G. Liang, M. Iwamoto, H. Marusawa, N. Uchida, T. Daito, K. Kitamura, M. Muramatsu, H. Ohashi, T. Kiyohara, R. Suzuki, J. Li, S. Tong, Y. Tanaka, K. Murata, H. Aizaki, and T. Wakita Interleukin-1 and tumor necrosis factor-alpha trigger restriction of hepatitis B virus infection via a cytidine deaminase activation-induced cytidine deaminase (AID) J. Biol. Chem. 288 2013 31715 31727
15. S. Mita, H. Suzuki, H. Akita, H. Hayashi, R. Onuki, A.F. Hofmann, and Y. Sugiyama Inhibition of bile acid transport across Na+/taurocholate cotransporting polypeptide (SLC10A1) and bile salt export pump (ABCB 11)-coexpressing LLC-PK1 cells by cholestasis-inducing drugs Drug Metab. Dispos. 34 2006 1575 1581
16. A. Schulze, K. Mills, T.S. Weiss, and S. Urban Hepatocyte polarization is essential for the productive entry of the hepatitis B virus Hepatology 55 2012 373 383
17. M. Koyanagi, M. Hijikata, K. Watashi, O. Masui, and K. Shimotohno Centrosomal P4.1-associated protein is a new member of transcriptional coactivators for nuclear factor-kappaB J. Biol. Chem. 280 2005 12430 12437
18. P. Gripon, I. Cannie, and S. Urban Efficient inhibition of hepatitis B virus infection by acylated peptides derived from the large viral surface protein J. Virol. 79 2005 1613 1622
19. P. Gripon, C. Diot, A. Corlu, and C. Guguen-Guillouzo Regulation by dimethylsulfoxide, insulin, and corticosteroids of hepatitis B virus replication in a transfected human hepatoma cell line J. Med. Virol. 28 1989 193 199
20. K. Watashi, A. Sluder, T. Daito, S. Matsunaga, A. Ryo, S. Nagamori, M. Iwamoto, S. Nakajima, S. Tsukuda, K. Borroto-Esoda, M. Sugiyama, Y. Tanaka, Y. Kanai, H. Kusuhara, M. Mizokami, T. Wakita, Cyclosporin A and its analogs inhibit hepatitis B virus entry into cultured hepatocytes through targeting a membrane transporter NTCP, Hepatology, in press.
21. A. Schulze, P. Gripon, and S. Urban Hepatitis B virus infection initiates with a large surface protein-dependent binding to heparan sulfate proteoglycans Hepatology 46 2007 1759 1768
22. O. Hantz, R. Parent, D. Durantel, P. Gripon, C. Guguen-Guillouzo, and F. Zoulim Persistence of the hepatitis B virus covalently closed circular DNA in HepaRG human hepatocyte-like cells J. Gen. Virol. 90 2009 127 135