Human endothelial cell-based assay for endotoxin as sensitive as the conventional Limulus Amebocyte Lysate assay

Biomaterials

Volumn 35, Issue 10, 2014, Pages 3180-3187

  Unger, Ronald E ^a   Peters, Kirsten ^b   Sartoris, Anne ^a   Freese, Christian ^a   Kirkpatrick, C James ^a  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

^b UNIVERSITY OF ROSTOCK   (Germany)

Author keywords

Endothelial; Endotoxin; Limulus amebocyte assay; Lipopolysaccharide

Indexed keywords

CELL ADHESION MOLECULES; ENDOTHELIAL; ENDOTOXIN; HUMAN MICROVASCULAR ENDOTHELIAL CELLS; HUMAN UMBILICAL VEIN ENDOTHELIAL (HUVEC) CELLS; IMMUNOFLUORESCENT STAINING; LIMULUS AMEBOCYTE LYSATE; LIPOPOLYSACCHARIDES;

BIOLOGICAL MATERIALS; BIOMATERIALS; BIOMOLECULES;

ENDOTHELIAL CELLS;

BIOMATERIAL; CELL ADHESION MOLECULE; ENDOTHELIAL LEUKOCYTE ADHESION MOLECULE 1; ENDOTOXIN; ESCHERICHIA COLI LIPOPOLYSACCHARIDE; NANOPARTICLE;

ARTICLE; BACTERIUM CONTAMINATION; CELL ASSAY; CELL FUNCTION; CELL GROWTH; CELL TYPE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEEDBACK SYSTEM; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE TEST; IN VITRO STUDY; LIMULUS LYSATE TEST; MICROVASCULAR ENDOTHELIAL CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; SENSITIVITY ANALYSIS; SKIN; STAINING; UMBILICAL VEIN ENDOTHELIAL CELL;

BACTERIA (MICROORGANISMS); LIMULUS; MAMMALIA;

ENDOTHELIAL; ENDOTOXIN; LIMULUS AMEBOCYTE ASSAY; LIPOPOLYSACCHARIDE;

ANIMALS; CELLS, CULTURED; ENDOTHELIUM, VASCULAR; ENDOTOXINS; HORSESHOE CRABS; HUMANS; LIMIT OF DETECTION;

EID: 84893637188     PISSN: 01429612     EISSN: 18785905     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2013.12.059     Document Type: Article

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