Dynamic autofluorescence imaging of intracellular components inside living cells using direct electron beam excitation

Biomedical Optics Express

Volumn 5, Issue 2, 2014, Pages 378-386

  Nawa, Yasunori ^a,b   Inami, Wataru ^a,c   Miyake, Aki ^a,c   Ono, Atsushi ^a,c   Kawata, Yoshimasa ^a,c   Lin, Sheng ^a   Terakawa, Susumu ^c,d  

^a SHIZUOKA UNIVERSITY   (Japan)

^b JAPAN SOCIETY FOR THE PROMOTION OF SCIENCE   (Japan)

^c JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^d HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENCE;

AUTOFLUORESCENCE IMAGING; CELLULAR COMPONENTS; DYNAMIC OBSERVATION; ELECTRON BEAM EXCITATION; ENDOGENOUS SUBSTANCES; FLUORESCENT MATERIALS; FOCUSED ELECTRON BEAMS; INTRACELLULAR COMPONENTS;

ELECTRON BEAMS;

NANOPARTICLE;

ARTICLE; AUTOFLUORESCENCE IMAGING; CHEMICAL ANALYSIS; CURVE FITTING; ELECTRON BEAM; ELECTRON SCATTER; EPIFLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; PHOTOLUMINESCENCE; PHYSICAL PHENOMENA; SCANNING ELECTRON MICROSCOPY; SIGNAL NOISE RATIO;

EID: 84893523015     PISSN: None     EISSN: 21567085     Source Type: Journal    
DOI: 10.1364/BOE.5.000378     Document Type: Article

References (26)

1. D. L. Taylor and Y.-L. Wang, Methods in Cell Biology vol. 30 (Academic Press Inc., San Diego, 1989).
2. M. Zochowski, M. Wachowiak, C. X. Falk, L. B. Cohen, Y. W. Lam, S. Antic, and D. Zecevic, "Imaging membrane potential with voltage-sensitive dyes, " Biol. Bull. 198(1), 1-21 (2000).
3. R. A. Gottlieb, J. Nordberg, E. Skowronski, and B. M. Babior, "Apoptosis induced in Jurkat cells by several agents is preceded by intracellular acidification, " Proc. Natl. Acad. Sci. U.S.A. 93(2), 654-658 (1996).
4. J. P. Y. Kao, "Practical aspects of measuring [Ca2+] with fluorescent indicators, " in Methods in Cell Biology vol. 40, R. Nuccitelli, ed. (Academic Press, Inc., San Diego, 1994).
5. S. L. Sensi, D. Ton-That, P. G. Sullivan, E. A. Jonas, K. R. Gee, L. K. Kaczmarek, and J. H. Weiss, "Modulation of mitochondrial function by endogenous Zn2+pools, " Proc. Natl. Acad. Sci. U.S.A. 100(10), 6157-6162 (2003).
6. M. Monici, "Cell and tissue autofluorescence research and diagnostic applications, " Biotechnol. Annu. Rev. 11, 227-256 (2005).
7. M. De Mets, "Cathodoluminescence of organic chemicals, " in Principles and Techniques of Scanning Electron Microscopy vol. 2, M.A. Hayat, ed. (Van Nostrand Reinhold, NewYork, 1974).
8. G. Nichols, "Applications of cathodoluminescence spectroscopy and imaging in the characterisation of pharmaceutical materials, " Eur. J. Pharm. Sci. 45(1-2), 19-42 (2012).
9. P. J. Fisher, W. S. Wessels, A. B. Dietz, and F. G. Prendergast, "Enhanced biological cathodoluminescence, " Opt. Commun. 281(7), 1901-1908 (2008).
10. J. Niitsuma, H. Oikawa, E. Kimura, T. Ushiki, and T. Sekiguchi, "Cathodoluminescence investigation of organic materials, " J. Electron Microsc. (Tokyo) 54(4), 325-330 (2005).
11. N. de Jonge, N. Poirier-Demers, H. Demers, D. B. Peckys, and D. Drouin, "Nanometer-resolution electron microscopy through micrometers-thick water layers, " Ultramicroscopy 110(9), 1114-1119 (2010).
12. H. Nishiyama, M. Suga, T. Ogura, Y. Maruyama, M. Koizumi, K. Mio, S. Kitamura, and C. Sato, "Reprint of: Atmospheric scanning electron microscope observes cells and tissues in open medium through silicon nitride film, " J. Struct. Biol. 172(2), 191-202 (2010).
13. M. Suga, H. Nishiyama, Y. Konyuba, S. Iwamatsu, Y. Watanabe, C. Yoshiura, T. Ueda, and C. Sato, "The Atmospheric Scanning Electron Microscope with open sample space observes dynamic phenomena in liquid or gas, " Ultramicroscopy 111(12), 1650-1658 (2011).
14. O. Cohen, R. Beery, S. Levit, J. Ilany, I. Schwartz, M. Shabtai, D. Anaby, D. Cohen, R. Alfici, A. Czerniak, and A. Karasik, "Scanning electron microscopy of thyroid cells under fully hydrated conditions-a novel technique for a seasoned procedure: a brief observation, " Thyroid 16(10), 997-1001 (2006).
15. U. M. Mirsaidov, H. Zheng, Y. Casana, and P. Matsudaira, "Imaging protein structure in water at 2.7 nm resolution by transmission electron microscopy, " Biophys. J. 102(4), L15-L17 (2012).
16. Y. Nawa, W. Inami, A. Chiba, A. Ono, A. Miyakawa, Y. Kawata, S. Lin, and S. Terakawa, "Dynamic and highresolution live cell imaging by direct electron beam excitation, " Opt. Express 20(5), 5629-5635 (2012).
17. D. C. Joy, Monte Carlo Modeling for Electron Microscopy and Microanalysis (Oxford Univ. Press, New York, 1995).
18. W. Inami, K. Nakajima, A. Miyakawa, and Y. Kawata, "Electron beam excitation assisted optical microscope with ultra-high resolution, " Opt. Express 18(12), 12897-12902 (2010).
19. B. K. Meyer, H. Alves, D. M. Hofmann, W. Kriegseis, D. Forster, F. Bertram, J. Christen, A. Hoffmann, M. Straßburg, M. Dworzak, U. Haboeck, and A. V. Rodina, "Bound exciton and donor-acceptor pair recombinations in ZnO, " Phys. Status Solidi 241(2), 231-260 (2004) (b).
20. M. R. Phillips, O. Gelhausen, and E. M. Goldys, "Cathodoluminescence properties of zinc oxide nanoparticles, " Phys. Status Solidi 201(2), 229-234 (2004) (a).
21. Y. L. Wu, C. S. Lim, S. Fu, A. I. Y. Tok, H. M. Lau, F. Y. C. Boey, and X. T. Zeng, "Surface modifications of ZnO quantum dots for bio-imaging, " Nanotechnology 18(21), 215604 (2007).
22. A. Rose, Advances in Electronics vol.1 (Academic Press Inc. New York, 1948).
23. C. A. Schnaitman, "Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X-100, " J. Bacteriol. 108(1), 545-552 (1971).
24. D. B. Peckys and N. de Jonge, "Visualizing gold nanoparticle uptake in live cells with liquid scanning transmission electron microscopy, " Nano Lett. 11(4), 1733-1738 (2011).
25. T. Hürter, W. Bröcker, and K. A. Hossmann, "Evaluation of vasogenic edema in experimental brain tumors by cathodoluminescence and fluorescence microscopy, " Histochemistry 72(2), 249-254 (1981).
26. T. Nakano, T. Fujimoto, H. Koike, and K. Ogawa, "Application of analytical color fluorescence electron microscopy to biomedical field: I. vitamin A ester in rat retina, " Acta Historiem. Cytochem. 23(6), 753-767 (1990).