Screening fluorescent voltage indicators with spontaneously spiking HEK cells

PLoS ONE

Volumn 8, Issue 12, 2013, Pages

  Park, Jeehae ^a   Werley, Christopher A ^a   Venkatachalam, Veena ^a   Kralj, Joel M ^a   Dib Hajj, Sulayman D ^b   Waxman, Stephen G ^b   Cohen, Adam E ^a,c  

^a HARVARD UNIVERSITY   (United States)

^b VA CONNECTICUT HEALTHCARE SYSTEM   (United States)

^c HARVARD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTION POTENTIALS; ARCHAEAL PROTEINS; DNA PRIMERS; HEK293 CELLS; HUMANS; INDICATORS AND REAGENTS; MICROSCOPY, FLUORESCENCE; MUTAGENESIS; MUTATION; NAV1.3 VOLTAGE-GATED SODIUM CHANNEL; NEUROSCIENCES; POTASSIUM CHANNELS, INWARDLY RECTIFYING; SODIUM CHANNELS; VIDEO RECORDING; VOLTAGE-SENSITIVE DYE IMAGING;

EID: 84893505516     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0085221     Document Type: Article

References (25)

1. Peterka DS, Takahashi H, Yuste R (2011) Imaging voltage in neurons. Neuron 69: 9-21. doi:10.1016/j.neuron.2010.12.010. PubMed: 21220095.
2. Scanziani M, Häusser M (2009) Electrophysiology in the age of light. Nature 461: 930-939. doi:10.1038/nature08540. PubMed: 19829373.
3. Kralj JM, Hochbaum DR, Douglass AD, Cohen AE (2011) Electrical spiking in escherichia coli probed with a fluorescent voltage indicating protein. Science 333: 345-348. doi:10.1126/science.1204763. PubMed: 21764748.
4. Pucihar G, Kotnik T (2009) Measuring the induced membrane voltage with di-8-ANEPPS. J Vis Exp 33: e1659. PubMed: 19927116.
5. Opitz T, De Lima AD, Voigt T (2002) Spontaneous development of synchronous oscillatory activity during maturation of cortical networks in vitro. J Neurophysiol 88: 2196-2206. doi:10.1152/jn.00316.2002. PubMed: 12424261. (Pubitemid 35346025)
6. Ma J, Guo L, Fiene SJ, Anson BD, Thomson JA et al. (2011) High purity human-induced pluripotent stem cell-derived cardiomyocytes: Electrophysiological properties of action potentials and ionic currents. Am J Physiol Heart Circ Physiol 301: H2006-H2017. doi:10.1152/ajpheart.00694.2011. PubMed: 21890694.
7. Akerboom J, Chen TW, Wardill TJ, Tian L, Marvin JS et al. (2012) Optimization of a GCaMP calcium indicator for neural activity imaging. J Neurosci 32: 13819-13840. doi:10.1523/JNEUROSCI.2601-12.2012. PubMed: 23035093.
8. Kirkton RD, Bursac N (2011) Engineering biosynthetic excitable tissues from unexcitable cells for electrophysiological and cell therapy studies. Nat Commun 2: 300. doi:10.1038/ncomms1302. PubMed: 21556054.
9. Cummins TR, Aglieco F, Renganathan M, Herzog RI, Dib-Hajj SD et al. (2001) Nav1. 3 sodium channels: Rapid repriming and slow closed-state inactivation display quantitative differences after expression in a mammalian cell line and in spinal sensory neurons. J Neurosci 21: 5952-5961. PubMed: 11487618. (Pubitemid 32737728)
10. Hille B (2001) Ion channels of excitable membranes. Sunderland, MA: Sinauer.
11. Shah BS, Rush AM, Liu S, Tyrrell L, Black JA et al. (2004) Contactin associates with sodium channel Nav1. 3 in native tissues and increases channel density at the cell surface. J Neurosci 24: 7387-7399. doi:10.1523/JNEUROSCI. 0322-04.2004. PubMed: 15317864. (Pubitemid 39100707)
12. Butterweck A, Gergs U, Elfgang C, Willecke K, Traub O (1994) Immunochemical characterization of the gap junction protein connexin45 in mouse kidney and transfected human HeLa cells. J Membr Biol 141: 247-256. PubMed: 7807524. (Pubitemid 24305246)
13. Del Re AM, Woodward JJ (2005) Inhibition of gap junction currents by the abused solvent toluene. Drug Alcohol Depend 78: 221-224. doi:10.1016/j. drugalcdep.2004.10.005. PubMed: 15845326. (Pubitemid 40544610)
14. Miller EW, Lin JY, Frady EP, Steinbach PA, Kristan WB et al. (2012) Optically monitoring voltage in neurons by photo-induced electron transfer through molecular wires. Proc Natl Acad Sci U S A 109: 2114-2119. doi:10.1073/pnas.1120694109. PubMed: 22308458.
15. Davidenko JM, Pertsov AV, Salomonsz R, Baxter W, Jalife J (1992) Stationary and drifting spiral waves of excitation in isolated cardiac muscle. Nature 355: 349-351. doi:10.1038/355349a0. PubMed: 1731248.
16. Meron E (1992) Pattern formation in excitable media. Physics Reports 218: 1-66. doi:10.1016/0370-1573(92)90098-K.
17. Maclaurin DM, Venkatachalam VV, Lee H, Cohen AE (2013) Mechanism of voltage-sensitive fluorescence in a microbial rhodopsin. Proc Natl Acad Sci U S A 110: 5939-5944. doi:10.1073/pnas.1215595110. PubMed: 23530193.
18. Zhang J, Davidson RM, Wei MD, Loew LM (1998) Membrane electric properties by combined patch clamp and fluorescence ratio imaging in single neurons. Biophys J 74: 48-53. doi:10.1016/S0006-3495(98)77765-3. PubMed: 9449308. (Pubitemid 28041735)
19. Grinvald A, Hildesheim R, Farber IC, Anglister L (1982) Improved fluorescent probes for the measurement of rapid changes in membrane potential. Biophys J 39: 301-308. doi:10.1016/S0006-3495(82)84520-7. PubMed: 7139029. (Pubitemid 13240779)
20. Jin L, Han Z, Platisa J, Wooltorton JRA, Cohen LB et al. (2012) Single action potentials and subthreshold electrical events imaged in neurons with a fluorescent protein voltage probe. Neuron 75: 779-785. doi:10.1016/j.neuron. 2012.06.040. PubMed: 22958819.
21. Kralj JM, Douglass AD, Hochbaum DR, Maclaurin D, Cohen AE (2012) Optical recording of action potentials in mammalian neurons using a microbial rhodopsin. Nat Methods 9: 90-95. doi:10.1038/nchembio.1135. PubMed: 22120467.
22. Chanda B, Blunck R, Faria LC, Schweizer FE, Mody I et al. (2005) A hybrid approach to measuring electrical activity in genetically specified neurons. Nat Neurosci 8: 1619-1626. doi:10.1038/nn1558. PubMed: 16205716. (Pubitemid 41573678)
23. Park K, Deutsch Z, Li JJ, Oron D, Weiss S (2012) Single molecule quantum-confined stark effect measurements of semiconductor nanoparticles at room temperature. ACS Nano 6: 10013-10023. doi:10.1021/nn303719m. PubMed: 23075136.
24. Marshall JD, Schnitzer MJ (2013) Optical strategies for sensing neuronal voltage using quantum dots and other semiconductor nanocrystals. ACS Nano 7: 4601-4609. doi:10.1021/nn401410k. PubMed: 23614672.
25. Samad OA, Tan AM, Cheng X, Foster E, Dib-Hajj SD et al. (2013) Virus-mediated shRNA knockdown of Nav1. 3 in rat dorsal root ganglion attenuates nerve injury-induced neuropathic pain. Molecular Therapy 21: 49-56. PubMed: 22910296.