The 3' overhangs at Tetrahymena thermophila telomeres are packaged by four proteins, Pot1a, Tpt1, Pat1, and Pat2

Eukaryotic Cell

Volumn 13, Issue 2, 2014, Pages 240-245

  Premkumar, Vidjaya Letchoumy ^a   Cranert, Stacey ^a   Linger, Benjamin R ^a,d   Morin, Gregg B ^b,c   Minium, Sasha ^a   Price, Carolyn ^a  

^a UNIVERSITY OF CINCINNATI   (United States)

^b BRITISH COLUMBIA CANCER AGENCY   (Canada)

^c UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^d INDIANA WESLEYAN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTOZOAL DNA; PROTOZOAL PROTEIN; TELOMERE BINDING PROTEIN;

ARTICLE; DNA FLANKING REGION; GENETICS; METABOLISM; PROTEIN BINDING; TELOMERE; TETRAHYMENA THERMOPHILA;

3' FLANKING REGION; DNA, PROTOZOAN; PROTEIN BINDING; PROTOZOAN PROTEINS; TELOMERE; TELOMERE-BINDING PROTEINS; TETRAHYMENA THERMOPHILA;

EID: 84893421115     PISSN: 15359778     EISSN: None     Source Type: Journal    
DOI: 10.1128/EC.00275-13     Document Type: Article

References (42)

1. Murnane JP. 2012. Telomere dysfunction and chromosome instability. Mutat. Res. 730:28-36. http://dx.doi.org/10.1016/j.mrfmmm.2011.04.008.
2. O'Sullivan RJ, Karlseder J. 2010. Telomeres: protecting chromosomes against genome instability. Nat. Rev. Mol. Cell Biol. 11:171-181. http://dx.doi.org/10.1038/nrm2848.
3. Stewart JA, Chaiken MF, Wang F, Price CM. 2012. Maintaining the end: roles of telomere proteins in end-protection, telomere replication and length regulation. Mutat. Res. 730:12-19. http://dx.doi.org/10.1016/j.mrfmmm.2011.08.011.
4. Palm W, de Lange T. 2008. How shelterin protects mammalian telomeres. Annu. Rev. Genet. 42:301-334. http://dx.doi.org/10.1146/annurev.genet.41.110306.130350.
5. Dehé PM, Cooper JP. 2010. Fission yeast telomeres forecast the end of the crisis. FEBS Lett. 584:3725-3733. http://dx.doi.org/10.1016/j.febslet.2010.07.045.
6. Wellinger RJ, Zakian VA. 2012. Everything you ever wanted to know about Saccharomyces cerevisiae telomeres: beginning to end. Genetics 191: 1073-1105. http://dx.doi.org/10.1534/genetics.111.137851.
7. Giraud-Panis MJ, Teixeira MT, Geli V, Gilson E. 2010. CST meets shelterin to keep telomeres in check. Mol. Cell 39:665-676. http://dx.doi.org/10.1016/j.molcel.2010.08.024.
8. Denchi EL, de Lange T. 2007. Protection of telomeres through independent control of ATM and ATR by TRF2 and POT1. Nature 448:1068-1071. http://dx.doi.org/10.1038/nature06065.
9. Churikov D, Price CM. 2008. Pot1 and cell cycle progression cooperate in telomere length regulation. Nat. Struct. Mol. Biol. 15:79-84. http://dx.doi.org/10.1038/nsmb1331.
10. Ribeyre C, Shore D. 2012. Anticheckpoint pathways at telomeres in yeast. Nat. Struct. Mol. Biol. 19:307-313. http://dx.doi.org/10.1038/nsmb.2225.
11. Zaug AJ, Podell ER, Nandakumar J, Cech TR. 2010. Functional interaction between telomere protein TPP1 and telomerase. Genes Dev. 24: 613-622. http://dx.doi.org/10.1101/gad.1881810.
12. Churikov D, Corda Y, Luciano P, Geli V. 2013. Cdc13 at a crossroads of telomerase action. Front. Oncol. 3:39. http://dx.doi.org/10.3389/fonc.2013.000309.
13. Qi H, Zakian VA. 2000. The Saccharomyces telomere-binding protein Cdc13p interacts with both the catalytic subunit ofDNApolymerase alpha and the telomerase-associated Est1 protein. Genes Dev. 14:1777-1788. http://dx.doi.org/10.1101/gad.14.14.1777.
14. Zhao Y, Sfeir AJ, Zou Y, Buseman CM, Chow TT, Shay JW, Wright WE. 2009. Telomere extension occurs at most chromosome ends and is uncoupled from fill-in in human cancer cells. Cell 138:463-475. http://dx.doi.org/10.1016/j.cell.2009.05.026.
15. Wang F, Stewart JA, Kasbek C, Zhao Y, Wright WE, Price CM. 2012. Human CST has independent functions during telomere duplex replication and C-strand fill-in. Cell Rep. 2:1096-1103. http://dx.doi.org/10.1016/j.celrep.2012.10.007.
16. Nandakumar J, Bell CF, Weidenfeld I, Zaug AJ, Leinwand LA, Cech TR. 2012. The TEL patch of telomere protein TPP1 mediates telomerase recruitment and processivity. Nature 492:285-289. http://dx.doi.org/10.1038/nature11648.
17. Latrick CM, Cech TR. 2010. POT1-TPP1 enhances telomerase processivity by slowing primer dissociation and aiding translocation. EMBO J. 29:924-933. http://dx.doi.org/10.1038/emboj.2009.409.
18. Wang F, Podell ER, Zaug AJ, Yang Y, Baciu P, Cech TR, Lei M. 2007. The POT1-TPP1 telomere complex is a telomerase processivity factor. Nature 445:506-510. http://dx.doi.org/10.1038/nature05454.
19. Evans SK, Lundblad V. 1999. Est1 and Cdc13 as comediators of telomerase access. Science 286:117-120. http://dx.doi.org/10.1126/science.286.5437.117.
20. Taggart AK, Teng SC, Zakian VA. 2002. Est1p as a cell cycle-regulated activator of telomere-bound telomerase. Science 297:1023-1026. http: //dx.doi.org/10.1126/science.1074968.
21. Puglisi A, Bianchi A, Lemmens L, Damay P, Shore D. 2008. Distinct roles for yeast Stn1 in telomere capping and telomerase inhibition. EMBO J. 27:2328-2339. http://dx.doi.org/10.1038/emboj.2008.158.
22. Jahn CL, Klobutcher LA. 2002. Genome remodeling in ciliated protozoa. Annu. Rev. Microbiol. 56:489-520. http://dx.doi.org/10.1146/annurev.micro.56.012302.160916.
23. Eisen JA, Coyne RS, Wu M, Wu D, Thiagarajan M, Wortman JR, Badger JH, Ren Q, Amedeo P, Jones KM, Tallon LJ, Delcher AL, Salzberg SL, Silva JC, Haas BJ, Majoros WH, Farzad M, Carlton JM, Smith RK, Jr, Garg J, Pearlman RE, Karrer KM, Sun L, Manning G, Elde NC, Turkewitz AP, Asai DJ, Wilkes DE, Wang Y, Cai H, Collins K, Stewart BA, Lee SR, Wilamowska K, Weinberg Z, Ruzzo WL, Wloga D, Gaertig J, Frankel J, Tsao CC, Gorovsky MA, Keeling PJ, Waller RF, Patron NJ, Cherry JM, Stover NA, Krieger CJ, del Toro C, Ryder HF, Williamson SC, Barbeau RA, Hamilton EP, Orias E. 2006. Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a model eukaryote. PLoS Biol. 4:e286. http://dx.doi.org/10.1371/journal.pbio.0040286.
24. Turkewitz AP, Orias E, Kapler G. 2002. Functional genomics: the coming of age for Tetrahymena thermophila. Trends Genet. 18:35-40. http: //dx.doi.org/10.1016/S0168-9525(01)02560-4.
25. Zeng Z, Min B, Huang J, Hong K, Yang Y, Collins K, Lei M. 2011. Structural basis for Tetrahymena telomerase processivity factor Teb1 binding to single-stranded telomeric-repeat DNA. Proc. Natl. Acad. Sci. U. S. A. 108:20357-20361. http://dx.doi.org/10.1073/pnas.1113624108.
26. Hong K, Upton H, Miracco EJ, Jiang J, Zhou ZH, Feigon J, Collins K. 2013. Tetrahymena telomerase holoenzyme assembly, activation, and inhibition by domains of the p50 central hub. Mol. Cell. Biol. 33:3962-3971. http://dx.doi.org/10.1128/MCB.00792-13.
27. Jacobs SA, Podell ER, Cech TR. 2006. Crystal structure of the essential N-terminal domain of telomerase reverse transcriptase. Nat. Struct. Mol. Biol. 13:218-225. http://dx.doi.org/10.1038/nsmb1054.
28. Jiang J, Miracco EJ, Hong K, Eckert B, Chan H, Cash DD, Min B, Zhou ZH, Collins K, Feigon J. 2013. The architecture of Tetrahymena telomerase holoenzyme. Nature 496:187-192. http://dx.doi.org/10.1038 /nature12062.
29. Berman AJ, Gooding AR, Cech TR. 2010. Tetrahymena telomerase protein p65 induces conformational changes throughout telomerase RNA (TER) and rescues telomerase reverse transcriptase and TER assembly mutants. Mol. Cell. Biol. 30:4965-4976. http://dx.doi.org/10.1128/MCB.00827-10.
30. Karrer KM. 1999. Tetrahymena genetics: two nuclei are better than one. Methods Cell Biol. 62:127-186. http://dx.doi.org/10.1016/S0091-679X(08)61529-0.
31. Kirk KE, Blackburn EH. 1995. An unusual sequence arrangement in the telomeres of the germ-line micronucleus in Tetrahymena thermophila. Genes Dev. 9:59-71. http://dx.doi.org/10.1101/gad.9.1.59.
32. Kapler GM. 1993. Developmentally regulated processing and replication of the Tetrahymena rDNA minichromosome. Curr. Opin. Genet. Dev. 3:730-735. http://dx.doi.org/10.1016/S0959-437X(05)80091-7.
33. Jacob NK, Skopp R, Price CM. 2001. G-overhang dynamics at Tetrahymena telomeres. EMBO J. 20:4299-4308. http://dx.doi.org/10.1093 /emboj/20.15.4299.
34. Cohen P, Blackburn EH. 1998. Two types of telomeric chromatin in Tetrahymena thermophila. J. Mol. Biol. 280:327-344. http://dx.doi.org/10.1006/jmbi.1998.1867.
35. Jacob NK, Lescasse R, Linger BR, Price CM. 2007. Tetrahymena POT1a regulates telomere length and prevents activation of a cell cycle checkpoint. Mol. Cell. Biol. 27:1592-1601. http://dx.doi.org/10.1128/MCB.01975-06.
36. Linger BR, Morin GB, Price CM. 2011. The Pot1a-associated proteins Tpt1 and Pat1 coordinate telomere protection and length regulation in Tetrahymena. Mol. Biol. Cell 22:4161-4170. http://dx.doi.org/10.1091 /mbc.E11-06-0551.
37. Shang Y, Song X, Bowen J, Corstanje R, Gao Y, Gaertig J, Gorovsky MA. 2002. A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila. Proc. Natl. Acad. Sci. U. S. A. 99:3734-3739. http://dx.doi.org/10.1073/pnas.052016199.
38. Mead CL, Kuzyk MA, Moradian A, Wilson GM, Holt RA, Morin GB. 2010. Cytosolic protein interactions of the schizophrenia susceptibility gene dysbindin. J. Neurochem. 113:1491-1503. http://dx.doi.org/10.1111 /j.1471-4159.2010.06690.x.
39. Nelson JD, Denisenko O, Sova P, Bomsztyk K. 2006. Fast chromatin immunoprecipitation assay. Nucleic Acids Res. 34:e2. http://dx.doi.org /10.1093/nar/gnj004.
40. Jacob NK, Stout AR, Price CM. 2004. Modulation of telomere length dynamics by the subtelomeric region of Tetrahymena telomeres. Mol. Biol. Cell 15:3719-3728. http://dx.doi.org/10.1091/mbc.E04-03-0237.
41. Min B, Collins K. 2009. An RPA-related sequence-specific DNA-binding subunit of telomerase holoenzyme is required for elongation processivity and telomere maintenance. Mol. Cell 36:609-619. http://dx.doi.org/10.1016/j.molcel.2009.09.041.
42. Min B, Collins K. 2010. Multiple mechanisms for elongation processivity within the reconstituted Tetrahymena telomerase holoenzyme. J. Biol. Chem. 285:16434-16443. http://dx.doi.org/10.1074/jbc.M110.119172.