DNA sampling from body swabs of terrestrial slugs (Gastropoda: Pulmonata): A simple and non-invasive method for molecular genetics approaches

Journal of Molluscan Studies

Volumn 80, Issue 1, 2014, Pages 99-101

  Morinha, Francisco ^a,b   Travassos, Paulo ^b   Carvalho, Diogo ^b   Magalhães, Paula ^c   Cabral, João A ^b   Bastos, Estela ^a  

^a UNIVERSITY OF TRÁS OS MONTES AND ALTO DOURO   (Portugal)

^b UNIVERSITY OF TRÁS OS MONTES AND ALTO DOURO   (Portugal)

^c UNIVERSITY OF PORTO   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

GENETIC ANALYSIS; MOLECULAR ANALYSIS; MORPHOLOGY; SLUG;

EID: 84893042836     PISSN: 02601230     EISSN: 14643766     Source Type: Journal    
DOI: 10.1093/mollus/eyt045     Document Type: Article

References (21)

1. ARMBRUSTER, G.F.J., KOLLER, B. & BAUR, B. 2005. Foot mucus and periostracum fraction as non-destructive source of DNA in the land snail Arianta arbustorum, and the development of new microsatellite loci. Conservation Genetics, 6: 313-316.
2. BARKER, G.M. 2001. The biology of terrestrial molluscs. CABI Publishing, Wallingford, UK.
3. BARKER, G.M. 2002. Molluscs as crop pests. CABI Publishing,Wallingford, UK.
4. BICKLEY, J. & HOPKINS, D. 1999. Inhibitors and enhancers of PCR. In: Analytical molecular biology: quality and validation (G.C. Saunders & H.C. Parkes, eds), pp. 81-102. Royal Society of Chemistry, Cambridge, UK.
5. CAMERON, R.A.D., POKRYSZKO, B.M. & MARTINS, A.M.F. 2012. Land snail faunas on Santa Maria (Azores): local diversity in an old, isolated and disturbed island. Journal of Molluscan Studies, 78: 268-274.
6. FOLMER, O., BLACK, M., HOEH, W., LUTZ, R. & VRIJENHOEK, R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3: 294-299.
7. HANSEN, T.O., SIMONSEN, M.K., NIELSEN, F.C. & HUNDRUP, Y.A. 2007. Collection of blood, saliva, and buccal cell samples in a pilot study on the Danish nurse cohort: comparison of the response rate and quality of genomic sample. Cancer Epidemiology, Biomarkers & Prevention, 16: 2072-2076.
8. KAPPES, H. 2006. Relations between forest management and slug assemblages (Gastropoda) of deciduous regrowth forests. Forest Ecology and Management, 237: 450-457.
9. KAWAI, K., SHIMIZU, M., HUGHES, R.N. & TAKENAKA, O. 2004. A non-invasive technique for obtaining DNA from marine intertidal snails. Journal of the Marine Biological Association of the United Kingdom, 84: 773-774.
10. PALMER, A.N.S., STYAN, C.A. & SHEARMAN, D.C.A. 2008. Foot mucus is a good source for non-destructive genetic sampling in Polyplacophora. Conservation Genetics, 9: 229-231.
11. PEREIRA, J.C., CHAVES, R., BASTOS, E., LEITAO, A. & GUEDES-PINTO, H. 2011. An efficient method for genomic DNA extraction from different molluscs species. International Journal of Molecular Sciences, 12: 8086-8095.
12. PINEAU, P., HENRY, M., SUSPE NE, R., MARCHIO, A., DETTAI, A., DEBRUYNE, R., PETIT, T., LÉ CU, A., MOISSON, P., DEJEAN, A., WAIN-HOBSON, S. & VARTANIAN, J.-P. 2005. A universal primer set for PCR amplification of nuclear histone H4 genes from all animal species. Molecular Biology and Evolution, 22: 582-588.
13. PLATTS, E.A. & SPEIGHT, M.C.D. 1988. The taxonomy and distribution of the Kerry slug Geomalacus maculosus Allman, 1843 (Mollusca: Arionidae) with a discussion of its status as a threatened species. Irish Naturalists' Journal, 22: 417-430.
14. QUINTEIRO, J., RODRI GUEZ-CASTRO, J., CASTILLEJO, J., IGLESIAS-PINEIRO, J. & REY-ME NDEZ, M. 2005. Phylogeny of slug species of the genus Arion: evidence of monophyly of Iberian endemics and of the existence of relict species in Pyrenean refuges. Journal of Zoological Systematics and Evolutionary Research, 43: 139-148.
15. RÉGNIER, C., GARGOMINY, O., FALKNER, G. & PUILLANDRE, N. 2011. Foot mucus stored on FTA cards is a reliable and noninvasive source of DNA for genetics studies in molluscs. Conservation Genetics Resources, 3: 377-382.
16. SEVERNS, P.M. 2005. Response of terrestrial mollusc community to an autumn prescribed burn in rare wetland prairie of western Oregon, USA. Journal of Molluscan Studies, 71: 181-187.
17. SMITH, A.M. 2010. Gastropod secretory glands and adhesive gels. In: Biological adhesive systems. From nature to technical and medical application (J. Von Byern & I. Grunwald, eds), pp. 41-51. Springer, Wien, New York
18. SOKOLOV, E.P. 2000. An improved method for DNA isolation from mucopolysaccharide-rich molluscan tissues. Journal of Molluscan Studies, 66: 573-575.
19. VAN DER MEIJ, S.E.T., MOOLENBEEK, R.G. & HOEKSEMA, B.W. 2009. Decline of the Jakarta Bay molluscan fauna linked to human impact. Marine Pollution Bulletin, 59: 101-107.
20. WADE, C.M., MORDAN, P.B. & NAGGS, F. 2006. Evolutionary relationships among the Pulmonate land snails and slugs (Pulmonata, Stylommatophora). Biological Journal of the Linnean Society, 87: 593-610.
21. WINNEPENNINCKX, B., BACKELJAU, T. & DE WACHTER, R. 1993. Extraction of high molecular weight DNA from molluscs. Trends in Genetics, 9: 407.