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Volumn 13, Issue 1, 2014, Pages

A dedicated microarray for in-depth analysis of pre-mRNA splicing events: Application to the study of genes involved in the response to targeted anticancer therapies

Author keywords

DNA chip; Pre mRNA splicing; SRSF2; Targeted anticancer therapies

Indexed keywords

GEFITINIB; MESSENGER RNA; MESSENGER RNA PRECURSOR; PROTEIN; SRSF2 PROTEIN; UNCLASSIFIED DRUG;

EID: 84892900538     PISSN: None     EISSN: 14764598     Source Type: Journal    
DOI: 10.1186/1476-4598-13-9     Document Type: Article
Times cited : (8)

References (48)
  • 2
    • 75849145292 scopus 로고    scopus 로고
    • Expansion of the eukaryotic proteome by alternative splicing
    • 10.1038/nature08909, 3443858, 20110989
    • Nilsen TW, Graveley BR. Expansion of the eukaryotic proteome by alternative splicing. Nature 2010, 463:457-463. 10.1038/nature08909, 3443858, 20110989.
    • (2010) Nature , vol.463 , pp. 457-463
    • Nilsen, T.W.1    Graveley, B.R.2
  • 4
    • 76149130971 scopus 로고    scopus 로고
    • Alternative splicing: global insights
    • 10.1111/j.1742-4658.2009.07521.x, 20082635
    • Hallegger M, Llorian M, Smith CWJ. Alternative splicing: global insights. FEBS J 2010, 277:856-866. 10.1111/j.1742-4658.2009.07521.x, 20082635.
    • (2010) FEBS J , vol.277 , pp. 856-866
    • Hallegger, M.1    Llorian, M.2    Smith, C.W.J.3
  • 5
    • 78049416081 scopus 로고    scopus 로고
    • Alternative pre-mRNA splicing regulation in cancer: pathways and programs unhinged
    • 10.1101/gad.1973010, 2964746, 21041405
    • David CJ, Manley JL. Alternative pre-mRNA splicing regulation in cancer: pathways and programs unhinged. Genes Dev 2010, 24:2343-2364. 10.1101/gad.1973010, 2964746, 21041405.
    • (2010) Genes Dev , vol.24 , pp. 2343-2364
    • David, C.J.1    Manley, J.L.2
  • 6
    • 4644299291 scopus 로고    scopus 로고
    • Alternative splicing of Bcl-2-related genes: functional consequences and potential therapeutic applications
    • Akgul C, Moulding DA, Edwards SW. Alternative splicing of Bcl-2-related genes: functional consequences and potential therapeutic applications. Cell Mol Life Sci 2004, 61:2189-2199.
    • (2004) Cell Mol Life Sci , vol.61 , pp. 2189-2199
    • Akgul, C.1    Moulding, D.A.2    Edwards, S.W.3
  • 7
    • 84872455821 scopus 로고    scopus 로고
    • Splice variants in apoptotic pathway
    • Miura K, Fujibuchi W, Unno M. Splice variants in apoptotic pathway. Exp Oncol 2012, 34:212-217.
    • (2012) Exp Oncol , vol.34 , pp. 212-217
    • Miura, K.1    Fujibuchi, W.2    Unno, M.3
  • 8
    • 21244439380 scopus 로고    scopus 로고
    • Regulation of apoptosis by alternative pre-mRNA splicing
    • 10.1016/j.molcel.2005.05.026, 15989960
    • Schwerk C, Schulze-Osthoff K. Regulation of apoptosis by alternative pre-mRNA splicing. Mol Cell 2005, 19:1-13. 10.1016/j.molcel.2005.05.026, 15989960.
    • (2005) Mol Cell , vol.19 , pp. 1-13
    • Schwerk, C.1    Schulze-Osthoff, K.2
  • 9
    • 0742323558 scopus 로고    scopus 로고
    • Nonsense-mediated mRNA decay: splicing, translation and mRNP dynamics
    • 10.1038/nrm1310, 15040442
    • Maquat LE. Nonsense-mediated mRNA decay: splicing, translation and mRNP dynamics. Nat Rev Mol Cell Biol 2004, 5:89-99. 10.1038/nrm1310, 15040442.
    • (2004) Nat Rev Mol Cell Biol , vol.5 , pp. 89-99
    • Maquat, L.E.1
  • 10
    • 49949105213 scopus 로고    scopus 로고
    • The multiple lives of NMD factors: balancing roles in gene and genome regulation
    • 10.1038/nrg2402, 3711694, 18679436
    • Isken O, Maquat LE. The multiple lives of NMD factors: balancing roles in gene and genome regulation. Nat Rev Genet 2008, 9:699-712. 10.1038/nrg2402, 3711694, 18679436.
    • (2008) Nat Rev Genet , vol.9 , pp. 699-712
    • Isken, O.1    Maquat, L.E.2
  • 11
    • 33846467191 scopus 로고    scopus 로고
    • Alternative splicing and differential gene expression in colon cancer detected by a whole genome exon array
    • 10.1186/1471-2164-7-325, 1769375, 17192196
    • Gardina PJ, Clark TA, Shimada B, Staples MK, Yang Q, Veitch J, Schweitzer A, Awad T, Sugnet C, Dee S, et al. Alternative splicing and differential gene expression in colon cancer detected by a whole genome exon array. BMC Genomics 2006, 7:325. 10.1186/1471-2164-7-325, 1769375, 17192196.
    • (2006) BMC Genomics , vol.7 , pp. 325
    • Gardina, P.J.1    Clark, T.A.2    Shimada, B.3    Staples, M.K.4    Yang, Q.5    Veitch, J.6    Schweitzer, A.7    Awad, T.8    Sugnet, C.9    Dee, S.10
  • 12
    • 76949087472 scopus 로고    scopus 로고
    • Normal colon epithelium: a dataset for the analysis of gene expression and alternative splicing events in colon disease
    • 10.1186/1471-2164-11-5, 2823691, 20047688
    • Mojica W, Hawthorn L. Normal colon epithelium: a dataset for the analysis of gene expression and alternative splicing events in colon disease. BMC Genomics 2010, 11:5. 10.1186/1471-2164-11-5, 2823691, 20047688.
    • (2010) BMC Genomics , vol.11 , pp. 5
    • Mojica, W.1    Hawthorn, L.2
  • 13
    • 84873737480 scopus 로고    scopus 로고
    • Next-generation sequencing in the clinic: promises and challenges
    • Xuan J, Yu Y, Qing T, Guo L, Shi L. Next-generation sequencing in the clinic: promises and challenges. Cancer Lett 2012, 340:248-295.
    • (2012) Cancer Lett , vol.340 , pp. 248-295
    • Xuan, J.1    Yu, Y.2    Qing, T.3    Guo, L.4    Shi, L.5
  • 14
    • 57749195712 scopus 로고    scopus 로고
    • RNA-Seq: a revolutionary tool for transcriptomics
    • 10.1038/nrg2484, 2949280, 19015660
    • Wang Z, Gerstein M, Snyder M. RNA-Seq: a revolutionary tool for transcriptomics. Nat Rev Genet 2009, 10:57-63. 10.1038/nrg2484, 2949280, 19015660.
    • (2009) Nat Rev Genet , vol.10 , pp. 57-63
    • Wang, Z.1    Gerstein, M.2    Snyder, M.3
  • 15
    • 84872265962 scopus 로고    scopus 로고
    • Transcriptome analysis using next-generation sequencing
    • 10.1016/j.copbio.2012.09.004, 23020966
    • Mutz K-O, Heilkenbrinker A, Lönne M, Walter J-G, Stahl F. Transcriptome analysis using next-generation sequencing. Curr Opin Biotechnol 2013, 24:22-30. 10.1016/j.copbio.2012.09.004, 23020966.
    • (2013) Curr Opin Biotechnol , vol.24 , pp. 22-30
    • Mutz, K.-O.1    Heilkenbrinker, A.2    Lönne, M.3    Walter, J.-G.4    Stahl, F.5
  • 16
    • 84871593783 scopus 로고    scopus 로고
    • A comprehensive evaluation of alignment algorithms in the context of RNA-Seq
    • 10.1371/journal.pone.0052403, 3530550, 23300661
    • Lindner R, Friedel CC. A comprehensive evaluation of alignment algorithms in the context of RNA-Seq. PLoS ONE 2012, 7:e52403. 10.1371/journal.pone.0052403, 3530550, 23300661.
    • (2012) PLoS ONE , vol.7
    • Lindner, R.1    Friedel, C.C.2
  • 17
    • 84872198346 scopus 로고    scopus 로고
    • Differential analysis of gene regulation at transcript resolution with RNA-seq
    • 10.1038/nbt.2450, 3869392, 23222703
    • Trapnell C, Hendrickson DG, Sauvageau M, Goff L, Rinn JL, Pachter L. Differential analysis of gene regulation at transcript resolution with RNA-seq. Nat Biotechnol 2012, 31:46-53. 10.1038/nbt.2450, 3869392, 23222703.
    • (2012) Nat Biotechnol , vol.31 , pp. 46-53
    • Trapnell, C.1    Hendrickson, D.G.2    Sauvageau, M.3    Goff, L.4    Rinn, J.L.5    Pachter, L.6
  • 22
    • 0032530717 scopus 로고    scopus 로고
    • VEGF and the Fab fragment of a humanized neutralizing antibody: crystal structure of the complex at 2.4 A resolution and mutational analysis of the interface
    • 10.1016/S0969-2126(98)00116-6, 9753694
    • Muller YA, Chen Y, Christinger HW, Li B, Cunningham BC, Lowman HB, de Vos AM. VEGF and the Fab fragment of a humanized neutralizing antibody: crystal structure of the complex at 2.4 A resolution and mutational analysis of the interface. Structure 1998, 6:1153-1167. 10.1016/S0969-2126(98)00116-6, 9753694.
    • (1998) Structure , vol.6 , pp. 1153-1167
    • Muller, Y.A.1    Chen, Y.2    Christinger, H.W.3    Li, B.4    Cunningham, B.C.5    Lowman, H.B.6    de Vos, A.M.7
  • 23
    • 0035062394 scopus 로고    scopus 로고
    • The splice variants of vascular endothelial growth factor (VEGF) and their receptors
    • Robinson CJ, Stringer SE. The splice variants of vascular endothelial growth factor (VEGF) and their receptors. J Cell Sci 2001, 114:853-865.
    • (2001) J Cell Sci , vol.114 , pp. 853-865
    • Robinson, C.J.1    Stringer, S.E.2
  • 25
    • 33947390863 scopus 로고    scopus 로고
    • Alternative splicing in angiogenesis: the vascular endothelial growth factor paradigm
    • 10.1016/j.canlet.2006.08.015, 17027147
    • Ladomery MR, Harper SJ, Bates DO. Alternative splicing in angiogenesis: the vascular endothelial growth factor paradigm. Cancer Lett 2007, 249:133-142. 10.1016/j.canlet.2006.08.015, 17027147.
    • (2007) Cancer Lett , vol.249 , pp. 133-142
    • Ladomery, M.R.1    Harper, S.J.2    Bates, D.O.3
  • 26
    • 77957596291 scopus 로고    scopus 로고
    • The transcription factor E2F1 and the SR protein SC35 control the ratio of pro-angiogenic versus antiangiogenic isoforms of vascular endothelial growth factor-A to inhibit neovascularization in vivo
    • 10.1038/onc.2010.281, 20639906
    • Merdzhanova G, Gout S, Keramidas M, Edmond V, Coll JL, Brambilla C, Brambilla E, Gazzeri S, Eymin B. The transcription factor E2F1 and the SR protein SC35 control the ratio of pro-angiogenic versus antiangiogenic isoforms of vascular endothelial growth factor-A to inhibit neovascularization in vivo. Oncogene 2010, 29:5392-5403. 10.1038/onc.2010.281, 20639906.
    • (2010) Oncogene , vol.29 , pp. 5392-5403
    • Merdzhanova, G.1    Gout, S.2    Keramidas, M.3    Edmond, V.4    Coll, J.L.5    Brambilla, C.6    Brambilla, E.7    Gazzeri, S.8    Eymin, B.9
  • 27
    • 49449116959 scopus 로고    scopus 로고
    • The splicing factor SC35 has an active role in transcriptional elongation
    • 10.1038/nsmb.1461, 2574591, 18641664
    • Lin S, Coutinho-Mansfield G, Wang D, Pandit S, Fu X-D. The splicing factor SC35 has an active role in transcriptional elongation. Nat Struct Mol Biol 2008, 15:819-826. 10.1038/nsmb.1461, 2574591, 18641664.
    • (2008) Nat Struct Mol Biol , vol.15 , pp. 819-826
    • Lin, S.1    Coutinho-Mansfield, G.2    Wang, D.3    Pandit, S.4    Fu, X.-D.5
  • 28
    • 84867364542 scopus 로고    scopus 로고
    • Abnormal expression of the pre-mRNA splicing regulators SRSF1, SRSF2, SRPK1 and SRPK2 in non small cell lung carcinoma
    • 10.1371/journal.pone.0046539, 3468597, 23071587
    • Gout S, Brambilla E, Boudria A, Drissi R, Lantuejoul S, Gazzeri S, Eymin B. Abnormal expression of the pre-mRNA splicing regulators SRSF1, SRSF2, SRPK1 and SRPK2 in non small cell lung carcinoma. PLoS ONE 2012, 7:e46539. 10.1371/journal.pone.0046539, 3468597, 23071587.
    • (2012) PLoS ONE , vol.7
    • Gout, S.1    Brambilla, E.2    Boudria, A.3    Drissi, R.4    Lantuejoul, S.5    Gazzeri, S.6    Eymin, B.7
  • 29
    • 0029295349 scopus 로고
    • Overexpression of the SR proteins ASF/SF2 and SC35 influences alternative splicing in vivo in diverse ways
    • 1369086, 7489505
    • Wang J, Manley JL. Overexpression of the SR proteins ASF/SF2 and SC35 influences alternative splicing in vivo in diverse ways. RNA 1995, 1:335-346. 1369086, 7489505.
    • (1995) RNA , vol.1 , pp. 335-346
    • Wang, J.1    Manley, J.L.2
  • 30
    • 84876153678 scopus 로고    scopus 로고
    • Gene set enrichment analysis of RNA-Seq data: integrating differential expression and splicing
    • Wang X, Cairns MJ. Gene set enrichment analysis of RNA-Seq data: integrating differential expression and splicing. BMC Bioinforma 2013, 14:S16.
    • (2013) BMC Bioinforma , vol.14
    • Wang, X.1    Cairns, M.J.2
  • 31
    • 84879998009 scopus 로고    scopus 로고
    • NURD: an implementation of a new method to estimate isoform expression from non-uniform RNA-seq data
    • Ma X, Zhang X. NURD: an implementation of a new method to estimate isoform expression from non-uniform RNA-seq data. BMC Bioinforma 2013, 14:220.
    • (2013) BMC Bioinforma , vol.14 , pp. 220
    • Ma, X.1    Zhang, X.2
  • 32
    • 77957165656 scopus 로고    scopus 로고
    • Differential detection of alternatively spliced variants of Ciz1 in normal and cancer cells using a custom exon-junction microarray
    • 10.1186/1471-2407-10-482, 2945943, 20831784
    • Rahman FA, Aziz N, Coverley D. Differential detection of alternatively spliced variants of Ciz1 in normal and cancer cells using a custom exon-junction microarray. BMC Cancer 2010, 10:482. 10.1186/1471-2407-10-482, 2945943, 20831784.
    • (2010) BMC Cancer , vol.10 , pp. 482
    • Rahman, F.A.1    Aziz, N.2    Coverley, D.3
  • 33
    • 33645802473 scopus 로고    scopus 로고
    • Therapeutic potential of inhibitory VEGF splice variants
    • 10.2217/14796694.1.4.467, 16556023
    • Bates DO, Harper SJ. Therapeutic potential of inhibitory VEGF splice variants. Future Oncol 2005, 1:467-473. 10.2217/14796694.1.4.467, 16556023.
    • (2005) Future Oncol , vol.1 , pp. 467-473
    • Bates, D.O.1    Harper, S.J.2
  • 34
    • 54549123309 scopus 로고    scopus 로고
    • VEGF-A splicing: the key to anti-angiogenic therapeutics?
    • 10.1038/nrc2505, 2613352, 18923433
    • Harper SJ, Bates DO. VEGF-A splicing: the key to anti-angiogenic therapeutics?. Nat Rev Cancer 2008, 8:880-887. 10.1038/nrc2505, 2613352, 18923433.
    • (2008) Nat Rev Cancer , vol.8 , pp. 880-887
    • Harper, S.J.1    Bates, D.O.2
  • 35
    • 77949318969 scopus 로고    scopus 로고
    • Regulation of vascular endothelial growth factor (VEGF) splicing from pro-angiogenic to anti-angiogenic isoforms: a novel therapeutic strategy for angiogenesis
    • 10.1074/jbc.M109.074930, 2820781, 19906640
    • Nowak DG, Amin EM, Rennel ES, Hoareau-Aveilla C, Gammons M, Damodoran G, Hagiwara M, Harper SJ, Woolard J, Ladomery MR, Bates DO. Regulation of vascular endothelial growth factor (VEGF) splicing from pro-angiogenic to anti-angiogenic isoforms: a novel therapeutic strategy for angiogenesis. J Biol Chem 2010, 285:5532-5540. 10.1074/jbc.M109.074930, 2820781, 19906640.
    • (2010) J Biol Chem , vol.285 , pp. 5532-5540
    • Nowak, D.G.1    Amin, E.M.2    Rennel, E.S.3    Hoareau-Aveilla, C.4    Gammons, M.5    Damodoran, G.6    Hagiwara, M.7    Harper, S.J.8    Woolard, J.9    Ladomery, M.R.10    Bates, D.O.11
  • 36
    • 70450203391 scopus 로고    scopus 로고
    • The anti-angiogenic isoforms of VEGF in health and disease
    • 10.1042/BST0371207, 2882696, 19909248
    • Qiu Y, Hoareau-Aveilla C, Oltean S, Harper SJ, Bates DO. The anti-angiogenic isoforms of VEGF in health and disease. Biochem Soc Trans 2009, 37:1207-1213. 10.1042/BST0371207, 2882696, 19909248.
    • (2009) Biochem Soc Trans , vol.37 , pp. 1207-1213
    • Qiu, Y.1    Hoareau-Aveilla, C.2    Oltean, S.3    Harper, S.J.4    Bates, D.O.5
  • 37
    • 84862682219 scopus 로고    scopus 로고
    • VEGF spliced variants: possible role of anti-angiogenesis therapy
    • 3195439, 22013509
    • Hilmi C, Guyot M, Pages G. VEGF spliced variants: possible role of anti-angiogenesis therapy. J Nucleic Acids 2012, 2012:162692. 3195439, 22013509.
    • (2012) J Nucleic Acids , vol.2012 , pp. 162692
    • Hilmi, C.1    Guyot, M.2    Pages, G.3
  • 39
    • 79955865397 scopus 로고    scopus 로고
    • Identification of an exon 4-deletion variant of epidermal growth factor receptor with increased metastasis-promoting capacity
    • 3084623, 21532887
    • Wang H, Zhou M, Shi B, Zhang Q, Jiang H, Sun Y, Liu J, Zhou K, Yao M, Gu J, et al. Identification of an exon 4-deletion variant of epidermal growth factor receptor with increased metastasis-promoting capacity. Neoplasia 2011, 13:461-471. 3084623, 21532887.
    • (2011) Neoplasia , vol.13 , pp. 461-471
    • Wang, H.1    Zhou, M.2    Shi, B.3    Zhang, Q.4    Jiang, H.5    Sun, Y.6    Liu, J.7    Zhou, K.8    Yao, M.9    Gu, J.10
  • 41
    • 42149152015 scopus 로고    scopus 로고
    • VEGF165b, an antiangiogenic VEGF-A isoform, binds and inhibits bevacizumab treatment in experimental colorectal carcinoma: balance of pro- and antiangiogenic VEGF-A isoforms has implications for therapy
    • 10.1038/sj.bjc.6604308, 2361696,2361696, 18349829
    • Varey AHR, Rennel ES, Qiu Y, Bevan HS, Perrin RM, Raffy S, Dixon AR, Paraskeva C, Zaccheo O, Hassan AB, et al. VEGF165b, an antiangiogenic VEGF-A isoform, binds and inhibits bevacizumab treatment in experimental colorectal carcinoma: balance of pro- and antiangiogenic VEGF-A isoforms has implications for therapy. Br J Cancer 2008, 98:1366-1379. 10.1038/sj.bjc.6604308, 2361696,2361696, 18349829.
    • (2008) Br J Cancer , vol.98 , pp. 1366-1379
    • Varey, A.H.R.1    Rennel, E.S.2    Qiu, Y.3    Bevan, H.S.4    Perrin, R.M.5    Raffy, S.6    Dixon, A.R.7    Paraskeva, C.8    Zaccheo, O.9    Hassan, A.B.10
  • 42
    • 84868316135 scopus 로고    scopus 로고
    • New analysis workflow for MALDI imaging mass spectrometry: application to the discovery and identification of potential markers of childhood absence epilepsy
    • 10.1021/pr3006974, 22994238
    • Lagarrigue M, Alexandrov T, Dieuset G, Perrin A, Lavigne R, Baulac S, Thiele H, Martin B, Pineau C. New analysis workflow for MALDI imaging mass spectrometry: application to the discovery and identification of potential markers of childhood absence epilepsy. J Proteome Res 2012, 11:5453-5463. 10.1021/pr3006974, 22994238.
    • (2012) J Proteome Res , vol.11 , pp. 5453-5463
    • Lagarrigue, M.1    Alexandrov, T.2    Dieuset, G.3    Perrin, A.4    Lavigne, R.5    Baulac, S.6    Thiele, H.7    Martin, B.8    Pineau, C.9
  • 44
    • 30844469526 scopus 로고    scopus 로고
    • E2F1 induces apoptosis and sensitizes human lung adenocarcinoma cells to death-receptor-mediated apoptosis through specific downregulation of c-FLIPshort
    • 10.1038/sj.cdd.4401739, 16052233
    • Salon C, Eymin B, Micheau O, Chaperot L, Plumas J, Brambilla C, Brambilla E, Gazzeri S. E2F1 induces apoptosis and sensitizes human lung adenocarcinoma cells to death-receptor-mediated apoptosis through specific downregulation of c-FLIPshort. Cell Death Differ 2006, 13:260-272. 10.1038/sj.cdd.4401739, 16052233.
    • (2006) Cell Death Differ , vol.13 , pp. 260-272
    • Salon, C.1    Eymin, B.2    Micheau, O.3    Chaperot, L.4    Plumas, J.5    Brambilla, C.6    Brambilla, E.7    Gazzeri, S.8
  • 45
    • 34548027321 scopus 로고    scopus 로고
    • E2F1, Skp2 and cyclin E oncoproteins are upregulated and directly correlated in high-grade neuroendocrine lung tumors
    • 10.1038/sj.onc.1210499, 17471231
    • Salon C, Merdzhanova G, Brambilla C, Brambilla E, Gazzeri S, Eymin B. E2F1, Skp2 and cyclin E oncoproteins are upregulated and directly correlated in high-grade neuroendocrine lung tumors. Oncogene 2007, 26:6927-6936. 10.1038/sj.onc.1210499, 17471231.
    • (2007) Oncogene , vol.26 , pp. 6927-6936
    • Salon, C.1    Merdzhanova, G.2    Brambilla, C.3    Brambilla, E.4    Gazzeri, S.5    Eymin, B.6
  • 46
    • 84876297094 scopus 로고    scopus 로고
    • Gene-and exon-expression profiling reveals an extensive LPS-induced response in immune cells in patients with cirrhosis
    • 10.1016/j.jhep.2012.12.025, 23321315
    • Gandoura S, Weiss E, Rautou P-E, Fasseu M, Gustot T, Lemoine F, Hurtado-Nedelec M, Hego C, Vadrot N, Elkrief L. Gene-and exon-expression profiling reveals an extensive LPS-induced response in immune cells in patients with cirrhosis. J Hepatol 2013, 58:936-948. 10.1016/j.jhep.2012.12.025, 23321315.
    • (2013) J Hepatol , vol.58 , pp. 936-948
    • Gandoura, S.1    Weiss, E.2    Rautou, P.-E.3    Fasseu, M.4    Gustot, T.5    Lemoine, F.6    Hurtado-Nedelec, M.7    Hego, C.8    Vadrot, N.9    Elkrief, L.10
  • 47
    • 84871343422 scopus 로고    scopus 로고
    • Global profiling of alternative splicing events and gene expression regulated by hnRNPH/F
    • 10.1371/journal.pone.0051266, 3524136, 23284676
    • Wang E, Aslanzadeh V, Papa F, Zhu H, de la Grange P, Cambi F. Global profiling of alternative splicing events and gene expression regulated by hnRNPH/F. PLoS ONE 2012, 7:e51266. 10.1371/journal.pone.0051266, 3524136, 23284676.
    • (2012) PLoS ONE , vol.7
    • Wang, E.1    Aslanzadeh, V.2    Papa, F.3    Zhu, H.4    de la Grange, P.5    Cambi, F.6
  • 48
    • 0035710746 scopus 로고    scopus 로고
    • Analysis of relative gene expression data using real-time quantitative PCR and the 2 - ΔΔCT method
    • 10.1006/meth.2001.1262, 11846609
    • Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2 - ΔΔCT method. Methods 2001, 25:402-408. 10.1006/meth.2001.1262, 11846609.
    • (2001) Methods , vol.25 , pp. 402-408
    • Livak, K.J.1    Schmittgen, T.D.2


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