Establishment of a rapid, inexpensive protocol for extraction of high quality RNA from small amounts of strawberry plant tissues and other recalcitrant fruit crops

Gene

Volumn 537, Issue 1, 2014, Pages 169-173

  Christou, Anastasis ^a,b   Georgiadou, Egli C ^a   Filippou, Panagiota ^a   Manganaris, George A ^a   Fotopoulos, Vasileios ^a  

^a CYPRUS UNIVERSITY OF TECHNOLOGY   (Cyprus)

^b AGRICULTURAL RESEARCH INSTITUTE   (Cyprus)

Author keywords

Fragaria ananassa; Genomic DNA extraction; Polyphenols; Polysaccharides; Protocol; Total RNA extraction

Indexed keywords

GENOMIC DNA; NUCLEIC ACID; RIBONUCLEASE A; RNA;

ARTICLE; CHEMICAL MODIFICATION; CROP; GENE AMPLIFICATION; HOUSEKEEPING GENE; KIWIFRUIT; NONHUMAN; OLIVE; PLANT LEAF; PLANT ROOT; PLANT TISSUE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RIBONUCLEIC ACID MODIFICATION; RNA EXTRACTION; STRAWBERRY;

ACTINIDIA DELICIOSA; FRAGARIA X ANANASSA;

EB; EXTRACTION BUFFER; FRAGARIA×ANANASSA; GENOMIC DNA EXTRACTION; NAOAC; PCI; PHENOL: CHLOROFORM: ISOAMYL ALCOHOL; POLYPHENOLS; POLYSACCHARIDES; PROTOCOL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RT-PCR; SDS; SODIUM ACETATE; SODIUM DODECYL SULFATE; TOTAL RNA EXTRACTION;

ACTINIDIA; CHEMICAL FRACTIONATION; FRAGARIA; FRUIT; OLEA; PLANT LEAVES; PLANT ROOTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, PLANT;

EID: 84892858354     PISSN: 03781119     EISSN: 18790038     Source Type: Journal    
DOI: 10.1016/j.gene.2013.11.066     Document Type: Article

References (30)

1. Asami D.K., Hong Y.J., Barrett D.M., Mitchell A.E. Comparison of the total phenolic and ascorbic acid content of freeze-dried and air-dried marionberry, strawberry, and corn grown using conventional, organic, and sustainable agricultural practices. J. Agric. Food Chem. 2003, 51:1237-1241.
2. Asif M.H., Dhawan P., Nath P. A simple procedure for the isolation of high quality RNA from ripening banana fruit. Plant Mol. Biol. Rep. 2000, 18:109-115.
3. Berendzen K., et al. A rapid and versatile combined DNA/RNA extraction protocol and its application to the analysis of a novel DNA marker set polymorphic between Arabidopsis thaliana ecotypes Col-0 and Landsberg erecta. Plant Methods 2005, 1:4.
4. Chai Y., et al. Brassinosteroid is involved in strawberry fruit ripening. Plant Growth Regul. 2013, 69:63-69.
5. Chang S., Puryear J., Cairney J. A simple and efficient method for isolating RNA from pine trees. Plant Mol. Biol. Rep. 1993, 11:113-116.
6. Chen J., et al. Molecular characterization of a strawberry FaASR gene in relation to fruit ripening. PLoS ONE 2011, 6:e24649.
7. Christou A., Manganaris G.A., Papadopoulos I., Fotopoulos V. Hydrogen sulphide induces systemic tolerance to salinity and non-ionic osmotic stress in strawberry plants through modification of reactive species biosynthesis and transcriptional regulation of multiple defence pathways. J. Exp. Bot. 2013, 64:1953-1966.
8. Davis A.R., Levi A., Kim S., King S.R., Hernandez A. RNA extraction method from fruit tissue high in water and sugar. HortSci. 2006, 41:1292-1294.
9. Demeke T., Adams R.P. The effects of plant polysaccharides and buffer additives on PCR. Biotechniques 1992, 12:332-334.
10. Henderson D.C., Hammond J. CKC: isolation of nucleic acids from a diversity of plants using CTAB and silica columns. Mol. Biotechnol. 2013, 53:109-117.
11. Hernández L.M., Padilla M.N., Mancha M., Martínez-Rivas J.M. Expression analysis identifies FAD2-2 as the olive oléate desaturase gene mainly responsible for the linoleic acid content in virgin olive oil. J. Agric. Food Chem. 2009, 57:6199-6206.
12. Hu C.G., Honda C., Kita M., Zhang Z., Tsuda T., Moriguchi T. A simple protocol for RNA isolation from fruit trees containing high levels of polysaccharides and polyphenol compounds. Plant Mol. Biol. Rep. 2002, 20:69a-69g.
13. Japelaghi R., Haddad R., Garoosi G.A. Rapid and efficient isolation of high quality nucleic acids from plant tissues rich in polyphenols and polysaccharides. Mol. Biotechnol. 2011, 49:129-137.
14. Lin X., Xiao M., Luo Y., Wang J., Wang H. The effect of RNAi-induced silencing of FaDFR on anthocyanin metabolism in strawberry (Fragaria x ananassa) fruit. Sci. Hortic. 2013, 160:123-128.
15. Martínez G.A., Civello P.M. Effect of heat treatments on gene expression and enzyme activities associated to cell wall degradation in strawberry fruit. Postharvest Biol. Technol. 2008, 49:38-45.
16. Mazzara M., James D. The influence of photoperiodic growth condition on isolation of RNA from strawberry (Fragaria×ananassa duch.) tissue. Mol. Biotechnol. 2000, 15:237-241.
17. Miyawaki K., et al. Establishment of a novel system to elucidate the mechanisms underlying light-induced ripening of strawberry fruit with an Agrobacterium-mediated RNAi technique. Plant Biotechnol. 2012, 29:271-277.
18. Moreira D. Efficient removal of PCR inhibitors using agarose-embedded DNA preparations. Nucleic Acids Res. 1998, 26:3309-3310.
19. Mortaji Z., Tavakkol Afshari R., Alizadeh H., Yazdi Samadi B. RNA isolation and expression from different dormant and after-ripened wheat (Triticum aestivum) seed tissues rich in polysaccharides and proteins. Asian J. Plant Sci. 2008, 7:201-206.
20. Moser C., Gatto P., Moser M., Pindo M., Velasco R. Isolation of functional RNA from small amounts of different grape and apple tissues. Mol. Biotechnol. 2004, 26:95-99.
21. Palomer X., et al. Antisense down-regulation of strawberry endo-β-(1,4)-glucanase genes does not prevent fruit softening during ripening. Plant Sci. 2006, 171:640-646.
22. Pombo M.A., Martínez G.A., Civello P.M. Cloning of FaPAL6 gene from strawberry fruit and characterization of its expression and enzymatic activity in two cultivars with different anthocyanin accumulation. Plant Sci. 2011, 181:111-118.
23. Samanta P., Sadhukhan S., Das S., Joshi A., Sen S., Basu A. Isolation of RNA from field-grown jute (Corchorus capsularis) plant in different developmental stages for effective downstream molecular analysis. Mol. Biotechnol. 2011, 49:109-115.
24. Sambrook J., Russell D.W. Molecular Cloning: A Laboratory Manual 2001, Cold Spring Harbour Laboratory Press, New York.
25. Sharma A.D., Gill P.K., Singh P. RNA isolation from plant tissues rich in polysaccharides. Anal. Biochem. 2003, 314:319-321.
26. Smart M., Roden L.C. A small-scale RNA isolation protocol useful for high-throughput extractions from recalcitrant plants. S. Afr. J. Bot. 2010, 76:375-379.
27. Tulipani S., et al. Antioxidants, phenolic compounds, and nutritional quality of different strawberry genotypes. J. Agric. Food Chem. 2008, 56:696-704.
28. Wan C.Y., Wilkins T.A. A modified hot borate method significantly enhances the yield of high-quality RNA from cotton (Gossypium hirsutum L.). Anal. Biochem. 1994, 223:7-12.
29. Wang L., Stegemann J.P. Extraction of high quality RNA from polysaccharide matrices using cetlytrimethylammonium bromide. Biomaterials 2010, 31:1612-1618.
30. Yin X., Allan A.C., Chen K., Ferguson I.B. Kiwifruit EIL and ERF genes involved in regulating fruit ripening. Plant Physiol. 2010, 153:1280-1292.