Bottlenecks in erucic acid accumulation in genetically engineered ultrahigh erucic acid Crambe abyssinica

Plant Biotechnology Journal

Volumn 12, Issue 2, 2014, Pages 193-203

  Guan, Rui ^a   Lager, Ida ^a   Li, Xueyuan ^a   Stymne, Sten ^a   Zhu, Li Hua ^a  

^a SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

Author keywords

Acylation; Biosynthesis; Crambe abyssinica; Enzymes; Erucic acid; Seed oil

Indexed keywords

BRASSICA JUNCEA; BRASSICA NAPUS; CARTHAMUS TINCTORIUS; CRAMBE ABYSSINICA;

CARBON; CHLOROPHYLL; ENZYME; ERUCIC ACID; FATTY ACID; GLYCEROL; VEGETABLE OIL;

ACYLATION; ARTICLE; BIOSYNTHESIS; BRASSICA; BRASSICA RAPA; CRAMBE; CRAMBE ABYSSINICA; GENETIC ENGINEERING; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; PLANT SEED; TIME; TRANSGENIC PLANT;

ACYLATION; BIOSYNTHESIS; CRAMBE ABYSSINICA; ENZYMES; ERUCIC ACID; SEED OIL;

ACYLATION; BRASSICA; BRASSICA RAPA; CARBON RADIOISOTOPES; CHLOROPHYLL; CRAMBE PLANT; ERUCIC ACIDS; FATTY ACIDS; GENETIC ENGINEERING; GLYCEROL; PLANT OILS; PLANTS, GENETICALLY MODIFIED; SEEDS; TIME FACTORS;

EID: 84892841454     PISSN: 14677644     EISSN: 14677652     Source Type: Journal    
DOI: 10.1111/pbi.12128     Document Type: Article

References (42)

1. Arnon, D.I. (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol. 24, 1-15.
2. Bafor, M., Jonsson, L., Stobart, A. and Stymne, S. (1990) Regulation of triacylglycerol biosynthesis in embryos and microsomal preparations from the developing seeds of Cuphea lanceolata. Biochem. J. 272, 31-38.
3. Bao, X., Pollard, M. and Ohlrogge, J. (1998) The biosynthesis of erucic acid in developing embryos of Brassica rapa. Plant Physiol. 118, 183-190.
4. Bates, P.D. and Browse, J. (2011) The pathway of triacylglycerol synthesis through phosphatidylcholine in Arabidopsis produces a bottleneck for the accumulation of unusual fatty acids in transgenic seeds. Plant J. 68, 387-399.
5. Bates, P.D. and Browse, J. (2012) The significance of different diacylgycerol synthesis pathways on plant oil composition and bioengineering. Front. Plant Sci. 3, 147.
6. Bates, P.D., Ohlrogge, J.B. and Pollard, M. (2007) Incorporation of newly synthesized fatty acids into cytosolic glycerolipids in pea leaves occurs via acyl editing. J. Biol. Chem. 282, 31206-31216.
7. Bates, P.D., Durrett, T.P., Ohlrogge, J.B. and Pollard, M. (2009) Analysis of acyl fluxes through multiple pathways of triacylglycerol synthesis in developing soybean embryos. Plant Physiol. 150, 55-72.
8. Bates, P.D., Fatihi, A., Snapp, A.R., Carlsson, A.S. and Lu, C. (2012) Acyl editing and headgroup exchange are the major mechanisms that direct polyunsaturated fatty acid flux into triacylglycerols. Plant Physiol. 160, 1530-1539.
9. Bates, P.D., Stymne, S. and Ohlrogge, J. (2013) Biochemical pathways in seed oil synthesis. Curr. Opin. Plant Biol. 16, 358-364.
10. Bhardwaj, H.L. and Hamama, A.A. (2003) Accumulation of glucosinolate, oil, and erucic acid in developing Brassica seeds. Ind. Crop Prod. 17, 47-51.
11. Bligh, E.G. and Dyer, W.J. (1959) A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37, 911-917.
12. Carlsson, A.S., Yilmaz, J.L., Green, A.G., Stymne, S. and Hofvander, P. (2011) Replacing fossil oil with fresh oil-with what and for what? Eur. J. Lipid Sci. Technol. 113, 812-831.
13. Dahlqvist, A., Ståhl, U., Lenman, M., Banas, A., Lee, M., Sandager, L., Ronne, H. and Stymne, S. (2000) Phospholipid: diacylglycerol acyltransferase: an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants. Proc. Natl Acad. Sci. USA, 97, 6487-6492.
14. Dyer, J.M., Stymne, S., Green, A.G. and Carlsson, A.S. (2008) High-value oils from plants. Plant J. 54, 640-655.
15. Frentzen, M. (1993) Acyltransferases and triacylglycerols. In Lipid Metabolism in Plants(Moore, T.S. Jr, ed.), pp. 195-220. Boca Raton, FL: CRC Press.
16. Griffiths, G., Stobart, A.K. and Stymne, S. (1985) The acylation of sn-glycerol 3-phosphate and the metabolism of phosphatidate in microsomal preparations from the developing cotyledons of safflower (Carthamus tinctorius L.) seed. Biochem. J. 230, 379-388.
17. Hanke, C., Wolter, F.P., Coleman, J., Peterek, G. and Frentzen, M. (1995) A plant acyltransferase involved in triacylglycerol biosynthesis complements an Escherichia Coli sn-1-acylglycerol-3-phosphate acyltransferase mutant. Eur. J. Biochem. 232, 806-810.
18. Hlousek-Radojcic, A., Imai, H. and Jaworski, J.G. (1995) Oleoyl-CoA is not an immediate substrate for fatty acid elongation in developing seeds of Brassica napus. Plant J. 8, 803-809.
19. Hu, Z., Ren, Z. and Lu, C. (2012) The phosphatidylcholine diacylglycerol cholinephosphotransferase is required for efficient hydroxy fatty acid accumulation in transgenic Arabidopsis. Plant Physiol. 158, 1944-1954.
20. Ichihara, K., Murota, N. and Fujii, S. (1990) Intracellular translocation of phosphatidate phosphatase in maturing safflower seeds: a possible mechanism of feedforward control of triacylglycerol synthesis by fatty acids. Biochim. Biophys. Acta, 1043, 227-234.
21. Kanda, P. and Wells, M.A. (1981) Facile acylation of glycerophosphocholine catalyzed by trifluoroacetic anhydride. J. Lipid Res. 22, 877-879.
22. Kelly, A.A., Shaw, E., Powers, S.J., Kurup, S. and Eastmond, P.J. (2013) Suppression of the SUGAR-DEPENDENT1 triacylglcycerol lipase family during seed development enhances oil uield in oil seed rape (Brassica napus L.). Plant Biotechnol. J. 11, 355-361.
23. Kennedy, E.P. (1961) Biosynthesis of complex lipids. Fed. Proc. 20, 934-940.
24. Knutzon, D.S., Hayes, T.R., Wyrick, A., Xiong, H., Davies, H.M. and Voelker, T.A. (1999) Lysophosphatidic acid acyltransferase from coconut endosperm mediates the insertion of laurate at the sn-2 position of triacylglycerols in lauric rapeseed oil and can increase total laurate levels. Plant Physiol. 120, 739-746.
25. Li, X., van Loo, E., Gruber, J., Fan, J., Guan, R., Frentzen, M., Stymne, S. and Zhu, L. (2012) Development of ultra-high erucic acid oil in the industrial oil crop Crambe abyssinica. Plant Biotechnol. J. 10, 862-870.
26. Li-Beisson, Y., Shorrosh, B., Beisson, F., Andersson, M.X., Arondel, V., Bates, P.D., Baud, S., Bird, D., Debono, A., Durrett, T.P., Franke, R.B., Graham, I.A., Katayama, K., Kelly, A.A., Larson, T., Markham, J.E., Miquel, M., Molina, I., Nishida, I., Rowland, O., Samuels, L., Schmid, K.M., Wada, H., Welti, R., Xu, C., Zallot, R. and Ohlrogge, J. (2013) Acyl-lipid metabolism. Arabidopsis Book 11, e0161.
27. Löhden, I. and Frentzen, M. (1992) Triacylglycerol biosynthesis in developing seeds of Tropaeolum majus L. and Limnanthes douglasii R. Br. Planta, 188, 215-224.
28. Lu, C., Xin, Z., Ren, Z. and Miquel, M. (2009) An enzyme regulating triacylglycerol composition is encoded by the ROD1 gene of Arabidopsis. Proc. Natl Acad. Sci. USA, 106, 18837-18842.
29. Millar, A.A., Wrischer, M. and Kunst, L. (1998) Accumulation of very-long-chain fatty acids in membrane glycerolipids is associated with dramatic alterations in plant morphology. Plant Cell, 10, 1889-1902.
30. Sánchez, M., Nicholls, D.G. and Brindley, D.N. (1973) The relationship between palmitoylcoenzymeA synthetase activity and esterification of sn-glycerol 3-phosphate in rat liver mitochondria. Biochem. J. 132, 697-706.
31. Slack, C.R., Campbell, L.C., Browse, J.A. and Roughan, P.G. (1983) Some evidence for the reversibility of the cholinephosphotransferase-catalysed reaction in developing linseed cotyledons in vivo. Biochim. Biophys. Acta, 754, 10-20.
32. Slack, C.R., Roughan, P.G., Browse, J.A. and Gardiner, S.E. (1985) Some properties of cholinephosphotransferase from developing safflower cotyledons. Biochim. Biophys. Acta, 833, 438-448.
33. Ståhl, U., Banas, A. and Stymne, S. (1995) Plant microsomal phospholipid acyl hydrolases have selectivities for uncommon fatty acids. Plant Physiol. 107, 953-962.
34. Ståhl, U., Carlsson, A.S., Lenman, M., Dahlqvist, A., Huang, B., Banaś, W., Banaś, A. and Stymne, S. (2004) Cloning and functional characterization of a phospholipid: diacylglycerol acyltransferase from Arabidopsis. Plant Physiol. 135, 1324-1335.
35. Stobart, A.K. and Stymne, S. (1985) The regulation of the fatty-acid composition of the triacylglycerols in microsomal preparations from avocado mesocarp and the developing cotyledons of safflower. Planta, 163, 119-125.
36. Stobart, A.K., Mancha, M., Lenman, M., Dahlqvist, A. and Stymne, S. (1997) Triaclglycerols are synthesised and utilized by transacylation reactions in microsomal preparations of developing safflower (Carthamus tinctorius L.) seeds. Planta, 203, 58-66.
37. Stymne, S. and Stobart, A.K. (1984) Evidence for the reversibility of the acyl-CoA: lysophosphatidylcholine acyltransferase in microsomal preparations from developing safflower (Carthamus tinctorius L.) cotyledons and rat liver. Biochem. J. 223, 305-314.
38. Stymne, S. and Stobart, A.K. (1987) Triacylglycerol biosynthesis. In The Biochemistry of Plants, Vol. 9 (Stumpf, P.K. and Conn, E.E., eds), pp. 175-214. New York: Academic Press.
39. Stymne, S., Stobart, A.K. and Glad, G. (1983) The role of the acyl-CoA pool in the synthesis of polyunsaturated 18-carbon fatty acids and triacylglycerol production in the microsomes of developing safflower seeds. Biochim. Biophys. Acta, 752, 198-208.
40. Temple-Heald, C. (2004) High erucic oil: its production and uses. In Rapeseed and Canola Oil: Productions, Properties and Uses (Gunstone, F.D., ed.), pp. 111-129. Oxford: Blackwell Publishing, CRC Press.
41. Van Erp, H., Bates, P.D., Burgal, J. and Shockey, J. (2011) Castor phospholipid: diacylglycerol acyltransferase facilitates efficient metabolism of hydroxy fatty acids in transgenic Arabidopsis. Plant Physiol. 155, 683-693.
42. Zhang, M., Fan, J., Taylor, D.C. and Ohlrogge, J.B. (2009) DGAT1 and PDAT1 acyltransferases have overlapping functions in Arabidopsis triacylglycerol biosynthesis and are essential for normal pollen and seed development. Plant Cell, 21, 3885-3901.