Disruption of LptA oligomerization and affinity of the LptA-LptC interaction

Protein Science

Volumn 22, Issue 11, 2013, Pages 1639-1645

  Schultz, Kathryn M ^a   Feix, Jimmy B ^a   Klug, Candice S ^a  

^a MEDICAL COLLEGE OF WISCONSIN   (United States)

Author keywords

Dissociation constants; EPR spectroscopy; ITC; LPS binding protein; LptA; LptC; Protein oligomerization

Indexed keywords

GLUTAMINE; LIPOPOLYSACCHARIDE; LIPOPOLYSACCHARIDE BINDING PROTEIN; LPTA PROTEIN; LPTC PROTEIN; LYSINE; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CIRCULAR DICHROISM; ELECTRON SPIN RESONANCE; HYDROGEN BOND; ISOTHERMAL TITRATION CALORIMETRY; OLIGOMERIZATION; POINT MUTATION; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN SECONDARY STRUCTURE;

DISSOCIATION CONSTANTS; EPR SPECTROSCOPY; ITC; LPS BINDING PROTEIN; LPTA; LPTC; PROTEIN OLIGOMERIZATION;

AMINO ACID SEQUENCE; BINDING SITES; CALORIMETRY; CARRIER PROTEINS; CIRCULAR DICHROISM; ELECTRON SPIN RESONANCE SPECTROSCOPY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; POINT MUTATION; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY;

EID: 84892738005     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.2369     Document Type: Article

References (20)

1. Sperandeo P, Deho G, Polissi A (2009) The lipopolysaccharide transport system of Gram-negative bacteria. Biochim Biophys Acta 1791:594-602.
2. Bos MP, Tefsen B, Geurtsen J, Tommassen J (2004) Identification of an outer membrane protein required for the transport of lipopolysaccharide to the bacterial cell surface. Proc Natl Acad Sci U S A 101:9417-9422.
3. Wu T, McCandlish AC, Gronenberg LS, Chng SS, Silhavy TJ, Kahne D (2006) Identification of a protein complex that assembles lipopolysaccharide in the outer membrane of Escherichia coli. Proc Natl Acad Sci USA 103:11754-11759.
4. Sperandeo P, Cescutti R, Villa R, Di Benedetto C, Candia D, Deho G, Polissi A (2007) Characterization of lptA and lptB, two essential genes implicated in lipopolysaccharide transport to the outer membrane of Escherichia coli. J Bacteriol 189:244-253.
5. Sperandeo P, Lau FK, Carpentieri A, De CC, Molinaro A, Deho G, Silhavy TJ, Polissi A (2008) Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coli. J Bacteriol 190:4460-4469.
6. Ruiz N, Gronenberg LS, Kahne D, Silhavy TJ (2008) Identification of two inner-membrane proteins required for the transport of lipopolysaccharide to the outer membrane of Escherichia coli. Proc Natl Acad Sci U S A 105:5537-5542.
7. Narita S, Tokuda H (2009) Biochemical characterization of an ABC transporter LptBFGC complex required for the outer membrane sorting of lipopolysaccharides. FEBS Lett 583:2160-2164.
8. Ruiz N, Kahne D, Silhavy TJ (2009) Transport of lipopolysaccharide across the cell envelope: the long road of discovery. Nat Rev Microbiol 7:677-683.
9. Chng SS, Ruiz N, Chimalakonda G, Silhavy TJ, Kahne D (2010) Characterization of the two-protein complex in Escherichia coli responsible for lipopolysaccharide assembly at the outer membrane. Proc Natl Acad Sci U S A 107:5363-5368.
10. Freinkman E, Chng SS, Kahne D (2011) The complex that inserts lipopolysaccharide into the bacterial outer membrane forms a two-protein plug-and-barrel. Proc Natl Acad Sci U S A 108:2486-2491.
11. Chng SS, Gronenberg LS, Kahne D (2010) Proteins required for lipopolysaccharide assembly in Escherichia coli form a transenvelope complex. Biochemistry 49: 4565-4567.
12. Suits MD, Sperandeo P, Deho G, Polissi A, Jia Z (2008) Novel structure of the conserved gram-negative lipopolysaccharide transport protein A and mutagenesis analysis. J Mol Biol 380:476-488.
13. Tran AX, Dong C, Whitfield C (2010) Structure and functional analysis of LptC, a conserved membrane protein involved in the lipopolysaccharide export pathway in Escherichia coli. J Biol Chem 285:33529-33539.
14. Merten JA, Schultz KM, Klug CS (2012) Concentration-dependent oligomerization and oligomeric arrangement of LptA. Protein Sci 21:211-218.
15. Sperandeo P, Villa R, Martorana AM, Samalikova M, Grandori R, Deho G, Polissi A (2011) New insights into the Lpt machinery for lipopolysaccharide transport to the cell surface: LptA-LptC interaction and LptA stability as sensors of a properly assembled transenvelope complex. J Bacteriol 193:1042-1053.
16. Freinkman E, Okuda S, Ruiz N, Kahne D (2012) Regulated assembly of the transenvelope protein complex required for lipopolysaccharide export. Biochemistry 51:4800-4806.
17. Bowyer A, Baardsnes J, Ajamian E, Zhang L, Cygler M (2011) Characterization of interactions between LPS transport proteins of the Lpt system. Biochem Biophys Res Commun 404:1093-1098.
18. Tran AX, Trent MS, Whitfield C (2008) The LptA protein of Escherichia coli is a periplasmic lipid A-binding protein involved in the lipopolysaccharide export pathway. J Biol Chem 283:20342-20349.
19. Pannier M, Veit S, Godt A, Jeschke G, Spiess HW (2000) Dead-time free measurement of dipole-dipole interactions between electron spins. J Magn Reson 142:331-340.
20. Jeschke G, Chechik V, Ionita P, Godt A, Zimmermann H, Banham J, Timmel C, Hilger D, Jung H (2006) DeerAnalysis2006: a comprehensive software package for analyzing pulsed ELDOR data. Appl Magn Reson 30:473-498.