ITRAQ-labeling for biomarker discovery

Methods in Molecular Biology

Volumn 1002, Issue , 2013, Pages 105-114

  DeSouza, Leroi V ^a   Voisin, Sébastien N ^a   Siu, K W Michael ^a  

^a YORK UNIVERSITY   (Canada)

Author keywords

Biomarker discovery; ITRAQ labeling; LC MS MS analysis; Mass tagging; Relative quanti fi cation; Reversed phase; Strong cation exchange; Trypsin digestion

Indexed keywords

REAGENT; TRYPSIN; TUMOR MARKER;

FRACTIONATION; HUMAN; ITRAQ; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN DATABASE; REVERSED PHASE LIQUID CHROMATOGRAPHY; REVIEW;

HUMANS; ISOTOPE LABELING; MASS SPECTROMETRY; NEOPLASMS; PROTEINS; PROTEOME; PROTEOMICS; TUMOR MARKERS, BIOLOGICAL;

EID: 84892727013     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-360-2_9     Document Type: Review

References (6)

1. Ong S-E, Blagoev B, Kratchmarova I et al (2002) Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics. Mol Cell Proteomics 1:376-386
2. Yao X, Freas A, Ramirez J, Demirev PA, Fenselau C (2001) Proteolytic 18O labeling for comparative proteomics: model studies with two serotypes of adenovirus. Anal Chem 73:2836-2842
3. Gygi SP, Rist B, Gerber SA, Turecek F, Gelb MH, Aebersold R (1999) Quantitative analysis of complex protein mixtures using isotope coded af fi nity tags. Nat Biotechnol 17:994-999
4. Tandem mass tag (TMT) (2008) http://www.piercenet.com/products/browse.cfm fl dID= C5A5F2FF-5056-8A76-4E4C-971BF496D176
5. Ross PL, Huang YN, Marchese JN et al (2004) Multiplexed protein quantitation in saccharomyces cerevisiae using amine-reactive isobaric tagging reagents. Mol Cell Proteomics 3: 1154-1169
6. Elliot MH, Smith DS, Parker CE, Borchers C (2009) Current trends in quantitative proteomics. J Mass Spectrom 44:1637-1660