Droplet based directed evolution of yeast cell factories doubles production of industrial enzymes

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

Volumn 2, Issue , 2013, Pages 1270-1272

  Sjostrom, Staffan L ^a   Bai, Yunpeng ^a   Huang, Mingtao ^b   Nielsen, Jens ^a,b,c   Joensson, Haakan N ^a   Svahn, Helene Andersson ^a  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^b CHALMERS UNIVERSITY OF TECHNOLOGY   (Sweden)

^c TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

Cell factories; Directed evolution; Droplet microfluidics; Enzymes; High throughput

Indexed keywords

AMYLASES; ENZYMES; THROUGHPUT; YEAST;

AMYLASE PRODUCTION; DIRECTED EVOLUTION; DROPLET MICROFLUIDICS; FALSE POSITIVE RATES; HIGH THROUGHPUT; INDUSTRIAL ENZYMES; MICRO-DROPLET; YEAST STRAIN;

DROPS;

EID: 84892513319     PISSN: None     EISSN: None     Source Type: Conference Proceeding    
DOI: None     Document Type: Conference Paper

References (4)

1. B. Kintses, et al., "Picoliter cell lysate assays in microfluidic droplet compartments for directed enzyme evolution", Chemistry & Biology, vol. 19, 1001-1009, Aug. 2012.
2. J. J. Agresti, et al., "Ultrahigh-throughput screening in drop-based microfluidics for directed evolution", Proceedings of the National Academy of Sciences of the United States of America, vol. 107, 4004-4009, Dec. 2009.
3. E. Nevogit, "Progress in metabolic engineering of Saccharomyces cerevisiae", Microbiol Mol Biol Rev., vol. 186, pp. 379-412, Sept. 2008.
4. J. C. Baret, et al, "Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity", Lab On a Chip, vol 9, 1850-1858, Apr. 2009.