A novel insecticidal GroEL protein from Xenorhabdus nematophila confers insect resistance in tobacco

Transgenic Research

Volumn 23, Issue 1, 2014, Pages 99-107

  Kumari, Punam ^a   Kant, Shashi ^a   Zaman, Shazmira ^a   Mahapatro, Gagan Kumar ^b   Banerjee, Nirupama ^a   Sarin, Neera Bhalla ^a  

^a JAWAHARLAL NEHRU UNIVERSITY   (India)

^b INDIAN AGRICULTURAL RESEARCH INSTITUTE   (India)

Author keywords

Chitin binding activity; Helicoverpa armigera; Insect resistance; Transgenic tobacco; Xenorhabdus nematophila; XnGroEL

Indexed keywords

AGROBACTERIUM; BACTERIA (MICROORGANISMS); HELICOVERPA ARMIGERA; HEXAPODA; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM; XENORHABDUS NEMATOPHILA;

CHAPERONIN 60; ENDOTOXIN;

ANIMAL; ARTICLE; BIOLOGICAL PEST CONTROL; GENETICS; INSECT; METABOLISM; PARASITOLOGY; PATHOGENICITY; TOBACCO; TRANSGENIC PLANT; XENORHABDUS;

ANIMALS; CHAPERONIN 60; ENDOTOXINS; INSECTS; PEST CONTROL, BIOLOGICAL; PLANTS, GENETICALLY MODIFIED; TOBACCO; XENORHABDUS;

EID: 84892444264     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11248-013-9734-3     Document Type: Article

References (46)

1. Akhurst RJ, Dunphy GB (1988) Symbiotically associated entomopathogenic bacteria, nematodes and their insect hosts. In: Beckage N, Thompson S, Federici B (eds) Parasites and pathogens of insects, vol 2. Academic, New York, pp 1-23.
2. Akhurst RJ, Dunphy GB, Beckage N, Thompson S, Federici B (1993) Parasites and pathogens of insects, vol 2. Academic, New York, pp 1-23.
3. Bedding RA, Akhurst RJ (1975) Nematodes and their biological control of insect pests. Nematologia 21: 109-110.
4. Boemare NE, Akhrust RJ (1988) Biochemical and physiological characterization of colony variants in Xenorhabdus ssp. (Enteriobacteriaciae). J Gen Microbiol 134: 751-761.
5. Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
6. Brookes G, Barfoot P (2009) Global impact of biotech crops: socio-economic and environmental effects, 1996-2007. Outlooks Pest Manag 20: 258-264.
7. Christou P, Twyman RM (2004) The potential of genetically enhanced plants to address food insecurity. Nutr Res Rev 17: 23-42.
8. Christou P, Capell T, Kohli A, Gatehouse J, Gatehouse A (2006) Recent developments and future prospects in insect pest control in transgenic crops. Trends Plant Sci 11: 302-308.
9. Dukan S, Nystrom T (1999) Oxidative stress defense and deterioration of growth arrested Escherichia coli cells. J Biol Chem 274: 26027-26032.
10. Fayet O, Ziegelhoffer T, Georgopoulos C (1989) The groES and groEL heat shock gene products of Escherichia coli are essential for bacterial growth at all temperatures. J Bacteriol 171: 1379-1385.
11. Fink AL (1999) Chaperone mediated protein folding. Physiol Rev 79: 425-449.
12. Forst S, Nealson K (1996) Molecular biology of the symbiotic-pathogenic bacteria Xenorhabdus spp. and Photorhabdus spp. Microbiol Rev 60: 21-43.
13. Forst S, Dowds B, Boemare N, Stackebrandt E (1997) Xenorhabdus and Photorhabdus spp.: bugs that kill bugs. Annu Rev Microbiol 51: 47-72.
14. Gatehouse J (2008) Biotechnological prospects for engineering insect-resistant plants. Plant Physiol 146: 881-887.
15. Gotz P, Boman A, Boman HG (1981) Interactions between insect immunity and an insect pathogenic nematode with symbiotic bacteria. Proc R Soc Lond B 212: 333-350.
16. Gupta GP, Birah A, Singh B, Mahapatro GK (2010) Methodology and composition of artificial diet for mass rearing of lepidopteran pests (in particular Helicoverpa armigera, Spodoptera litura and Earias vittella). Patent (IPA No 1618/DEL/2008) The Patent Office Journal, Part I, 16th, pp 15.
17. Hans M, Lars Z (2003) Chitin metabolism in insects: structure, function and regulation of chitin synthases and chitinases. J Exp Biol 206: 4393-4412.
18. Herbert EE, Goodrich-Blair H (2007) Friend and foe: the two faces of Xenorhabdus nematophila. Nat Rev Microbiol 5: 634-646.
19. Hofgen R, Willmitzer L (1988) Storage of competent cells for Agrobacterium transformation. Nucleic Acids Res 16: 9877.
20. Horsch RB, Fry JE, Hoffmann NL, Eichholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227: 1229-1231.
21. Joshi MC, Sharma A, Kant S, Birah A, Gupta GP, Khan SR, Bhatnagar R, Banerjee N (2008) An insecticidal GroEL Protein with chitin binding activity from Xenorhabdus nematophila. J Biol Chem 283: 28287-28296.
22. Khandelwal P, Banerjee BN (2003) Insecticidal activity associated with the outer membrane vesicles of Xenorhabdus nematophilus. Appl Environ Microbiol 69: 2032-2037.
23. Khandelwal P, Bhatnagar R, Choudhury D, Banerjee N (2004a) Characterization of a cytotoxic pilin subunit of Xenorhabdus nematophila. Biochem Biophys Res Commun 314: 943-949.
24. Khandelwal P, Choudhury D, Birah A, Reddy MK, Gupta GP, Banerjee N (2004b) Insecticidal pilin subunit from the insect pathogen Xenorhabdus nematophila. J Bacteriol 186: 6465-6476.
25. Liu D, Burton S, Glancy T, Li ZS, Hampton R, Meade T, Merlo DJ (2003) Insect resistance conferred by 283 kDa Photorhabdus luminescens protein TcdA in Arabidopsis thaliana. Nat Biotechnol 10: 1222-1228.
26. Morgan JAW, Sergeant M, Ellis D, Ousley M, Jerrett P (2001) Sequence analysis of insecticidal genes from Xenorhabdus nematophilus, PMF 1296. App Environ Microbiol 67: 2062-2096.
27. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissues cultures. Physiol Plant 15: 473-479.
28. Naranjo SE (2011) Impacts of Bt transgenic cotton on integrated pest management. J Agric Food Chem 59: 5842-5851.
29. Ranford JC, Henderson B (2002) Chaperonins in disease: mechanisms, models, and treatments. Mol Pathol 55: 209-213.
30. Reed W, Pawar CS (1982) Heliothis: a global problem. In: Proceedings of the International Workshop on Heliothis Management, Nov 15-20 1981. ICRISAT, Patancheru, pp 9-14.
31. Rogers SO, Bendich AJ (1994) Extraction of total cellular DNA from plants, algae and fungi. Plant Mol Biol Manual D1: 1-8.
32. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor, New York.
33. Sergeant M, Jarrett P, Ousley M, Morgan JAW (2003) Interactions of insecticidal toxin gene products from Xenorhabdus nematophilus PMFI296. App Environ Microbiol 69: 3344-3349.
34. Sharma H, Sharma K, Seetharama N, Ortiz R (2000) Prospects for transgenic resistance to insects in crop improvement. Elect J Biotechnol 3: 76-95.
35. Sharma HC, Sharma KK, Crouch JH (2004) Genetic transformation of crops for insect resistance: potential and limitations. Crit Rev Plant Sci 23: 47-72.
36. Shelton AM, Zhao JZ, Roush RT (2002) Economic, ecological, food safety, and social consequences of the deployment of Bt transgenic plants. Annu Rev Entomol 47: 845-881.
37. Sicard M, Brugirard-Ricaud K, Lanois A, Boemare NE, Bréhelin M, Givaudan A (2004) Stages of infection during the tripartite interaction between Xenorhabdus nematophila, its nematode vector, and insect hosts. Appl Environ Microbiol 70: 6473-6478.
38. Tabashnik BE, Gassmann AJ, Crowder DE, Carriere Y (2008) Insect resistance to Bt crops: evidence versus theory. Nature Biotechnol 26: 199-202.
39. Tabashnik BE, Unnithan GC, Masson L, Crowder DW, Li X, Carrière Y (2009) Asymmetrical cross-resistance between Bacillus thuringiensis toxins Cry1Ac and Cry2Ab in pink bollworm. Proc Natl Acad Sci USA 106: 11889-11894.
40. Tachibana MH, Hori N, Suzuki T, Uechi D, Kobayashi HI, Kaya HK (1996) Larvicidal activity of the symbiotic bacterium Xenorhabdus japonicum from the entomopathogenic nematode Steinernema kushidia against Anomala cuprae (coleopteran: Scarabaeidae). J Invertebr Pathol 68: 152-159.
41. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354.
42. United States Environmental Protection Agency (2009) Bacillus thuringiensis Cry1Ab delta-endotoxin protein and the genetic material necessary for its production (via elements of vector pZO1502) in event Bt11 Corn (OECD unique identifier: SYN-BTØ11-1)(006444) and Bacillus thuringiensisVip3Aa20 insecticidal protein and the genetic material necessary for its production (via elements of vector pNOV1300) in event MIR162 maize (OECD unique identifier: SYN-IR162-4)(006599) and modified Cry3A protein and the genetic material necessary for its production (via elements of vector pZM26) in event MIR604 corn (OECD unique identifier: SYN-IR6Ø4-5)(006509). Fact sheet 006599-006444-006509. Available at: http://www. epa. gov/opp00001/biopesticides/singredients/factsheets/factsheet_006599-006444. html. Accessed Feb 2 2010.
43. van Frankenhuyzen K, Nystrom C (2002) The Bacillus thuringiensis toxin specificity database. (http://www. glfc. forestry. ca/bacillus/BtSearch. cfm).
44. Waterfield NR, Bowen DJ, Fetherston JD, Perry RD, Ffrench Constant RH (2001) The tc genes of Photorhabdus: a growing family. Trends Microbiol 9: 185-191.
45. Whalon M, Mota-sanchez D, Hollongworth L, Duynslager L (2004) Arthopod pesticide resistance database. (Michigan State University, East Lansing, MI). Available at: http://www. pesticideresistance. org/search/12/0/41/0/. Accessed April 21 2009.
46. Yoshida N, Oeda K, Watanabe E (2001) Protein function: chaperonin turned insect toxin. Nature 411: 444.