Effect of a cholesterol-rich lipid environment on the enzymatic activity of reconstituted hyaluronan synthase

Biochemical and Biophysical Research Communications

Volumn 443, Issue 2, 2014, Pages 666-671

  Ontong, Pawared ^a   Hatada, Yasuyo ^b   Taniguchi, Shun'Ichiro ^b   Kakizaki, Ikuko ^c   Itano, Naoki ^a  

^a KYOTO SANGYO UNIVERSITY   (Japan)

^b SHINSHU UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^c HIROSAKI UNIVERSITY   (Japan)

Author keywords

Biosynthesis; Cholesterol; Hyaluronan; Lipid; Synthase

Indexed keywords

CHOLESTEROL; GLYCOSYLTRANSFERASE; HYALURONAN SYNTHASE; HYALURONIC ACID; LIPID; LIPOPROTEIN; LIPOSOME; MEMBRANE PROTEIN; METHYL BETA CYCLODEXTRIN; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLETHANOLAMINE; PHOSPHATIDYLSERINE; POLYHISTIDINE TAG; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; BIOSYNTHESIS; CELL MEMBRANE; CONTROLLED STUDY; ENZYME ACTIVITY; FIBROBLAST; GLYCOSAMINOGLYCAN METABOLISM; HUMAN; HUMAN CELL; LIPID COMPOSITION; MICROENVIRONMENT; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN ISOLATION; SOLUBILIZATION;

VERTEBRATA;

BIOSYNTHESIS; CHOLESTEROL; DIMYRISTOYL PC; DIMYRISTOYL PE; DIMYRISTOYL PS; DIOLEOYL PC; DIOLEOYL PE; DIOLEOYL PS; DIPALMITOYL PC; DIPALMITOYL PE; DIPALMITOYL PS; DISTEAROYL PC; DISTEAROYL PE; DISTEAROYL PS; DMPC; DMPE; DMPS; DOPC; DOPE; DOPS; DPPC; DPPE; DPPS; DSPC; DSPE; DSPS; HYALURONAN; LIPID; PC; PE; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLETHANOLAMINE; PHOSPHATIDYLSERINE; PS; SYNTHASE;

CELLS, CULTURED; CHOLESTEROL; ENZYME ACTIVATION; FIBROBLASTS; GLUCURONOSYLTRANSFERASE; HUMANS; HYALURONIC ACID; LIPID METABOLISM;

EID: 84892439945     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2013.12.028     Document Type: Article

References (21)

1. T.C. Laurent, and J.R.F. Fraser Hyaluronan FASEB J. 6 1992 2397 2404
2. P.H. Weigel, V.C. Hascall, and M. Tammi Hyaluronan synthases J. Biol. Chem. 272 1997 13997 14000
3. P.L. DeAngelis, J. Papaconstantinou, and P.H. Weigel Molecular cloning, identification, and sequence of the hyaluronan synthase gene from group A Streptococcus pyogenes J. Biol. Chem. 268 1993 19181 19184
4. N. Itano, and K. Kimata Mammalian hyaluronan synthases IUBMB Life 54 2002 195 199
5. V.L. Tlapak-Simmons, B.A. Baggenstoss, T. Clyne, and P.H. Weigel Purification and lipid dependence of the recombinant hyaluronan synthases from Streptococcus pyogenes and Streptococcus equisimilis J. Biol. Chem. 274 1999 4239 4245
6. P.H. Weigel, Z. Kyossev, and L.C. Torres Phospholipid dependence and liposome reconstitution of purified hyaluronan synthase J. Biol. Chem. 281 2006 36542 36551
7. D.A. Brown, and E. London Structure and function of sphingolipid- and cholesterol-rich membrane rafts J. Biol. Chem. 275 2000 17221 17224
8. K. Simons, and E. Ikonen Functional rafts in cell membranes Nature 387 1997 569 572
9. N. Itano, T. Sawai, M. Yoshida, P. Lenas, Y. Yamada, M. Imagawa, T. Shinomura, M. Hamaguchi, Y. Yoshida, Y. Ohnuki, S. Miyauchi, A.P. Spicer, J.A. McDonald, and K. Kimata Three isoforms of mammalian hyaluronan synthases have distinct enzymatic properties J. Biol. Chem. 274 1999 25085 25092
10. E.G. Bligh, and W.J. Dyer A rapid method of total lipid extraction and purification Can. J. Biochem. Physiol. 37 1959 911 917
11. W. Jiskoot, T. Teerlink, E.C. Beuvery, and D.J. Crommelin Preparation of liposomes via detergent removal from mixed micelles by dilution. The effect of bilayer composition and process parameters on liposome characteristics Pharm. Weekbl. Sci. 8 1986 259 265
12. S.W. Sakr, S. Potter-Perigo, M.G. Kinsella, P.Y. Johnson, K.R. Braun, Y. Goueffic, M.E. Rosenfeld, and T.N. Wight Hyaluronan accumulation is elevated in cultures of low density lipoprotein receptor-deficient cells and is altered by manipulation of cell cholesterol content J. Biol. Chem. 283 2008 36195 36204
13. A. Kultti, R. Karna, K. Rilla, P. Nurminen, E. Koli, K.M. Makkonen, J. Si, M.I. Tammi, and R.H. Tammi Methyl-beta-cyclodextrin suppresses hyaluronan synthesis by down-regulation of hyaluronan synthase 2 through inhibition of Akt J. Biol. Chem. 285 2010 22901 22910
14. E. Karousou, M. Kamiryo, S.S. Skandalis, A. Ruusala, T. Asteriou, A. Passi, H. Yamashita, U. Hellman, C.H. Heldin, and P. Heldin The activity of hyaluronan synthase 2 is regulated by dimerization and ubiquitination J. Biol. Chem. 285 2010 23647 23654
15. J.R. Silvius, D. del Giudice, and M. Lafleur Cholesterol at different bilayer concentrations can promote or antagonize lateral segregation of phospholipids of differing acyl chain length Biochemistry 35 1996 15198 15208
16. C. Bookstein, M.W. Musch, P.K. Dudeja, R.L. McSwine, Y. Xie, T.A. Brasitus, M.C. Rao, and E.B. Chang Inverse relationship between membrane lipid fluidity and activity of Na+/H+ exchangers, NHE1 and NHE3, in transfected fibroblasts J. Membr. Biol. 160 1997 183 192
17. S.L. Veatch From small fluctuations to large-scale phase separation: lateral organization in model membranes containing cholesterol Semin. Cell Dev. Biol. 18 2007 573 582
18. F.T. Presti, R.J. Pace, and S.I. Chan Cholesterol-phospholipid interaction in membranes. 2. Stoichiometry and molecular packing of cholesterol-rich domains Biochemistry 21 1982 3831 3835
19. A.H. Etemadi Membrane asymmetry. A survey and critical appraisal of the methodology. II. Methods for assessing the unequal distribution of lipids Biochim. Biophys. Acta 604 1980 423 475
20. V. Kiessling, J.M. Crane, and L.K. Tamm Transbilayer effects of raft-like lipid domains in asymmetric planar bilayers measured by single molecule tracking Biophys. J. 91 2006 3313 3326
21. J.L. Delaunay, M. Breton, G. Trugnan, and M. Maurice Differential solubilization of inner plasma membrane leaflet components by Lubrol WX and Triton X-100 Biochim. Biophys. Acta 1778 2008 105 112