Multi-centre evaluation of real-time multiplex PCR for detection of carbapenemase genes OXA-48, VIM, IMP, NDM and KPC

BMC Infectious Diseases

Volumn 14, Issue 1, 2014, Pages

  Van der Zee, Anneke ^a   Roorda, Lieuwe ^a   Bosman, Gerda ^a   Fluit, Ad C ^b   Hermans, Mirjam ^c   Smits, Paul H M ^d   van der Zanden, Adri G M ^e   te Witt, René ^f   Bruijnesteijn van Coppenraet, Lesla E S ^g   Cohen Stuart, James ^h   Ossewaarde, Jacobus M ^a,f  

^a Maasstad Hospital   (Netherlands)

^b UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

^c JEROEN BOSCH HOSPITAL   (Netherlands)

^d SLOTERVAART HOSPITAL   (Netherlands)

^e Laboratory for Medical Microbiology and Public Health   (Netherlands)

^f ERASMUS MEDICAL CENTER   (Netherlands)

^g ISALA HOSPITAL   (Netherlands)

^h Medical Centre Alkmaar   (Netherlands)

Author keywords

Carbapenemases; IMP; KPC; NDM; OXA 48; Real time multiplex PCR; VIM

Indexed keywords

CARBAPENEMASE; BACTERIAL PROTEIN; BETA LACTAMASE; CARBAPENEM DERIVATIVE;

ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM ISOLATION; GENETIC ANALYSIS; GENETIC IDENTIFICATION; GRAM NEGATIVE BACTERIUM; IMP GENE; KPC GENE; MULTIPLEX POLYMERASE CHAIN REACTION; NDM GENE; NONHUMAN; OXA 48 GENE; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; SENSITIVITY AND SPECIFICITY; VIM GENE; ANTIBIOTIC RESISTANCE; CLINICAL TRIAL; ENTEROBACTERIACEAE; EVALUATION STUDY; GENETICS; HUMAN; MULTICENTER STUDY;

BACTERIAL PROTEINS; BETA-LACTAMASES; CARBAPENEMS; DRUG RESISTANCE, BACTERIAL; ENTEROBACTERIACEAE; HUMANS; MULTIPLEX POLYMERASE CHAIN REACTION; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 84892397193     PISSN: None     EISSN: 14712334     Source Type: Journal    
DOI: 10.1186/1471-2334-14-27     Document Type: Article

References (15)

1. Cuzon G, Ouanich J, Gondret R, Naas T, Nordmann P. Outbreak of OXA-48-positive carbapenem-resistant Klebsiella pneumoniae isolates in France. Antimicrob Agents Chemother 2011, 55:2420-2423. 10.1128/AAC.01452-10, 3088266, 21343451.
2. Goren MG, Chmelnitsky I, Carmeli Y, Navon-Venezia S. Plasmid-encoded OXA-48 carbapenemase in Escherichia coli from Israel. J Antimicrob Chemother 2011, 66(3):672-673. 10.1093/jac/dkq467, 21177669.
3. Kitchel B, Rasheed JK, Patel JB, Srinivasan A, Navon-Venezia S, Carmeli Y, Brolund A, Giske CG. Molecular Epidemiology of KPC-Producing Klebsiella pneumoniae Isolates in the United States: Clonal Expansion of Multilocus Sequence Type 258. Antimicrob Agents Chemother 2009, 53(8):3365-3370. 10.1128/AAC.00126-09, 2715580, 19506063.
4. Girlich D, Poirel L, Nordmann P. Value of the modified hodge test for detection of emerging carbapenemases in Enterobacteriaceae. J Clin Microbiol 2012, 50:477-479. 10.1128/JCM.05247-11, 3264163, 22116154.
5. Giske CG, Gezelius L, Samuelsen T, Warner M, Sundsfjord A, Woodford N. A sensitive and specific phenotypic assay for detection of metallo-β-lactamases and KPC in Klebsiella pneumoniae with the use of meropenem disks supplemented with aminophenylboronic acid, dipicolinic acid and cloxacillin. Clin Microbiol Infect 2011, 17(4):552-556. 10.1111/j.1469-0691.2010.03294.x, 20597925.
6. Pasteran F, Mendez T, Guerriero L, Rapoport M, Corso A. Sensitive screening tests for suspected class A carbapenemase production in species of Enterobacteriaceae. J Clin Microbiol 2009, 47:1631-1639. 10.1128/JCM.00130-09, 2691115, 19386850.
7. Tsakris A, Poulou A, Pournaras S, Voulgari E, Vrioni G, Themeli-Digalaki K, Petropoulou D, Sofianou D. A simple phenotypic method for the differentiation of metallo-beta-lactamases and class A KPC carbapenemases in Enterobacteriaceae clinical isolates. J Antimicrob Chemother 2010, 65(8):664-671.
8. Cohen Stuart J, Leverstein-Van Hall MA. on behalf of members of the Dutch Working Party on the Detection of Highly Resistant Microorganisms Guideline for phenotypic screening and confirmation of carbapenemases in Enterobacteriaceae. Int J Antimicrob Agents 2010, 36:205-210. 10.1016/j.ijantimicag.2010.05.014, 20598859, on behalf of members of the Dutch Working Party on the Detection of Highly Resistant Microorganisms.
9. Van Hannen EJ. Nieuwegein, The Netherlands: St. Antonius Hospital, e.vanhannen@antoniusziekenhuis.nl. Personal communication.
10. Voets GM, Fluit AC, Scharringa J, Cohen Stuart J, Leverstein-van Hall MA. A set of multiplex PCRs for genotypic detection of extended-spectrum beta-lactamases, carbapenemases, plasmid-mediated AmpC 1-lactamases and OXA-lactamases. Int J Antimicrobial Agents 2011, 37:356-359.
11. Chen L, Mediavilla JR, Endimiani A, Rosenthal ME, Zhao Y, Bonomo RA, Kreiswirth BN. Multiplex Real-Time PCR Assay for Detection and Classification of Klebsiella pneumoniae Carbapenemase Gene (blaKPC) Variants. J Clin Microbiol 2011, 49(2):579-585. 10.1128/JCM.01588-10, 3043520, 21123529.
12. Cole JM, Schuetz AN, Hill CE, Nolte FS. Development and Evaluation of a Real-Time PCR Assay for Detection of Klebsiella pneumoniae Carbapenemase Genes. J Clin Microbiol 2009, 47(2):322-326. 10.1128/JCM.01550-08, 2643690, 19036932.
13. Naas T, Ergani A, Carrër A, Nordmann P. Real-Time PCR for Detection of NDM-1 Carbapenemase Genes from Spiked Stool Samples. Antimicrob Agents Chemother 2011, 55(9):4038-4043. 10.1128/AAC.01734-10, 3165338, 21690281.
14. Poirel L, Walsh TR, Cuvillier V, Nordmann P. Multiplex PCR for detection of acquired carbapenemase genes. Diagn Microbiol Infect Dis 2011, 70:119-123. 10.1016/j.diagmicrobio.2010.12.002, 21398074.
15. Van der Bij AK, Mol M, Van Westreenen M, Goessens WHF, Pitout JDD. The laboratory diagnosis of Pseudomonas aeruginosa that produce metallo- beta -lactamases in a Dutch tertiary care centre. Scand J Infect Dis 2011, 43(8):596-602. 10.3109/00365548.2011.574148, 21506891.