Leaf extract of Azadirachta indica (neem): A potential antibiofilm agent for Pseudomonas aeruginosa

Pathogens and Disease

Volumn 69, Issue 1, 2013, Pages 62-65

  Harjai, Kusum ^a   Bala, Anju ^a   Gupta, Ravi K ^a   Sharma, Radhika ^a  

^a PANJAB UNIVERSITY   (India)

Author keywords

Azadirachta indica (neem); Biofilms; Pseudomonas aeruginosa; Scanning electron microscope

Indexed keywords

ALGINIC ACID; ANTIBIOTIC AGENT; AZADIRACHTA INDICA EXTRACT; EXOPOLYSACCHARIDE; ADHESIN; ANTIINFECTIVE AGENT; PLANT EXTRACT;

ARTICLE; BACTERIAL CELL; BACTERIAL GROWTH; BACTERIOSTASIS; BIOFILM; CELL ADHESION; CELL SURFACE; CELLS; CONTROLLED STUDY; GROWTH INHIBITION; HUMAN; HUMAN CELL; HYDROPHOBICITY; OPTICAL DENSITY; PLANT LEAF; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; AZADIRACHTA INDICA; BACTERIUM ADHERENCE; CHEMISTRY; DRUG EFFECTS; ISOLATION AND PURIFICATION; METABOLISM; PHYSIOLOGY;

ADHESINS, BACTERIAL; ANTI-BACTERIAL AGENTS; AZADIRACHTA; BACTERIAL ADHESION; BIOFILMS; PLANT EXTRACTS; PLANT LEAVES; PSEUDOMONAS AERUGINOSA;

EID: 84892386180     PISSN: None     EISSN: 2049632X     Source Type: Journal    
DOI: 10.1111/2049-632X.12050     Document Type: Article

References (28)

1. Aarati N, Ranganth NN, Soumya BG, Kishore B & Mithun K (2011) Evaluation of antibacterial and anticandidial efficacy of aqueous and alcoholic extract of neem (Azadirachta indica)- an in vitro study. IJRAP 2: 230-235.
2. Agbenin ON & Marley PS (2006) In-vitro assay of some plant extracts against Fusarium oxysporum f. sp. Lycopersici causal agent of tomato wilt. J Plant Protec Res 46: 215-220.
3. Ahmad I, Mehmood Z & Mohammad F (1998) Screening of some Indian medicinal plants for their antimicrobial properties. J Ethnopharmacol 62: 183-193.
4. Bedi MS, Verma V & Chhibber S (2009) Amoxicillin and specific bacteriophage can be used together for eradication of biofilm of Klebsiella pneumoniae B5055. World J Microbiol Biotechnol 25: 1145-1151.
5. Bjarnsholt T, Jensen PO, Burmolle M, Hentzer M, Haagensen JAJ, Hougen HP, Calum H, Madsen KG & Moser C (2005a) Pseudomonas aeruginosa tolerance to tobramycin, hydrogen peroxide and polymorphonuclear leukocytes is quorum-sensing dependent. Microbiology 151: 373-383.
6. Bjarnsholt T, Jensen PO, Jakobsen TH, Phipps R, Nielsen AK, Rybtke MT, Tolker-Nielsen T, Givskov M, Hoiby N & Ciofu O (2010) Quorum sensing and virulence of Pseudomonas aeruginosa during lung infection of cystic fibrosis patients. PLoS ONE 5: e10115.
7. Daifuku R & Stamm WE (1986) Bacterial adherence to bladder uroepithelial cells in catheter-associated urinary tract infection. New Engl J Med 314: 1208-1213.
8. Dubois M, Gilles KA, Hamilton JK, Rebers PA & Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28: 350-356.
9. Ehrlich GD, Veeh R, Wang X, Costerton JW, Hayes JD, Hu FZ, Daigle BJ, Ehrlich MD & Post JC (2002) Mucosal biofilm formation on middle-ear mucosa in the chinchilla model of otitis media. JAMA 287: 1710-1715.
10. El Astal ZY, Aera A & Aam A (2005) Antimicrobial activity of some medicinal plant extracts in Palestine. Pak J Med Sci 21: 187.
11. Ganguli S (2002) Neem: a therapeutic for all seasons. Curr Sci 82: 1304.
12. Gilbert P, Allison DG & McBain AJ (2002) Biofilms in vitro and in vivo: do singular mechanisms imply cross resistance? J App Microbiol 92: 98S-110S.
13. Harrison JJ, Ceri H, Stremick C & Turner RJ (2004) Differences in biofilm and planktonic cell mediated reduction of metalloid oxyanions. FEMS Microbiol Lett 235: 357-362.
14. Ito A, Toniuchi A, May T, Kawata K & Okabe S (2009) Increased antibiotic resistance of Escherichia coli in mature biofilms. Appl Environ Microbiol 75: 4093-4100.
15. Kallel H, Mahjoubi F, Dammak H, Bahloul M, Hamida CB, Chelly H, Rekik N, Hammami A & Bouaziz M (2008) Correlation between antibiotic use and changes in susceptibility patterns of Pseudomonas aeruginosa in a medical-surgical intensive care unit. Indian J Crit Care Med 12: 18-23.
16. Kustos T, Kustos I, Kilar F, Rappai G & Kocsis B (2003) Effect of antibiotics on cell surface hydrophobicity of bacteria causing orthopedic wound infections. Chemotherapy 49: 237-242.
17. Maragathavalli S, Brindha S, Kaviyarasi NS, Annadurai B & Gangwar SK (2012) Antimicrobial activity in leaf extract of Neem (Azadirachta Indica Linn.). Int J Sci Nat 3: 110-113.
18. Mathee K, Ciofu O, Sternberg C, Lindum PW, Campbell JIA, Jensen P, Johnsen AH, Givskov M & Ohman DE (1999) Mucoid conversion of Pseudomonas aeruginosa by hydrogen peroxide: a mechanism for virulence activation in the cystic fibrosis lung. Microbiology 145: 1349-1357.
19. Mittal R, Sharma S, Chhibber S & Harjai K (2006) Effect of macrophage secretory products on elaboration of virulence factors by planktonic and biofilm cells of Pseudomonas aeruginosa. Comp Immunol Microbiol Infect Dis 29: 12-26.
20. Nickel JC, Ruseska I, Wright JB & Costerton JW (1985) Tobramycin resistance of Pseudomonas aeruginosa cells growing as a biofilm on urinary catheter material. Antimicrob Agents Chemother 27: 619-624.
21. Obuekwe CO, Al-Jadi ZK & Al-Saleh ES (2008) Comparative hydrocarbon utilization by hydrophobic and hydrophilic variants of Pseudomonas aeruginosa. J Appl Microbiol 105: 876-1887.
22. Pai MR, Acharya LD & Udupa N (2004) Evaluation of antiplaque activity of Azadirachta indica leaf extract gel-a 6- week clinical study. J Ethnopharmacol 90: 99-1039.
23. Polaquini SR, Svidzinski TI, Kemmelmeier C & Gasparetto A (2006) Effect of aqueous extract from neem (Azadirachta indica A. juss) on hydrophobicity, biofilm formation an adhesion in composite resin by Candida albicans. Arch Oral Biol 51: 482-490.
24. Rosenberg M, Gutnick D & Rosenberg E (1980) Adherence of bacteria to hydrocarbons. A simple method for measuring cell surface hydrophobicity. FEMS Microbiol Lett 9: 29-33.
25. Sharma S, Madhur BS, Singh R & Sharma BK (1987) Effect of contraceptive on adhesion of E. coli to uroepithelial cells. J Infect Dis 156: 490-496.
26. Stoodley P, Sauer K, Davies DG & Costerton JW (2002) Biofilms as complex differentiated communities. Annu Rev Microbiol 56: 187-209.
27. Sutherland I (2001) Biofilm exopolysaccharides: a strong and sticky framework. Microbiology 147: 3-9.
28. Toutain CM, Caiazza NC & O'Toole GA (2004) Molecular basis of biofilm development by pseudomonads. Microbial Biofilms (Ghannoum M & O'Toole GA, eds), pp. 43-63. ASM Press, Washington, DC.