Modification of β-oxidation pathway in Ralstonia eutropha for production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from soybean oil

Journal of Bioscience and Bioengineering

Volumn 117, Issue 2, 2014, Pages 184-190

  Insomphun, Chayatip ^a   Mifune, Jun ^a   Orita, Izumi ^a   Numata, Keiji ^b   Nakamura, Satoshi ^a   Fukui, Toshiaki ^a  

^a TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^b RIKEN   (Japan)

Author keywords

Oxidation; Bioplastic; Polyhydroxyalkanoate; Ralstonia eutropha; Vegetable oil

Indexed keywords

BIOPLASTICS; ENGINEERED STRAINS; GENE DISRUPTIONS; HYDROXYBUTYRATE; OXIDATION PATHWAY; POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYHEXANOATE); POLY-HYDROXYALKANOATE; RALSTONIA EUTROPHA;

BACTERIA; BIOCHEMISTRY; BIOSYNTHESIS; CELL GROWTH; GENES; GROWTH KINETICS; REINFORCED PLASTICS; SOYBEAN OIL; VEGETABLE OILS;

OXIDATION;

3 HYDROXYACYL COENZYME A DEHYDROGENASE; ENOYL COENZYME A HYDRATASE; HEXANOIC ACID DERIVATIVE; POLY(3 HYDROXYBUTYRATE CO 3 HYDROXYHEXANOATE); SOYBEAN OIL; UNCLASSIFIED DRUG;

AEROMONAS PUNCTATA; ARTICLE; BACTERIAL STRAIN; CELL GROWTH; CHROMOSOME 1; CHROMOSOME 2; CUPRIAVIDUS NECATOR; ENZYME MODIFICATION; ENZYME SYNTHESIS; FATTY ACID OXIDATION; GENE CLUSTER; GENE DELETION; GENE DISRUPTION; NONHUMAN;

AEROMONAS PUNCTATA; BACTERIA (MICROORGANISMS); CUPRIAVIDUS NECATOR; GLYCINE MAX;

BIOPLASTIC; POLYHYDROXYALKANOATE; RALSTONIA EUTROPHA; VEGETABLE OIL; Β-OXIDATION;

3-HYDROXYBUTYRIC ACID; ACYLTRANSFERASES; CAPROATES; CHROMOSOMES, BACTERIAL; CUPRIAVIDUS NECATOR; ENOYL-COA HYDRATASE; FRUCTOSE; GENE DELETION; GENES, BACTERIAL; OXIDATION-REDUCTION; SOYBEAN OIL;

EID: 84892366144     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2013.07.016     Document Type: Article

References (38)

1. Madison L.L., Huisman G.W. Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic. Microbiol. Mol. Biol. Rev. 1999, 63:21-53.
2. Rehm B.H. Bacterial polymers: biosynthesis, modifications and applications. Nat. Rev. Microbiol. 2010, 8:578-592.
3. Steinbüchel A. PHB and other polyhydroxyalkanoic acids. Biotechnology: products of primary metabolism 2008, vol. 6:403-464. Wiley-VCH, Weinheim. 2nd ed.
4. Hein S., Paletta J.R., Steinbüchel A. Cloning, characterization and comparison of the Pseudomonas mendocina polyhydroxyalkanoate synthases PhaC1 and PhaC2. Appl. Microbiol. Biotechnol. 2002, 58:229-236.
5. Huisman G.W., Wonink E., Meima R., Kazemier B., Terpstra P., Witholt B. Metabolism of poly(3-hydroxyalkanoates) (PHAs) by Pseudomonas oleovorans. Identification and sequences of genes and function of the encoded proteins in the synthesis and degradation of PHA. J.Biol. Chem. 1991, 266:2191-2198.
6. Kato M., Bao H.J., Kang C.-K., Fukui T., Doi Y. Production of a novel copolyester of 3-hydroxybutyric acid and medium-chain-length 3-hydroxyalkanoic acids by Pseudomonas sp. 61-3 from sugars. Appl. Microbiol. Biotechnol. 1996, 45:363-370.
7. Matsusaki H., Manji S., Taguchi K., Kato M., Fukui T., Doi Y. Cloning and molecular analysis of the poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyalkanoate) biosynthesis genes in Pseudomonas sp. strain 61-3. J.Bacteriol. 1998, 180:6459-6467.
8. Timm A., Steinbuchel A. Cloning and molecular analysis of the poly(3-hydroxyalkanoic acid) gene locus of Pseudomonas aeruginosa PAO1. Eur. J. Biochem. 1992, 209:15-30.
9. Doi Y., Kitamura S., Abe H. Microbial synthesis and characterization of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate). Macromolecules 1995, 28:4822-4828.
10. Chee J.Y., Lau N.S., Samian M.R., Tsuge T., Sudesh K. Expression of Aeromonas caviae polyhydroxyalkanoate synthase gene in Burkholderia sp. USM (JCM15050) enables the biosynthesis of SCL-MCL PHA from palm oil products. J.Appl. Microbiol. 2012, 112:45-54.
11. Fukui T., Kichise T., Yoshida Y., Doi Y. Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyheptanoate) terpolymers by recombinant Alcaligenes eutrophus. Biotechnol. Lett. 1997, 19:1093-1097.
12. Fukui T., Shiomi N., Doi Y. Expression and characterization of (R)-specific enoyl coenzyme A hydratase involved in polyhydroxyalkanoate biosynthesis by Aeromonas caviae. J.Bacteriol. 1998, 180:667-673.
13. Fujita Y., Matsuoka H., Hirooka K. Regulation of fatty acid metabolism in bacteria. Mol. Microbiol. 2007, 66:829-839.
14. DiRusso C.C. Primary sequence of the Escherichia coli fadBA operon, encoding the fatty acid-oxidizing multienzyme complex, indicates a high degree of homology to eucaryotic enzymes. J.Bacteriol. 1990, 172:6459-6468.
15. Fiedler S., Steinbuchel A., Rehm B.H. The role of the fatty acid β-oxidation multienzyme complex from Pseudomonas oleovorans in polyhydroxyalkanoate biosynthesis: molecular characterization of the fadBA operon from P.oleovorans and of the enoyl-CoA hydratase genes phaJ from P.oleovorans and Pseudomonas putida. Arch. Microbiol. 2002, 178:149-160.
16. Kunau W.H., Dommes V., Schulz H. β-Oxidation of fatty acids in mitochondria, peroxisomes, and bacteria: a century of continued progress. Prog. Lipid Res. 1995, 34:267-342.
17. Yang S.-Y., Elzinga M. Association of both enoyl coenzyme A hydratase and 3-hydroxyacyl coenzyme A epimerase with an active site in the amino-terminal domain of the multifunctional fatty acid oxidation protein from Escherichia coli. J.Biol. Chem. 1993, 268:6588-6592.
18. Kawashima Y., Cheng W., Mifune J., Orita I., Nakamura S., Fukui T. Characterization and functional analyses of R-specific enoyl coenzyme A hydratases in polyhydroxyalkanoate-producing Ralstonia eutropha. Appl. Environ. Microbiol. 2012, 78:493-502.
19. Tsuge T., Taguchi K., Seiichi T., Doi Y. Molecular characterization and properties of (R)-specific enoyl-CoA hydratases from Pseudomonas aeruginosa: metabolic tools for synthesis of polyhydroxyalkanoates via fatty acid β-oxidation. Int. J. Biol. Macromol. 2003, 31:195-205.
20. Nomura C.T., Taguchi K., Gan Z., Kuwabara K., Tanaka T., Takase K., Doi Y. Expression of 3-ketoacyl-acyl carrier protein reductase (fabG) genes enhances production of polyhydroxyalkanoate copolymer from glucose in recombinant Escherichia coli JM109. Appl. Environ. Microbiol. 2005, 71:4297-4306.
21. Ren Q., Sierro N., Witholt B., Kessler B. FabG, an NADPH-dependent 3-ketoacyl reductase of Pseudomonas aeruginosa, provides precursors for medium-chain-length poly-3-hydroxyalkanoate biosynthesis in Escherichia coli. J.Bacteriol. 2000, 182:2978-2981.
22. Taguchi K., Aoyagi Y., Matsusaki H., Fukui T., Doi Y. Co-expression of 3-ketoacyl-ACP reductase and polyhydroxyalkanoate synthase genes induces PHA production in Escherichia coli HB101 strain. FEMS Microbiol. Lett. 1999, 176:183-190.
23. Escapa I.F., Morales V., Martino V.P., Pollet E., Averous L., Garcia J.L., Prieto M.A. Disruption of β-oxidation pathway in Pseudomonas putida KT2442 to produce new functionalized PHAs with thioester groups. Appl. Microbiol. Biotechnol. 2011, 89:1583-1598.
24. Ouyang S.P., Luo R.C., Chen S.S., Liu Q., Chung A., Wu Q., Chen G.Q. Production of polyhydroxyalkanoates with high 3-hydroxydodecanoate monomer content by fadB and fadA knockout mutant of Pseudomonas putida KT2442. Biomacromolecules 2007, 8:2504-2511.
25. Qi Q., Steinbuchel A., Rehm B.H. Metabolic routing towards polyhydroxyalkanoic acid synthesis in recombinant Escherichia coli (fadR): inhibition of fatty acid β-oxidation by acrylic acid. FEMS Microbiol. Lett. 1998, 167:89-94.
26. Tsuge T., Watanabe S., Shimada D., Abe H., Doi Y., Taguchi S. Combination of N149S and D171G mutations in Aeromonas caviae polyhydroxyalkanoate synthase and impact on polyhydroxyalkanoate biosynthesis. FEMS Microbiol. Lett. 2007, 277:217-222.
27. Mifune J., Nakamura S., Fukui T. Engineering of pha operon on Cupriavidus necator chromosome for efficient biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from vegetable oil. Polym. Degrad. Stab. 2010, 95:1305-1312.
28. Budde C.F., Riedel S.L., Willis L.B., Rha C., Sinskey A.J. Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil by engineered Ralstonia eutropha strains. Appl. Environ. Microbiol. 2011, 77:2847-2854.
29. Brigham C.J., Budde C.F., Holder J.W., Zeng Q., Mahan A.E., Rha C., Sinskey A.J. Elucidation of β-oxidation pathways in Ralstonia eutropha H16 by examination of global gene expression. J.Bacteriol. 2010, 192:5454-5464.
30. Simon R., Priefer U., Pühler A. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Bio/Technology 1983, 1:784-791.
31. Schafer A., Tauch A., Jager W., Kalinowski J., Thierbach G., Puhler A. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 1994, 145:69-73.
32. Mifune J., Nakamura S., Fukui T. Targeted engineering of Cupriavidus necator chromosome for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from vegetable oil. Can. J. Chem. 2008, 86:621-627.
33. Kahar P., Tsuge T., Taguchi K., Doi Y. High yield production of polyhydroxyalkanoates from soybean oil by Ralstonia eutropha and its recombinant strain. Polym. Degrad. Stab. 2004, 83:79-86.
34. Ryu H.W., Hahn S.K., Chang Y.K., Chang H.N. Production of poly(3-hydroxybutyrate) by high cell density fed-batch culture of Alcaligenes eutrophus with phosphate limitation. Biotechnol. Bioeng. 1997, 55:28-32.
35. Green P.R., Kemper J., Schechtman L., Guo L., Satkowski M., Fiedler S., Steinbuchel A., Rehm B.H. Formation of short chain length/medium chain length polyhydroxyalkanoate copolymers by fatty acid β-oxidation inhibited Ralstonia eutropha. Biomacromolecules 2002, 3:208-213.
36. Liu W., Chen G.-Q. Production and characterization of medium-chain-length polyhydroxyalkanoate with high 3-hydroxytetradecanoate monomer content by fadB and fadA knockout mutant of Pseudomonas putida KT2442. Appl. Microbiol. Biotechnol. 2007, 76:1153-1159.
37. Park S.J., Choi J., Lee S.Y. Engineering of Escherichia coli fatty acid metabolism for the production of polyhydroxyalkanoates. Enzyme Microb. Technol. 2005, 36:579-588.
38. Campbell J.W., Morgan-Kiss R.M., Cronan J.E. Anew Escherichia coli metabolic competency: growth on fatty acids by a novel anaerobic β-oxidation pathway. Mol. Microbiol. 2003, 47:793-805.