Porphyromonas gingivalis C-terminal signal peptidase PG0026 and HagA interact with outer membrane protein PG27/LptO

Molecular Oral Microbiology

Volumn 29, Issue 1, 2014, Pages 32-44

  Saiki, K ^a   Konishi, K ^a  

^a NIPPON DENTAL UNIVERSITY   (Japan)

Author keywords

C terminal domain; Gingipain; Porphyromonas; Protein secretion

Indexed keywords

HAGA PROTEIN; HISTIDINE; OUTER MEMBRANE PROTEIN; OUTER MEMBRANE PROTEIN PG27; PG0026 PROTEIN; SIGNAL PEPTIDASE; UNCLASSIFIED DRUG; ADHESIN; ARGINGIPAIN, PORPHYROMONAS GINGIVALIS; BACTERIAL PROTEIN; CYSTEINE PROTEINASE; HEMAGGLUTININ A, PORPHYROMONAS GINGIVALIS; LECTIN; MEMBRANE PROTEIN; PROTEIN BINDING; SERINE PROTEINASE; TYPE I SIGNAL PEPTIDASE;

ARTICLE; CARBOXY TERMINAL SEQUENCE; GENE MUTATION; MASS SPECTROMETRY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PORPHYROMONAS GINGIVALIS; PROTEIN CROSS LINKING; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; AMINO ACID SEQUENCE; GENETICS; METABOLISM; NATIVE POLYACRYLAMIDE GEL ELECTROPHORESIS; PHYSIOLOGY;

ADHESINS, BACTERIAL; AMINO ACID SEQUENCE; BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL PROTEINS; CYSTEINE ENDOPEPTIDASES; LECTINS; MASS SPECTROMETRY; MEMBRANE PROTEINS; NATIVE POLYACRYLAMIDE GEL ELECTROPHORESIS; PORPHYROMONAS GINGIVALIS; PROTEIN BINDING; SERINE ENDOPEPTIDASES;

EID: 84891929978     PISSN: 20411006     EISSN: 20411014     Source Type: Journal    
DOI: 10.1111/omi.12043     Document Type: Article

References (33)

1. Chen, Y.-Y., Peng, B., Yang, Q. et al. (2011) The outer membrane protein LptO is essential for the O-deacylation of LPS and the co-ordinated secretion and attachment of A-LPS and CTD proteins in Porphyromonas gingivalis. Mol Microbiol 79: 1380-1401.
2. Christersson, L.A., Fransson, C.L., Dunford, R.G. and Zambon, J.J. (1992) Subgingival distribution of periodontal pathogenic microorganism in adult periodontitis. J Periodontol 63: 418-425.
3. Curtis, M.A., Kuramitsu, H.K., Lantz, M. et al. (1999) Molecular genetics and nomenclature of proteases of Porphyromonas gingivalis. J Periodontal Res 34: 464-472.
4. Curtis, M.A., Opoku, J.A., Rangarajan, M. et al. (2002) Attenuation of the virulence of Porphyromonas gingivalis by using a specific synthetic Kgp protease inhibitor. Infect Immun 70: 6968-6975.
5. Filloux, A., Hachani, A. and Blebes, S. (2008) The bacterial type VI secretion machine: yet another player for protein transport across membranes. Microbiology 154: 1570-1583.
6. Glew, M.D., Veith, P.D., Peng, B. et al. (2012) PG0026 is the C-terminal signal peptidase of a novel secretion system of Porphyromonas gingivalis. J Biol Chem 287: 24605-24617.
7. Han, N., Whitlock, J. and Progulske-Fox, A. (1996) The hemagglutinin gene A (hagA) of Porphyromonas gingivalis 381 contains four large, contiguous, direct repeats. Infect Immun 64: 4000-4007.
8. Haurat, M.F., Aduse-Opoku, J., Rangarajan, M. et al. (2011) Selective sorting of cargo proteins into bacterial membrane vesicles. J Biol Chem 286: 1269-1276.
9. Ishiguro, I., Saiki, K. and Konishi, K. (2009) PG27 is a novel membrane protein essential for a Porphyromonas gingivalis protease secretion system. FEMS Microbiol Lett 292: 261-267.
10. Ishiguro, I., Saiki, K. and Konishi, K. (2011) Analysis of Porphyromonas gingivalis PG27 by deletion and intragenic suppressor mutation analyses. Mol Oral Microbiol 26: 321-335.
11. Knowles, T.J., Scott-Tucker, A., Overduin, M. and Henderson, I.R. (2009) Membrane protein architects: the role of the Bam complex in outer membrane protein assembly. Nat Rev Microbiol 7: 206-214.
12. Kumagai, Y., Yajima, A. and Konishi, K. (2003) Peptidase activity of dipeptidyl aminopeptidase IV produced by Porphyromonas gingivalis is important but not sufficient for virulence. Microbiol Immun 47: 735-743.
13. Merril, C.R. (1990) Gel-staining techniques. In: Deutscher, M.P. ed. Methods in Enzymology: Guide to Protein Purification, Vol. 182. San Diego, CA: Academic Press Inc., pp. 477-488.
14. Naito, M., Sakai, E., Shi, Y. et al. (2006) Porphyromonas gingivalis-induced platelet aggregation in plasma depends on Hgp44 adhesin but not Rgp proteinase. Mol Microbiol 59: 152-167.
15. Naito, M., Hirakawa, H., Yamashita, A. et al. (2008) Determination of the genome sequence of Porphyromonas gingivalis strain ATCC 33277 and genome comparison with strain W83 revealed extensive genomic rearrangements in P. gingivalis. DNA Res 15: 215-225.
16. Nakayama, K. (1997) Domain-specific rearrangement between the two Arg-gingipain-encoding genes in Porphyromonas gingivalis: possible involvement of nonreciprocal recombination. Microbiol Immunol 41: 185-196.
17. Nelson, K.E., Fleischmann, R.D., DeBoy, R.T. et al. (2003) Complete genome sequence of the oral pathogenic bacterium Porphyromonas gingivalis strain W83. J Bacteriol 185: 5591-5601.
18. Oda, H., Saiki, K., Numabe, Y. and Konishi, K. (2007) Effect of γ-immunoglobulin on the asaccharolytic growth of Porphyromonas gingivalis. J Periodont Res 42: 438-442.
19. Saiki, K. and Konishi, K. (2007) Identification of a Porphyromonas gingivalis novel protein Sov required for the secretion of gingipains. Microbiol Immunol 51: 483-491.
20. Saiki, K. and Konishi, K. (2010a) The role of Sov protein in the secretion of gingipain protease virulence factors of Porphyromonas gingivalis. FEMS Microbiol Lett 302: 166-174.
21. Saiki, K. and Konishi, K. (2010b) Identification of a novel Porphyromonas gingivalis outer membrane protein, PG534, required for the production of active gingipains. FEMS Microbiol Lett 310: 168-174.
22. Saiki, K. and Konishi, K. (2012) Strategies for targeting the gingipain secretion system of Porphyromonas gingivalis. J Oral Biosci 54: 155-159.
23. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
24. Sato, K., Sakai, E., Veith, P.D. et al. (2005) Identification of a new membrane-associated protein that influences transport/maturation gingipains and adhesins of Porphyromonas gingivalis. J Biol Chem 280: 8668-8677.
25. Sato, K., Naito, M., Yukitake, H. et al. (2010) A protein secretion system linked to bacteroidete gliding motility and pathogenesis. Proc Natl Acad Sci U S A 107: 276-281.
26. Seers, C.A., Slakeski, N., Veith, P.D. et al. (2006) The RgpB C-terminal domain has a role in attachment of RgpB to the outer membrane and belongs to a novel C-terminal-domain family found in Porphyromonas gingivalis. J Bacteriol 188: 6376-6386.
27. Shoji, M., Sato, K., Yukitake, H. et al. (2011) Por secretion system-dependent secretion and glycosylation of Porphyromonas gingivalis hemin-binding protein 35. PLoS ONE 6: e21372.
28. Slakeski, N., Seers, C.A., Ng, K. et al. (2011) C-terminal domain residues important for secretion and attachment of RgpB in Porphyromonas gingivalis. J Bacteriol 193: 132-142.
29. Socransky, S.S. and Haffajee, A.D. (1992) The bacterial etiology of destructive periodontal disease: current concepts. J Periodontol 63: 322-331.
30. Sztukowska, M., Sroka, A., Bugno, M. et al. (2004) The C-terminal domains of the gingipain K polyprotein are necessary for assembly of the active enzyme and expression of associated activities. Molec Microbiol 54: 1393-1408.
31. Vanterpool, E., Roy, F., Sandberg, L. and Fletcher, H.M. (2005) Altered gingipain maturation in vimA- and vimE-defective isogenic mutants of Porphyromonas gingivalis. Infect Immun 73: 1357-1366.
32. Watanabe, T., Maruyama, F., Nozawa, T. et al. (2011) Complete genome sequence of the bacterium Porphyromonas gingivalis TDC60, which causes periodontal disease. J Bacteriol 193: 4259-4260.
33. Zhou, X.-Y., Gao, J.-L., Hunter, N., Potempa, J. and Nguyen, K.-A. (2013) Sequence-independent processing site of the C-terminal domain (CTD) influences maturation of the RgpB protease from Porphyromonas gingivalis. Mol Microbiol 89: 903-917.