A map to more reliable RT-qPCR results using MIQE guidelines

American Laboratory

Volumn 45, Issue 10, 2013, Pages 18-21

  Taylor, Sean ^a  

^a Bio Rad Laboratories (Canada) Ltd   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

DIFFERENT TREATMENTS; ENVIRONMENTAL FACTORS; MINIMUM INFORMATION; OPTIMAL PERFORMANCE; QUANTITATIVE POLYMERASE CHAIN REACTION; QUANTITATIVE REAL TIME PCR; REVERSE TRANSCRIPTION; SCIENTIFIC COMMUNITY;

BIOINFORMATICS; GENE EXPRESSION;

POLYMERASE CHAIN REACTION;

EID: 84891845198     PISSN: 00447749     EISSN: None     Source Type: Trade Journal    
DOI: None     Document Type: Article

References (21)

1. Williams, S.C.P. How reliable is real-time PCR? Biotechniques 2012.
2. Bustin, S.A.; Benes, V. et al.The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin. Chem. 2009, 55,611-22.
3. Bustin, S.A.; Vandesompele, J. et al. Standardization of qPCR and RT-qPCR. Gen. Eng. Biotechnol. News 2009,29(14), 1 -3.
4. Bustin, S.A. Why the need for qPCR publication guidelines? The case for MIQE. Methods 2010,50(4), 217-26.
5. Taylor, S.; Wakem, M. et al. A practical approach to RT-qPCR - publishing data according to the MIQE guidelines. Methods 2010,50(4), S1-5.
6. De Keyser, E.; Desmet, L. et al. How to perform RT-qPCR accurately in plant species? A case study on flower colour gene expression in an azalea (Rhododendron simsii hybrids) mapping population. BMC Mol. Biol. Jun 24, 2013, 74(1), 13.
7. Dooms, M.; Chango, A. et al. Improving biological relevancy of transcriptional biomarkers experiments by applying the MIQE guidelines to pre-clinical and clinical trials. Methods Jan 2013,59(1), 147-53.
8. Lanoix, D.; Lacasse, A.A. et al. Quantitative PCR pitfalls: the case of the human placenta. Mol. Biotechnol. Apr 17,2012.
9. Taylor, S.; Buchanan, M. et al. A MIQE case study - effect of RNA sample quality and reference gene stability on gene expression data. Bio-Rad Laboratories Tech Note 6245, Dec 2011,1 -6.
10. Google Scholar search for "qPCR" after 2010 related to total citations for the MIQE Guidelines paper (see Ref. 2).
11. Bohlenius, H.; Eriksson, S. et al. Retraction - the mRNA of the Arabi-dopsis gene FT moves from leaf to shoot apex and induces flowering. Science 2007,316(5823), 367. (Pubitemid 46651456)
12. Editors of Lancet. Retraction - ileal-lymphoid-nodular hyperplasia, nonspecific colitis, and pervasive developmental disorder in children. Lancet 2010,375(9713), 445.
13. Bar-Joseph, Z.; Gitter, A. et al. Studying and modelling dynamic biological processes using time-series gene expression data. Nat. Rev. Genet. 2012, 73(8), 552-64.
14. Ma, Y.; Dai, H.L. et al. Impact of warm ischemia on gene expression analysis in surgically removed biosamples.Anal. Biochem. 2012,423(2), 229-35.
15. Opel, K.L.; Chung, D. et al. A study of PCR inhibition mechanisms using real time PCR. J. Forens. Sci. 2010,55(1), 25-33.
16. Barber, R.D.; Harmer, D.W. et al. GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues. Physiol. Genomics 2005,2 7, 389-95. (Pubitemid 40898499)
17. Jacob, F.; Guertler, R. et al. Careful selection of reference genes is required for reliable performance of RT-qPCR in human normal and cancer cell lines. PLoS One 2013,8(3).
18. Rhinn, H.; Marchand-Leroux, C. et al. Housekeeping while brain's storming: validation of normalizing factors for gene expression studies in a murine model of traumatic brain injury. BMC Mol. Biol. Jul 8,2008,9,62.
19. Schmittgen, T.D.; Zakrajsek, B.A. Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR. J. Biochem. Biophys. Methods Nov 20,2000,46(1-2), 69-81.
20. Thellin, O.; Zorzi, W. et al. Housekeeping genes as internal standards: use and limits. J. Biotechnol. Oct 8,1999, 75(2-3), 291-5. (Pubitemid 29486627)
21. Yang, X.; Hatfield, J.T. et al. Bone to pick: the importance of evaluating reference genes for RT-qPCR quantification of gene expression in cra-niosynostosis and bone-related tissues and cells. BMC Res. Notes May 8, 2012,5,222; additional file 1.