Utility of a commercial PCR assay and a clinical prediction rule for detection of toxigenic clostridium difficile in asymptomatic carriers

Journal of Clinical Microbiology

Volumn 52, Issue 1, 2014, Pages 315-318

  Donskey, Curtis J ^a   Sunkesula, Venkata C K ^a   Jencson, Annette L ^a   Stone, Nimalie D ^b   Gould, Carolyn V ^b   McDonald, L Clifford ^b   Samore, Matthew ^c   Mayer, Jeanmarie ^c   Pacheco, Susan ^d   Sambol, Susan ^d   Petrella, Laurica ^d   Terry, Deborah ^d   Gerding, Dale N ^d  

^a LOUIS STOKES CLEVELAND VA MEDICAL CENTER   (United States)

^b CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

^c UNIVERSITY OF UTAH   (United States)

^d HINES VA HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC AGENT;

ABDOMEN; ADULT; AGED; ARTICLE; ASYMPTOMATIC INFECTION; BACTERIAL SHEDDING; BACTERIUM COLONY; BACTERIUM CONTAMINATION; BACTERIUM CULTURE; BACTERIUM DETECTION; CLOSTRIDIUM DIFFICILE; COHORT ANALYSIS; DIAGNOSTIC TEST ACCURACY STUDY; FEMALE; HUMAN; INGUINAL REGION; MALE; MIDDLE AGED; NONHUMAN; POLYMERASE CHAIN REACTION; POLYMERASE CHAIN REACTION SYSTEM; PREDICTIVE VALUE; PRIORITY JOURNAL; PROSPECTIVE STUDY; RECTUM; SENSITIVITY AND SPECIFICITY; THORAX; VERY ELDERLY;

ADULT; AGED; AGED, 80 AND OVER; BACTERIAL TOXINS; CARRIER STATE; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTIONS; DECISION SUPPORT TECHNIQUES; FEMALE; HUMANS; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; RECTUM;

EID: 84891512867     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01852-13     Document Type: Article

References (14)

1. Loo VG, Bourgault AM, Poirier L, Lamothe F, Michaud S, Turgeon N, Toye B, Beaudoin A, Frost EH, Gilca R, Brassard P, Dendukuri N, Béliveau C, Oughton M, Brukner I, Dascal A. 2011. Host and pathogen factors for Clostridium difficile infection and colonization. N. Engl. J. Med. 365:1693-1703. doi:http://dx.doi.org/10.1056/NEJMoa1012413.
2. Bender BS, Bennett R, Laughon BE, Greenough WB, III, Gaydos C, Sears SD, Forman MS, Bartlett JG. 1986. Is Clostridium difficile endemic in chronic-care facilities? Lancet ii:11-13.
3. Thomas DR, Bennett RG, Laughon BE, Greenough WB, III, Bartlett JG. 1990. Postantibiotic colonization with Clostridium difficile in nursing home patients. J. Am. Geriatr. Soc. 38:415-420.
4. Walker KJ, Gilliland SS, Vance-Bryan K, Moody JA, Larsson AJ, Rotschafer JC, Guay DR. 1993. Clostridium difficile colonization in residents of long-term care facilities: Prevalence and risk factors. J. Am. Geriatr. Soc. 41:940-946.
5. Simor AE, Yake SL, Tsimidis K. 1993. Infection due to Clostridium difficile among elderly residents of a long-term-care facility. Clin. Infect. Dis. 17:672-678. http://dx.doi.org/10.1093/clinids/17.4.672.
6. Riggs MM, Sethi AK, Zabarsky TF, Eckstein EC, Jump RL, Donskey CJ. 2007. Asymptomatic carriers are a potential source for transmission of epidemic and nonepidemic Clostridium difficile strains among long-term care facility residents. Clin. Infect. Dis. 45:992-998. http://dx.doi.org/10.1086/521854.
7. Clabots CR, Johnson S, Olson MM, Peterson LR, Gerding DN. 1992. Acquisition of Clostridium difficile by hospitalized patients: Evidence for colonized new admissions as a source of infection. J. Infect. Dis. 166:561-567. http://dx.doi.org/10.1093/infdis/166.3.561.
8. Guerrero DM, Becker JC, Eckstein EC, Kundrapu S, Deshpande A, Sethi AK, Donskey CJ. 2013. Asymptomatic carriage of toxigenic Clostridium difficile by hospitalized patients. J. Hosp. infect. 85:155-158. http://dx.doi.org/10.1016/j. jhin.2013.07.002.
9. Curry SR, Muto CA, Schlackman JL, Pasculle AW, Shutt KA, Marsh JW, Harrison LH. 2013. Use of multilocus variable number of tandem repeats analysis genotyping to determine the role of asymptomatic carriers in Clostridium difficile transmission. Clin. Infect. Dis. 57:1094-1102. http://dx.doi.org/10. 1093/cid/cit475.
10. Curry SR, Schlackman JL, Hamilton TM, Henderson TK, Brown NT, Marsh JW, Shutt KA, Brooks MM, Pasculle AW, Muto CA, Harrison LH. 2012. Perirectal swab surveillance for Clostridium difficile by use of selective broth preamplification and real-time PCR detection of tcdB. J. Clin. Microbiol. 50:4078-4082. http://dx.doi.org/10.1128/JCM.00679-11.
11. Gyorke CE, Wang S, Leslie JL, Cohen SH, Solnick JV, Polage CR. 2013. Evaluation of Clostridium difficile fecal load and limit of detection during a prospective comparison of two molecular tests, the illumigene C. difficile and Xpert C. difficile/Epi tests. J. Clin. Microbiol. 51:278-280. http://dx.doi.org/10.1128/JCM.02120-12.
12. Kundrapu S, Sunkesula VC, Jury LA, Sethi AK, Donskey CJ. 2012. Utility of perirectal swab specimens for diagnosis of Clostridium difficile infection. Clin. Infect. Dis. 55:1527-1530. http://dx.doi.org/10.1093/cid/cis707.
13. Cohen SH, Gerding DN, Johnson S, Kelly CP, Loo VG, McDonald LC, Pepin J, Wilcox MH. 2010. Clinical practice guidelines for Clostridium difficile infection in adults: 2010 update by the Society for Healthcare Epidemiology of America (SHEA) and the Infectious Diseases Society of America (IDSA). Infect. Control Hosp. Epidemiol. 31:431-455. http://dx.doi.org/10.1086/651706.
14. Nerandzic MM, Rackaityte E, Jury LA, Eckart K, Donskey CJ. 2013. Novel strategies for enhanced removal of persistent Bacillus anthracis surrogates and Clostridium difficile spores from skin. PLoS One 8:e68706. http://dx.doi.org/10. 1371/journal.pone.0068706.