Use of multilocus variable number of tandem repeats analysis genotyping to determine the role of asymptomatic carriers in Clostridium difficile transmission

Clinical Infectious Diseases

Volumn 57, Issue 8, 2013, Pages 1094-1102

  Curry, Scott R ^a   Muto, Carlene A ^a,b   Schlackman, Jessica L ^a   Pasculle, A William ^a   Shutt, Kathleen A ^a   Marsh, Jane W ^a   Harrison, Lee H ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF PITTSBURGH MEDICAL CENTER   (United States)

Author keywords

Clostridium difficile; genotyping; MLVA; screening; transmission

Indexed keywords

CLOSTRIDIUM DIFFICILE TOXIN A; CLOSTRIDIUM DIFFICILE TOXIN B;

ARTICLE; ASYMPTOMATIC INFECTION; BACTERIAL TRANSMISSION; BACTERIUM CULTURE; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM DIFFICILE INFECTION; DISEASE CARRIER; DISEASE CLASSIFICATION; DISEASE SURVEILLANCE; EPIDEMIC; FECES ANALYSIS; GENETIC PROCEDURES; GENOTYPE; HOSPITAL READMISSION; HUMAN; INCUBATION TIME; INFECTION CONTROL; LABORATORY DIAGNOSIS; MAJOR CLINICAL STUDY; MOLECULAR EPIDEMIOLOGY; MOLECULAR PHYLOGENY; MOLECULAR TYPING; MULTILOCUS VARIABLE NUMBER OF TANDEM REPEAT ANALYSIS; NONHUMAN; PRIORITY JOURNAL; SCREENING TEST; VANCOMYCIN RESISTANT ENTEROCOCCUS; VARIABLE NUMBER OF TANDEM REPEAT;

CLOSTRIDIUM DIFFICILE; GENOTYPING; MLVA; SCREENING; TRANSMISSION;

CARRIER STATE; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTIONS; CROSS INFECTION; DNA, BACTERIAL; GENOTYPE; HUMANS; MASS SCREENING; MINISATELLITE REPEATS; MOLECULAR EPIDEMIOLOGY; PENNSYLVANIA; PUBLIC HEALTH SURVEILLANCE;

EID: 84884510057     PISSN: 10584838     EISSN: 15376591     Source Type: Journal    
DOI: 10.1093/cid/cit475     Document Type: Article

References (23)

1. Clabots CR, Johnson S, Olson MM, Peterson LR, Gerding DN. Acquisition of Clostridium difficile by hospitalized patients: evidence for colonized new admissions as a source of infection. J Infect Dis 1992; 166:561-7.
2. Johnson S, Clabots CR, Linn FV, Olson MM, Peterson LR, Gerding DN. Nosocomial Clostridium difficile colonisation and disease. Lancet 1990; 336:97-100. (Pubitemid 20222816)
3. McFarland LV, Mulligan ME, Kwok RY, Stamm WE. Nosocomial acquisition of Clostridium difficile infection. N Engl J Med 1989; 320: 204-10. (Pubitemid 19034145)
4. Samore MH, DeGirolami PC, Tlucko A, Lichtenberg DA, Melvin ZA, Karchmer AW. Clostridium difficile colonization and diarrhea at a tertiary care hospital. Clin Infect Dis 1994; 18:181-7. (Pubitemid 24055256)
5. Shim JK, Johnson S, Samore MH, Bliss DZ, Gerding DN. Primary symptomless colonisation by Clostridium difficile and decreased risk of subsequent diarrhoea. Lancet 1998; 351:633-6. (Pubitemid 28098446)
6. Siddiqui AH, Harris AD, Hebden J, Wilson PD, Morris JG Jr, Roghmann MC. The effect of active surveillance for vancomycin-resistant enterococci in high-risk units on vancomycin-resistant enterococci incidence hospital-wide. Am J Infect Control 2002; 30:40-3. (Pubitemid 34155281)
7. Robicsek A, Beaumont JL, Paule SM, et al. Universal surveillance for methicillin-resistant Staphylococcus aureus in 3 affiliated hospitals. Ann Intern Med 2008; 148:409-18. (Pubitemid 351665440)
8. Curry SR, Schlackman JL, Hamilton TM, et al. Perirectal swab surveillance for Clostridium difficile by use of selective broth preamplification and real-time PCR detection of tcdB. J Clin Microbiol 2011; 49:3788-93.
9. Mekonen ET, Gerding DN, Sambol SP, et al. Predominance of a single restriction endonuclease analysis group with intrahospital subgroup diversity among Clostridium difficile isolates at two Chicago hospitals. Infect Control Hosp Epidemiol 2002; 23:648-52. (Pubitemid 35315986)
10. Marsh JW, O'Leary MM, Shutt KA, et al. Multilocus variable-number tandem-repeat analysis for investigation of Clostridium difficile transmission in hospitals. J Clin Microbiol 2006; 44:2558-66. (Pubitemid 44092427)
11. Muto CA, Blank MK, Marsh JW, et al. Control of an outbreak of infection with the hypervirulent Clostridium difficile BI strain in a university hospital using a comprehensive "bundle" approach. Clin Infect Dis 2007; 45:1266-73. (Pubitemid 351411898)
12. Marsh JW, O'Leary MM, Shutt KA, et al. Multilocus variable-number tandem-repeat analysis and multilocus sequence typing reveal genetic relationships among Clostridium difficile isolates genotyped by restriction endonuclease analysis. J Clin Microbiol 2010; 48:412-8.
13. Curry SR, Marsh JW, Muto CA, O'Leary MM, Pasculle AW, Harrison LH. tcdC genotypes associated with severe TcdC truncation in an epidemic clone and other strains of Clostridium difficile. J Clin Microbiol 2007; 45:215-21. (Pubitemid 46106429)
14. Dingle KE, Griffiths D, Didelot X, et al. Clinical Clostridium difficile: clonality and pathogenicity locus diversity. PLoS One 2011; 6:e19993.
15. Spigaglia P, Mastrantonio P. Molecular analysis of the pathogenicity locus and polymorphism in the putative negative regulator of toxin production (TcdC) among Clostridium difficile clinical isolates. J Clin Microbiol 2002; 40:3470-5. (Pubitemid 34977470)
16. McDonald LC, Coignard B, Dubberke E, et al. Recommendations for surveillance of Clostridium difficile-associated disease. Infect Control Hosp Epidemiol 2007; 28:140-5. (Pubitemid 46374153)
17. Riggs MM, Sethi AK, Zabarsky TF, Eckstein EC, Jump RL, Donskey CJ. Asymptomatic carriers are a potential source for transmission of epidemic and nonepidemic Clostridium difficile strains among long-term care facility residents. Clin Infect Dis 2007; 45:992-8. (Pubitemid 47580347)
18. Shaughnessy MK, Micielli RL, DePestel DD, et al. Evaluation of hospital room assignment and acquisition of Clostridium difficile infection. Infect Control Hosp Epidemiol 2011; 32:201-6.
19. Eckstein BC, Adams DA, Eckstein EC, et al. Reduction of Clostridium difficile and vancomycin-resistant Enterococcus contamination of environmental surfaces after an intervention to improve cleaning methods. BMC Infect Dis 2007; 7:61.
20. Loo VG, Bourgault AM, Poirier L, et al. Host and pathogen factors for Clostridium difficile infection and colonization. N Engl J Med 2011; 365:1693-703.
21. Walker AS, Eyre DW, Wyllie DH, et al. Characterisation of Clostridium difficile hospital ward-based transmission using extensive epidemiological data and molecular typing. PLoS Med 2012; 9: e1001172.
22. Lawley TD, Clare S, Deakin LJ, et al. Use of purified Clostridium difficile spores to facilitate evaluation of health care disinfection regimens. Appl Environ Microbiol 2010; 76:6895-900.
23. Perez J, Springthorpe VS, Sattar SA. Clospore: a liquid medium for producing high titers of semi-purified spores of Clostridium difficile. J AOAC Int 2011; 94:618-26.