Residues Arg703, Asp777, and Arg781 of the rnase H domain of hepatitis B virus polymerase are critical for viral DNA synthesis

Journal of Virology

Volumn 88, Issue 1, 2014, Pages 154-163

  Ko, Chunkyu ^a   Shin, Youn Chul ^a   Park, Woo Jin ^a   Kim, Seungtaek ^c   Kim, Jonghwa ^b   Ryua, Wang Shick ^a  

^a Yonsei University   (South Korea)

^b Samsung Medical Center   (South Korea)

^c Yonsei University College of Medicine   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; ARGININE; MUTANT PROTEIN; RIBONUCLEASE H; VIRUS DNA; VIRUS RNA;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CONTROLLED STUDY; DNA SYNTHESIS; ENZYME ACTIVITY; ENZYME DEFECT; HEPATITIS B VIRUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; REVERSE TRANSCRIPTION; RNA TRANSLATION; RNA TRANSPORT; SOUTHERN BLOTTING; VIRUS CAPSID; VIRUS TRANSCRIPTION;

ARGININE; ASPARTIC ACID; DNA, VIRAL; HEP G2 CELLS; HEPATITIS B VIRUS; HUMANS; MUTATION; RIBONUCLEASE H; RNA-DIRECTED DNA POLYMERASE;

EID: 84890880422     PISSN: 0022538X     EISSN: 10985514     Source Type: Journal    
DOI: 10.1128/JVI.01916-13     Document Type: Article

References (31)

1. Seeger C, Zoulim F, Mason W. 2007. Hepadnaviruses, p 2977-3029. In Knipe DM, Howley PM, Griffin DE, Lamb RA, Martin MA, Roizman B, Straus SE (ed), Fields virology, 5th ed, vol 2. Lippincott Williams & Wilkins, Philadelphia, PA.
2. Hirsch RC, Loeb DD, Pollack JR, Ganem D. 1991. Cis-acting sequences required for encapsidation of duck hepatitis B virus pregenomic RNA. J. Virol. 65:3309-3316.
3. Jeong JK, Yoon GS, Ryu WS. 2000. Evidence that the 5=-end cap structure is essential for encapsidation of hepatitis B virus pregenomic RNA. J. Virol. 74:5502-5508. http://dx.doi.org/10.1128/JVI.74.12.5502-5508.2000.
4. Junker-Niepmann M, Bartenschlager R, Schaller H. 1990. A short cisacting sequence is required for hepatitis B virus pregenome encapsidation and sufficient for packaging of foreign RNA. EMBO J. 9:3389-3396.
5. Tavis JE, Perri S, Ganem D. 1994. Hepadnavirus reverse transcription initiates within the stem-loop of the RNA packaging signal and employs a novel strand transfer. J. Virol. 68:3536-3543.
6. Wang GH, Seeger C. 1993. Novel mechanism for reverse transcription in hepatitis B viruses. J. Virol. 67:6507-6512.
7. Loeb DD, Hirsch RC, Ganem D. 1991. Sequence-independent RNA cleavages generate the primers for plus strandDNAsynthesis in hepatitis B viruses: implications for other reverse transcribing elements.EMBOJ. 10:3533-3540.
8. Staprans S, Loeb DD, Ganem D. 1991. Mutations affecting hepadnavirus plus-strand DNA synthesis dissociate primer cleavage from translocation and reveal the origin of linear viral DNA. J. Virol. 65:1255-1262.
9. Nassal M. 2008. Hepatitis B viruses: reverse transcription a different way. Virus Res. 134:235-249. http://dx.doi.org/10.1016/j.virusres.2007.12.024.
10. Chen Y, Robinson WS, Marion PL. 1992. Naturally occurring point mutation in theCterminus of the polymerase gene prevents duck hepatitis B virus RNA packaging. J. Virol. 66:1282-1287.
11. Chen Y, Robinson WS, Marion PL. 1994. Selected mutations of the duck hepatitis B virus P gene RNase H domain affect both RNA packaging and priming of minus-strand DNA synthesis. J. Virol. 68:5232- 5238.
12. Chen Y, Marion PL. 1996. Amino acids essential for RNase H activity of hepadnaviruses are also required for efficient elongation of minus-strand viral DNA. J. Virol. 70:6151-6156.
13. Seeger C, Leber EH, Wiens LK, Hu J. 1996. Mutagenesis of a hepatitis B virus reverse transcriptase yields temperature-sensitive virus. Virology 222:430-439. http://dx.doi.org/10.1006/viro.1996.0440.
14. Alber T. 1989. Mutational effects on protein stability. Annu. Rev. Biochem. 58:765-798. http://dx.doi.org/10.1146/annurev.bi.58.070189.004001.
15. Shin Y-C, Park S, Ryu W-S. 2011. A conserved arginine residue in the terminal protein domain of hepatitis B virus polymerase is critical for RNA pregenome encapsidation. J. Gen. Virol. 92:1809-1816. http://dx .doi.org/10.1099/vir.0.031914-0.
16. Kim S, Wang H, Ryu WS. 2010. Incorporation of eukaryotic translation initiation factor eIF4E into viral nucleocapsids via interaction with hepatitis B virus polymerase. J. Virol. 84:52-58. http://dx.doi.org/10.1128/JVI.01232-09.
17. Galibert F, Mandart E, Fitoussi F, Tiollais P, Charnay P. 1979. Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli. Nature 281:646-650. http://dx.doi.org/10.1038/281646a0.
18. Lee J, Lee H-J, Shin M-K, Ryu W-S. 2004. Versatile PCR-mediated insertion or deletion mutagenesis. Biotechniques 36:398-400.
19. Tang H, McLachlan A. 2001. Transcriptional regulation of hepatitis B virus by nuclear hormone receptors is a critical determinant of viral tropism. Proc. Natl. Acad. Sci. U. S. A. 98:1841-1846. http://dx.doi.org/10 .1073/pnas.98.4.1841.
20. Shin M-K, Lee J, Ryu W-S. 2004. A novel cis-acting element facilitates minus-strand DNA synthesis during reverse transcription of the hepatitis B viruses genome. J. Virol. 78:6252-6262. http://dx.doi.org/10.1128/JVI .78.12.6252-6262.2004.
21. Lewellyn EB, Loeb DD. 2011. The arginine clusters of the carboxyterminal domain of the core protein of hepatitis B virus make pleiotropic contributions to genome replication. J. Virol. 85:1298-1309. http://dx.doi .org/10.1128/JVI.01957-10.
22. Cha MY, Ryu DK, Jung HS, Chang HE, Ryu WS. 2009. Stimulation of hepatitis B virus genome replication by HBx is linked to both nuclear and cytoplasmic HBx expression. J. Gen. Virol. 90:978-986. http://dx.doi.org /10.1099/vir.0.009928-0.
23. Chomczynski P, Sacchi N. 2006. The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on. Nat. Protoc. 1:581-585. http://dx.doi.org/10 .1038/nprot.2006.83.
24. Kim S, Lee J, Ryu WS. 2009. Four conserved cysteine residues of the hepatitis B virus polymerase are critical for RNA pregenome encapsidation. J. Virol. 83:8032-8040. http://dx.doi.org/10.1128/JVI.00332-09.
25. Abraham TM, Lewellyn EB, Haines KM, Loeb DD. 2008. Characteriza- tion of the contribution of spliced RNAs of hepatitis B virus to DNA synthesis in transfected cultures of Huh7 and HepG2 cells. Virology 379: 30-37. http://dx.doi.org/10.1016/j.virol.2008.06.021.
26. Haines KM, Loeb DD. 2007. The sequence of the RNA primer and the DNA template influence the initiation of plus-strand DNA synthesis in hepatitis B virus. J. Mol. Biol. 370:471-480. http://dx.doi.org/10.1016/j .jmb.2007.04.057.
27. Tavis JE, Cheng X, Hu Y, Totten M, Cao F, Michailidis E, Aurora R, Meyers MJ, Jacobsen EJ, Parniak MA, Sarafianos SG. 2013. The hepatitis B virus ribonuclease H is sensitive to inhibitors of the human immunodeficiency virus ribonuclease H and integrase enzymes. PLoS Pathog. 9:e1003125. http://dx.doi.org/10.1371/journal.ppat.1003125.
28. Bartenschlager R, Junker-Niepmann M, Schaller H. 1990. The P gene product of hepatitis B virus is required as a structural component for genomic RNA encapsidation. J. Virol. 64:5324-5332.
29. Kwon H, Lok AS. 2011. Hepatitis B therapy. Nat. Rev. Gastroenterol. Hepatol. 8:275-284. http://dx.doi.org/10.1038/nrgastro.2011.33.
30. Zoulim F. 2012. Are novel combination therapies needed for chronic hepatitis B? Antiviral Res. 96:256-259. http://dx.doi.org/10.1016/j.antiviral.2012 .09.006.
31. Hu Y, Cheng X, Cao F, Huang A, Tavis JE. 2013. β-Thujaplicinol inhibits hepatitis B virus replication by blocking the viral ribonuclease H activity. Antiviral Res. 99:221-229. http://dx.doi.org/10.1016/j.antiviral .2013.06.007.