Pre-pore oligomer formation by Vibrio cholerae cytolysin: Insights from a truncated variant lacking the pore-forming pre-stem loop

Biochemical and Biophysical Research Communications

Volumn 443, Issue 1, 2014, Pages 189-193

  Paul, Karan ^a   Chattopadhyay, Kausik ^a  

^a INDIAN INSTITUTE OF SCIENCE EDUCATION AND RESEARCH MOHALI   (India)

Author keywords

Bacterial protein toxin; Pore forming toxin; Vibrio cholerae cytolysin

Indexed keywords

CYTOLYSIN; MEMBRANE PROTEIN; OLIGOMER;

ARTICLE; HEMOLYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; TARGET CELL; VIBRIO CHOLERAE;

BACTERIA (MICROORGANISMS); EUKARYOTA; VIBRIO CHOLERAE;

BACTERIAL PROTEIN TOXIN; CD; CIRCULAR DICHROISM; FLUORESCENCE RESONANCE ENERGY TRANSFER; FRET; PFT; PORE-FORMING TOXIN; VCC; VIBRIO CHOLERAE CYTOLYSIN;

BACTERIAL PROTEINS; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; HUMANS; MODELS, MOLECULAR; PERFORIN; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; VIBRIO CHOLERAE;

EID: 84890857076     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2013.11.078     Document Type: Article

References (20)

1. Alouf J.E., Popoff M.R. The Comprehensive Sourcebook of Bacterial Protein Toxins 2006, Academic Press.
2. Iacovache I., Bischofberger M., van der Goot F.G. Structure and assembly of pore-forming proteins. Curr. Opin. Struct. Biol. 2010, 20:241-246.
3. Iacovache I., van der Goot F.G., Pernot L. Pore formation: an ancient yet complex form of attack. Biochim. Biophys. Acta (BBA) - Biomembr. 2008, 1778:1611-1623.
4. Gonzalez M., Bischofberger M., Pernot L., van der Goot F., Frêche B. Bacterial pore-forming toxins: the (w)hole story?. Cell. Mol. Life Sci. 2008, 65:493-507.
5. Tilley S.J., Saibil H.R. The mechanism of pore formation by bacterial toxins. Curr. Opin. Struct. Biol. 2006, 16:230-236.
6. Heuck A.P., Tweten R.K., Johnson A.E. Beta-barrel pore-forming toxins: intriguing dimorphic proteins. Biochemistry 2001, 40:9065-9073.
7. Walker B., Braha O., Cheley S., Bayley H. An intermediate in the assembly of a pore-forming protein trapped with a genetically-engineered switch. Chem. Biol. 1995, 2:99-105.
8. Walker B., Krishnasastry M., Zorn L., Bayley H. Assembly of the oligomeric membrane pore formed by Staphylococcal alpha-hemolysin examined by truncation mutagenesis. J. Biol. Chem. 1992, 267:21782-21786.
9. Bayley H., Jayasinghe L., Wallace M. Prepore for a breakthrough. Nat. Struct. Mol. Biol. 2005, 12:385-386.
10. Valeva A., Palmer M., Bhakdi S. Staphylococcal alpha-toxin: formation of the heptameric pore is partially cooperative and proceeds through multiple intermediate stages. Biochemistry 1997, 36:13298-13304.
11. Song L., Hobaugh M.R., Shustak C., Cheley S., Bayley H., Gouaux J.E. Structure of staphylococcal alpha-hemolysin, a heptameric transmembrane pore. Science 1996, 274:1859-1866.
12. De S., Olson R. Crystal structure of the Vibrio cholerae cytolysin heptamer reveals common features among disparate pore-forming toxins. Proc. Natl. Acad. Sci. USA 2011, 108:7385-7390.
13. Olson R., Gouaux E. Crystal structure of the Vibrio cholerae cytolysin (VCC) pro-toxin and its assembly into a heptameric transmembrane pore. J. Mol. Biol. 2005, 350:997-1016.
14. Honda T., Finkelstein R.A. Purification and characterization of a hemolysin produced by Vibrio cholerae biotype El Tor: another toxic substance produced by cholera vibrios. Infect. Immun. 1979, 26:1020-1027.
15. Ichinose Y., Yamamoto K., Nakasone N., Tanabe M.J., Takeda T., Miwatani T., Iwanaga M. Enterotoxicity of El Tor-like hemolysin of non-O1 Vibrio cholerae. Infect. Immun. 1987, 55:1090-1093.
16. Zitzer A., Walev I., Palmer M., Bhakdi S. Characterization of Vibrio cholerae El Tor cytolysin as an oligomerizing pore-forming toxin. Med. Microbiol. Immunol. 1995, 184:37-44.
17. Lohner S., Walev I., Boukhallouk F., Palmer M., Bhakdi S., Valeva A. Pore formation by Vibrio cholerae cytolysin follows the same archetypical mode as {beta}-barrel toxins from gram-positive organisms. FASEB J. 2009, 23:2521-2528.
18. Paul K., Chattopadhyay K. Unfolding distinguishes the Vibrio cholerae cytolysin precursor from the mature form of the toxin. Biochemistry 2011, 50:3936-3945.
19. Paul K., Chattopadhyay K. Single point mutation in Vibrio cholerae cytolysin compromises membrane pore-formation mechanism of the toxin. FEBS J. 2012, 279:4039-4051.
20. Rai A.K., Paul K., Chattopadhyay K. Functional mapping of the lectin activity site on the beta-prism domain of Vibrio cholerae cytolysin: implications for the membrane pore-formation mechanism of the toxin. J. Biol. Chem. 2013, 288:1665-1673.