A Pasteurella multocida sialyltransferase displaying dual trans-sialidase activities for production of 3'-sialyl and 6'-sialyl glycans

Journal of Biotechnology

Volumn 170, Issue 1, 2014, Pages 60-67

  Guo, Yao ^a   Jers, Carsten ^a   Meyer, Anne S ^a   Arnous, Anis ^a   Li, Haiying ^b   Kirpekar, Finn ^b   Mikkelsen, Jørn D ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

Author keywords

Casein glycomacropeptide as source for sialic acid; Enzyme expression and purification; Human milk oligosaccharides; Kinetics of trans sialidase; Response surface

Indexed keywords

CASEIN GLYCOMACROPEPTIDE; GALACTO-OLIGOSACCHARIDES; HUMAN MILK OLIGOSACCHARIDES; PASTEURELLA MULTOCIDA; RESPONSE SURFACE; RESPONSE SURFACE DESIGNS; SIALIC ACIDS; TRANS-SIALIDASES;

CARBOXYLIC ACIDS; ENZYMES; SACCHARIFICATION; SUGARS; SURFACE PROPERTIES; SYNTHESIS (CHEMICAL);

CASEIN;

3' SIALYLLACTOSE; 6' SIALYLLACTOSE; ALPHA 2,6 SIALIDASE; GLYCAN; GLYCOPROTEIN; LACTOSE; SIALIC ACID; SIALYLTRANSFERASE; UNCLASSIFIED DRUG;

ARTICLE; ENZYME ACTIVITY; MASS FRAGMENTOGRAPHY; NONHUMAN; PASTEURELLA MULTOCIDA; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION; TEMPERATURE; THIN LAYER CHROMATOGRAPHY;

2-O-(P-NITROPHENYL)-Α-D-N-ACETYLNEURAMINIC ACID; 4-METHYLUMBELLIFERYL Β-D-LACTOSIDE; ADENOSINE-5′-TRIPHOSPHATE; ATP; CAPILLARY LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY; CASEIN GLYCOMACROPEPTIDE; CASEIN GLYCOMACROPEPTIDE AS SOURCE FOR SIALIC ACID; CGMP; CMP-NEUAC; COLUMN VOLUME; CTP; CV; CYTIDINE-5′-MONOPHOSPHO-N-ACETYLNEURAMINIC ACID; CYTIDINE-5′-TRIPHOSPHATE; ENZYME EXPRESSION AND PURIFICATION; GALACTOOLIGOSACCHARIDES; GALNAC; GOS; HIGH-PERFORMANCE ANION EXCHANGE CHROMATOGRAPHY; HMOS; HPAEC-PAD; HUMAN MILK OLIGOSACCHARIDES; KINETICS OF TRANS-SIALIDASE; LACMU; LC-MS; MALDI-MS; MATRIX-ASSISTED LASER DESORPTION/IONIZATION MASS SPECTROMETRY; N-ACETYLGALACTOSAMINE; N-ACETYLNEURAMINIC ACID; N-HIS6-TAG; N-TERMINAL HIS(6)-TAG; NDV; NEU5AC; NEWCASTLE DISEASE VIRUS; PASTEURELLA MULTOCIDA SIALYLTRANSFERASE; PEP; PGC; PHOSPHOENOLPYRUVATE; PMST; PNP-NEU5AC; POROUS GRAPHITIC CARBON; RESPONSE SURFACE; RESPONSE SURFACE METHODOLOGY; RSM; SDS-PAGE; SIA-GOS; SIALYLATED GALACTOOLIGOSACCHARIDES; SODIUM DODECYL SULFATE POLYACRYLAMIDE GEL ELECTROPHORESIS; THIN-LAYER CHROMATOGRAPHY; TLC;

BACTERIAL PROTEINS; GLYCOPROTEINS; HUMANS; HYDROGEN-ION CONCENTRATION; LACTOSE; MILK PROTEINS; N-ACETYLNEURAMINIC ACID; NEURAMINIDASE; OLIGOSACCHARIDES; PASTEURELLA MULTOCIDA; RECOMBINANT PROTEINS; SIALYLTRANSFERASES; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TEMPERATURE;

EID: 84890846001     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2013.11.013     Document Type: Article

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