Identification of inhibitors of triacylglyceride accumulation in muscle cells: Comparing HTS results from 1536-well plate-based and high-content platforms

Journal of Biomolecular Screening

Volumn 19, Issue 1, 2014, Pages 77-87

  Sugarman, Eliot ^a   Koo, Ada ^a   Suyama, Eigo ^a   Ruidiaz, Manuel E ^b   Heynen Genel, Susanne ^b   Nguyen, Kevin H ^a   Vasile, Stefan ^a   Soundarapandian, Mangala M ^a   Vega, Rick B ^a   Kelly, Daniel P ^a   Smith, Layton H ^a   Malany, Siobhan ^a  

^a BURNHAM INSTITUTE   (United States)

^b Burham Institute   (United States)

Author keywords

1536 well high throughput screening; H9c2 cardiomyocytes; high content analysis; human primary myocytes; lipid accumulation; Nile red fluorescence; phenotypic screening

Indexed keywords

4',6 DIAMIDINO 2 PHENYLINDOLE; FLUORESCENT DYE; OLEIC ACID; PHOSPHOLIPID; TRIACYLGLYCERIDE; TRIACYLGLYCEROL; UNCLASSIFIED DRUG;

ARTICLE; ASSAY; CELL COUNT; CELL CULTURE; CELL NUCLEUS; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOPLASM; CYTOTOXICITY; FILTER; HUMAN; HUMAN CELL; INCUBATION TIME; LIPID METABOLISM; LIPID STORAGE; LIPOTOXICITY; MICROPLATE READER; MUSCLE CELL; MYOBLAST; NATIONAL HEALTH ORGANIZATION; PATHOGENESIS; PRIORITY JOURNAL;

1536-WELL HIGH-THROUGHPUT SCREENING; H9C2 CARDIOMYOCYTES; HIGH-CONTENT ANALYSIS; HUMAN PRIMARY MYOCYTES; LIPID ACCUMULATION; NILE RED FLUORESCENCE; PHENOTYPIC SCREENING;

CELL CULTURE TECHNIQUES; CELL LINE; DRUG DISCOVERY; DRUG EVALUATION, PRECLINICAL; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; LIPID METABOLISM; MUSCLE FIBERS, SKELETAL; REPRODUCIBILITY OF RESULTS; TRIGLYCERIDES;

EID: 84890845941     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057113501198     Document Type: Article

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