High-content imaging technology for the evaluation of drug-induced steatosis using a multiparametric cell-based assay

Journal of Biomolecular Screening

Volumn 17, Issue 3, 2012, Pages 394-400

  Donato, M Teresa ^a,b,c   Tolosa, Laia ^a   Jiménez, Nuria ^a   Castell, José V ^a,b,c   Gómez Lechón, M José ^a,b  

^a HOSPITAL UNIVERSITARIO LA FE   (Spain)

^b INSTITUTO DE SALUD CARLOS III   (Spain)

^c UNIVERSITY OF VALENCIA   (Spain)

Author keywords

BODIPY493 503; cultured cells; drug induced steatosis; hepatotoxicity; high content screening

Indexed keywords

ACETYLSALICYLIC ACID; AMIKACIN; AMIODARONE; CITRIC ACID; CLOFIBRATE; COLCHICINE; CUMENE HYDROPEROXIDE; CYCLOSPORIN A; DIDANOSINE; DOXYCYCLINE; ETHIONINE; FENOFIBRATE; FIALURIDINE; LIPID; ROTENONE; STAVUDINE; TAMOXIFEN; TERT BUTYL HYDROPEROXIDE; TETRACYCLINE; TETRAMETHYLRHODAMINE ETHYL ESTER; TIANEPTINE; TICLOPIDINE; VALPROIC ACID; ZIDOVUDINE;

ARTICLE; CELL CULTURE; CELL STRAIN HEPG2; CELL VIABILITY; CONTROLLED STUDY; DIAGNOSTIC PROCEDURES; DRUG IDENTIFICATION; DRUG INDUCED DISEASE; HIGH CONTENT IMAGING; HUMAN; HUMAN CELL; LIPID STORAGE; LIVER INJURY; LIVER TOXICITY; MITOCHONDRIAL MEMBRANE POTENTIAL; PRIORITY JOURNAL; STEATOSIS;

BIOLOGICAL MARKERS; CELL LINE; CELL SURVIVAL; DRUG EVALUATION, PRECLINICAL; FATTY LIVER; FLUORESCENT DYES; HEP G2 CELLS; HUMANS; LIVER; MICROSCOPY, FLUORESCENCE; REACTIVE OXYGEN SPECIES;

EID: 84859057446     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057111427586     Document Type: Article

References (15)

1. Fromenty, B.; Pessayre, D. Impaired Mitochondrial Function in Microvesicular Steatosis: Effects of Drugs, Ethanol, Hormones and Cytokines. J. Hepatol, 1997, 26, 43-53.
2. Begriche, K.; Massart, J.; Robin, M. A.; Borgne-Sanchez, A.; Fromenty, B. Drug-Induced Toxicity onMitochondria and Lipid Metabolism: Mechanistic Diversity and Deleterious Consequences for the Liver. J. Hepatol. 2011, 54, 773-794.
3. Amacher, D. E. Strategies for the Early Detection of Drug- Induced Hepatic Steatosis in Preclinical Drug Safety Evaluation Studies. Toxicology 2011, 279, 10-18.
4. McMillian, M. K.; Grant, E. R.; Zhong, Z.; Parker, J. B.; Li, L.; Zivin, R. A.; Burczynski, M. E.; Johnson, M. D. Nile Red Binding toHepG2 Cells: An Improved Assay for In Vitro Studies of Hepatosteatosis. In Vitr. Mol. Toxicol. 2001, 14, 177-190. (Pubitemid 34326670)
5. Fujimura, H.; Murakami, N.; Kurabe, M.; Toriumi, W. In Vitro Assay for Drug-Induced Hepatosteatosis Using Rat Primary Hepatocytes, a Fluorescent Lipid Analog and Gene Expression Analysis. J. Appl. Toxicol. 2009, 29, 356-363.
6. Donato, M. T.; Martínez-Romero, A.; Jiménez, N.; Negro, A.; Herrera, G.; Castell, J. V.; O'Connor, J. E.; Gómez-Lechón, M. J. Cytometric Analysis for Drug-Induced Steatosis in HepG2 Cells. Chem. Biol. Interact. 2009, 181, 417-423.
7. O'Brien, P. J.; Irwin, W.; Diaz, D.; Howard-Cofield, E.; Krejsa, C. M.; Slaughter, M. R.; Gao, B.; Kaludercic, N.; Angeline, A.; Bernardi, P.; Brain, P.; Hougham, C. High Concordance of Drug-Induced Human Hepatotoxicity with In Vitro Cytotoxicity Measured in a Novel Cell-Based Model Using High Content Screening. Arch. Toxicol. 2006, 80, 580-604. (Pubitemid 44214526)
8. Xu, J. J.; Henstock, P. V.; Dunn, M. C.; Smith, A. R.; Chabot, J. R.; de Graaf, D. Cellular Imaging Predictions of Clinical Drug- Induced Liver Injury. Toxicol. Sci. 2008, 105, 97-105.
9. Letteron, P.; Fromenty, B.; Terris, B.; Degott, C.; Pessayre, D. Acute and Chronic Hepatic Steatosis Lead to In Vivo Lipid Peroxidation in Mice. J. Hepatol. 1996, 24, 200-208. (Pubitemid 26059117)
10. Gómez-Lechón, M. J.; Tolosa, L.; Castell, J. V.; Donato, M. T. Mechanism-Based Selection of Compounds for the Development of Innovative In Vitro Approaches to Hepatotoxicity Studies in the LIINTOP Project. Toxicol. In Vitro 2010, 24, 1879-1889.
11. Tilmant, K.; Gerets, H. H.; Dhalluin, S.; Hanon, E.; Depelchin, O.; Cossu-Leguille, C.; Vasseur, P.; Atienzar, F. A. Comparison of aGenomic and a Multiplex Cell Imaging Approach for the Detection of Phospholipidosis. Toxicol. In Vitro 2011;25(7): 1414-1424.
12. Rezzani, R. Exploring Cyclosporine A-Side Effects and the Protective Role-Played by Antioxidants: The Morphological and Immunohistochemical Studies. Histol. Histopathol. 2006, 21, 301-316. (Pubitemid 43033739)
13. Tong, V.; Teng, X. W.; Chang, T. K.; Abbott, F. S. Valproic Acid II: Effects on Oxidative Stress, Mitochondrial Membrane Potential, and Cytotoxicity in Glutathione-Depleted Rat Hepatocytes. Toxicol. Sci. 2005, 86, 436-443. (Pubitemid 41418656)
14. Kleiner, D. E.; Gaffey, M. J.; Sallie, R.; Tsokos, M.; Nichols, L.; McKenzie, R.; Straus, S. E.; Hoofnagle, J. H. Histopathologic Changes Associated with Fialuridine Hepatotoxicity. Mod. Pathol. 1997, 10, 192-199. (Pubitemid 27130467)
15. Meneses-Lorente, G.; Watt, A.; Salim, K.; Gaskell, S. J.; Muniappa, N.; Lawrence, J.; Guest, P. C. Identification of Early Proteomic Markers for Hepatic Steatosis. Chem. Res. Toxicol. 2006, 19, 986-998. (Pubitemid 44384829)