Automated analysis of intracellular calcium fluorescence in rat organotypic hippocampal cultures: Comparison to a manual, observer based method

Journal of Neuroscience Methods

Volumn 223, Issue , 2014, Pages 20-29

  Watters, Orla ^a,b   Pickering, Mark ^a   Murphy, Robert ^a   Murphy, Keith J ^a   O'Connor, John J ^a  

^a UNIVERSITY COLLEGE DUBLIN   (Ireland)

^b ROYAL COLLEGE OF SURGEONS IN IRELAND   (Ireland)

Author keywords

Automated analysis; Calcium imaging; Glutamate; Hippocampus; Organotypic cultures; Tumor necrosis factor alpha

Indexed keywords

CALCIUM;

ACCURACY; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; AUTOMATION; BRAIN SLICE; CALCIUM CELL LEVEL; CONTROLLED STUDY; FEMALE; FLUORESCENCE MICROSCOPY; HIPPOCAMPAL NEURONAL CULTURE; IMAGE ANALYSIS; INTERMETHOD COMPARISON; MALE; NONHUMAN; OBSERVATIONAL METHOD; OBSERVER VARIATION; ORGANOTYPIC HIPPOCAMPAL CULTURE; PRIORITY JOURNAL; RAT; SLICE CULTURE; TIME SERIES ANALYSIS;

AUTOMATED ANALYSIS; BALANCED SALT SOLUTION; BSS; BUFFERED SALT SOLUTION; CALCIUM IMAGING; COMPUTERISED TOMOGRAPHY; CT; EARLES BALANCED SALT SOLUTION; EBSS; GLUTAMATE; GRAPHICAL USER INTERFACE; GUI; HIPPOCAMPUS; MEM; MINIMUM ESSENTIAL MEDIUM; ORGANOTYPIC CULTURES; PET; POSITRON EMISSION TOMOGRAPHY; REGIONS OF INTEREST; ROIS; SEM; STANDARD ERROR OF THE MEAN; TNF-Α; TUMOR NECROSIS FACTOR ALPHA; TUMOR NECROSIS FACTOR-Α;

ALGORITHMS; ANIMALS; ANIMALS, NEWBORN; AUTOMATIC DATA PROCESSING; CALCIUM; FEMALE; FLUORESCENT DYES; GLUTAMIC ACID; HIPPOCAMPUS; INTRACELLULAR FLUID; MALE; NEURONS; OPTICAL IMAGING; ORGAN CULTURE TECHNIQUES; RATS; TIME FACTORS;

EID: 84890843250     PISSN: 01650270     EISSN: 1872678X     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2013.11.021     Document Type: Article

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