Lipopolysaccharides reduce adipogenesis in 3T3-L1 adipocytes through activation of NF-κB pathway and downregulation of AMPK expression

Cardiovascular Toxicology

Volumn 13, Issue 4, 2013, Pages 338-346

  Wang, Lifeng ^a   Li, Linlin ^a   Ran, Xinjian ^a   Long, Mei ^a   Zhang, Minfang ^a   Tao, Yicun ^a   Luo, Xin ^a   Wang, Ye ^a   Jiao, Yi ^a   Mao, Xinmin ^a   Ren, Jun ^b  

^a XINJIANG MEDICAL UNIVERSITY   (China)

^b UNIVERSITY OF WYOMING COLLEGE OF HEALTH SCIENCES   (United States)

Author keywords

Adipogenesis; AMPK; I B ; Lipopolysaccharides; PPAR

Indexed keywords

BIOLOGICAL MARKER; CCAAT ENHANCER BINDING PROTEIN ALPHA; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE KINASE; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; LIPOPOLYSACCHARIDE; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA; PROTEIN AP2; UNCLASSIFIED DRUG;

ADIPOCYTE; ADIPOGENESIS; ARTICLE; CELL DIFFERENTIATION; CELL MATURATION; CELL VIABILITY; CONTROLLED STUDY; DOWN REGULATION; INHIBITION KINETICS; PRIORITY JOURNAL; PROADIPOCYTE; PROTEIN DEGRADATION; PROTEIN DNA BINDING; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTEIN STABILITY; SIGNAL TRANSDUCTION;

NEGIBACTERIA;

3T3-L1 CELLS; ADIPOCYTES; ADIPOGENESIS; AMP-ACTIVATED PROTEIN KINASES; ANIMALS; CELL SURVIVAL; DOWN-REGULATION; GENE EXPRESSION REGULATION, ENZYMOLOGIC; LIPOPOLYSACCHARIDES; MICE; NF-KAPPA B; SIGNAL TRANSDUCTION; UP-REGULATION;

EID: 84890555675     PISSN: 15307905     EISSN: 15590259     Source Type: Journal    
DOI: 10.1007/s12012-013-9214-2     Document Type: Article

References (26)

1. Cynthia, L.; Margaret, D.; Brian, K.; et al. (2012). Prevalence of obesity in the United States, 2009-2010. NCHS Data Brief, 82, 1-8.
2. Montminy, M.; & Koo, S. H. (2004). Diabetes: Outfoxing insulin resistance? Nature, 432, 958-959.
3. Cao, Y. (2010). Adipose tissue angiogenesis as a therapeutic target for obesity and metabolic diseases. Nature Reviews Drug Discovery, 9, 107-115.
4. Tang, Q. Q.; Otto, T. C.; & Lane, M. D. (2003). Mitotic clonal expansion: A synchronous process required for adipogenesis. Proceedings of the National Academy of Sciences of the United States of America, 100, 44-49.
5. Tang, Q. Q.; Otto, T. C.; & Lane, M. D. (2003). CCAAT/enhancer- binding protein beta is required for mitotic clonal expansion during adipogenesis. Proceedings of the National Academy of Sciences of the United States of America, 100, 850-855.
6. Tang, Q. Q.; Gronborg, M.; Huang, H.; et al. (2005). Sequential phosphorylation of CCAAT enhancer-binding protein beta by MAPK and glycogen synthase kinase 3beta is required for adipogenesis. Proceedings of the National Academy of Sciences of the United States of America, 102, 9766-9771.
7. Shimizu, K.; Ogura, H.; Hamasaki, T.; et al. (2010). Altered gut flora are associated with septic complications and death in critically ill patients with systemic inflammatory response syndrome. Digestive Diseases and Sciences, 56, 1171-1177.
8. Manco, M.; Putignani, L.; & Bottazzo, G. F. (2010). Gut microbiota, lipopolysaccharides, and innate immunity in the pathogenesis of obesity and cardiovascular risk. Endocrine Reviews, 31, 817-844.
9. Cani, P. D.; Bibiloni, R.; Knauf, C.; et al. (2008). Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice. Diabetes, 57, 1470-1481.
10. Muccioli, G. G.; Naslain, D.; Backhed, F.; et al. (2010). The endocannabinoid system links gut microbiota to adipogenesis. Molecular systems biology, 6, 392.
11. Poulain-Godefroy, Odile.; et al. (2008). Inflammation is associated with a decrease of lipogenic factors in omental fat in women. American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, 295, R1-R7.
12. Miranda, N.; Tovar, A. R.; Palacios, B.; & Torres, N. (2007). AMPK as a cellular energy sensor and its function in the organism. Revista de Investigacion Clinica, 59, 458-469.
13. Gao, Y.; Zhou, Y.; Xu, A.; & Wu, D. (2008). Effects of an AMP-activated protein kinase inhibitor, compound C, on adipogenic differentiation of 3T3-L1 cells. Biological and Pharmaceutical Bulletin, 31, 1716-1722.
14. Susan, A.; & Lee, A. (2001). The effects of AICAR on adipocyte differentiation of 3T3-L1 cells. Biochemical and Biophysical Research Communications, 286, 852-856.
15. Lira, F. S.; Rosa, J. C.; et al. (2012). Both adiponectin and interleukin-10 inhibit LPS-induced activation of the NF-κB pathway in 3T3-L1 adipocytes. Cytokine, 57, 98-106.
16. Karin, M.; Yamamoto, Y.; & Wang, Q. M. (2004). The IKK NF-κB system: A treasure trove for drug development. Nature Review, 3, 17-26.
17. Yarmo, M.; Gagnon, A.; & Sorisky, A. (2010). Anti-adipogenic effect of macrophage-conditioned medium requires the IKKβ/NF-κB pathway. Hormone and Metabolic Research, 42, 831-836.
18. Lu, B.; Lu, Y.; Moser, A. H.; Shigenaga, J. K.; Grunfeld, C.; & Feingold, K. R. (2008). LPS and proinflammatory cytokines decrease lipin-1 in mouse adipose tissue and 3T3-L1 adipocytes. American Journal of Physiology-Endocrinology and Metabolism, 295, E1502-E1509.
19. Rodriguez-Calvo, R.; Serrano, L.; Coll, T.; et al. (2008). Activation of peroxisome proliferator-activated receptor beta/delta inhibits lipopolysaccharide-induced cytokine production in adipocytes by lowering nuclear factor-kappaB activity via extracellular signal-related kinase 1/2. Diabetes, 57, 2149-2157.
20. Gustafson, B.; Gogg, S.; Hedjazifar, S.; Jenndahl, L.; Hammarstedt, A.; & Smith, U. (2009). Inflammation and impaired adipogenesis in hypertrophic obesity in man. American Journal of Physiology-Endocrinology and Metabolism, 297, E999-E1003.
21. Yamada, E.; Lee, T. W.; Pessin, J. E.; et al. (2010). Targeted therapies of the LKB1/AMPK pathway for the treatment of insulin resistance. Future Medicinal Chemistry, 2, 1785-1796.
22. Daval, M. (2006). Functions of AMP-activated protein kinase in adipose tissue. Journal of Physiology, 5741, 55-62.
23. Lee, H.; Kang, R.; Bae, S.; et al. (2011). (2011). AICAR, an activator of AMPK, inhibits adipogenesis via the WNT/β-catenin pathway in 3T3-L1 adipocytes. International Journal of Molecular Medicine, 28, 65-71.
24. Cavuoto, P.; McAinch, A. J.; et al. (2007). Effects of cannabinoid receptors on skeletal muscle oxidative pathways. Molecular and Cellular Endocrinology, 267, 63-69.
25. Suzawa, M.; Takada, I.; Yanagisawa, J.; et al. (2003). Cytokines suppress adipogenesis and PPAR-γ function through the TAK1/TAB 1/NIK cascade. Nature Cell Biology, 5, 224-230.
26. Kunos, G. (2007). Understanding metabolic homeostasis and imbalance: What is the role of the endocannabinoid system? American Journal of Medicine, 120, S18-S24.