Structural analysis of guanylyl cyclase-activating protein-2 (GCAP-2) homodimer by stable isotope-labeling, chemical cross-linking, and mass spectrometry

Journal of the American Society for Mass Spectrometry

Volumn 24, Issue 12, 2013, Pages 1969-1979

  Pettelkau, Jens ^a   Thondorf, Iris ^a   Theisgen, Stephan ^b   Lilie, Hauke ^a   Schröder, Thomas ^a,c   Arlt, Christian ^a   Ihling, Christian H ^a   Sinz, Andrea ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^b UNIVERSITY OF LEIPZIG   (Germany)

^c NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Analytical ultracentrifugation; Chemical cross linking; Dimerization; GCAP 2; Isotope labeling; Mass spectrometry; Protein 3D structure

Indexed keywords

ANALYTICAL ULTRACENTRIFUGATION; CHEMICAL CROSS-LINKING; GCAP-2; HIGH RESOLUTION MASS SPECTROMETRY; ISOTOPE LABELING; ISOTOPE-LABELED PROTEIN; STRUCTURAL INFORMATION; UNAMBIGUOUS DISCRIMINATION;

CALCIUM; CENTRIFUGATION; DIMERIZATION; ISOTOPES; MASS SPECTROMETRY; MOLECULAR DYNAMICS; PROTEINS; SIZE EXCLUSION CHROMATOGRAPHY;

CROSSLINKING;

CALCIUM BINDING PROTEIN; CALCIUM ION; GUANYLATE CYCLASE ACTIVATING PROTEIN 2; HOMODIMER; MONOMER; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; CROSS LINKING; GEL PERMEATION CHROMATOGRAPHY; ISOTOPE LABELING; MASS SPECTROMETRY; MOLECULAR DOCKING; MOLECULAR DYNAMICS; PROTEIN ASSEMBLY; STRUCTURE ANALYSIS; TANDEM MASS SPECTROMETRY; ULTRACENTRIFUGATION;

ANIMALS; CALCIUM; CATTLE; CROSS-LINKING REAGENTS; GUANYLATE CYCLASE-ACTIVATING PROTEINS; ISOTOPE LABELING; MOLECULAR DOCKING SIMULATION; NITROGEN ISOTOPES; PROTEIN CONFORMATION; PROTEIN MULTIMERIZATION; TANDEM MASS SPECTROMETRY;

EID: 84890525898     PISSN: 10440305     EISSN: 18791123     Source Type: Journal    
DOI: 10.1007/s13361-013-0734-6     Document Type: Article

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