Duplex real-Time PCR for rapid simultaneous detection of Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans in amphibian samples

Journal of Clinical Microbiology

Volumn 51, Issue 12, 2013, Pages 4173-4177

  Blooi, M ^a   Pasmans, F ^a   Longcore, J E ^b   Spitzen Van Der Sluijs, A ^c   Vercammen, F ^d   Martel, A ^a  

^a GHENT UNIVERSITY   (Belgium)

^b UNIVERSITY OF MAINE   (United States)

^c RAVON   (Netherlands)

^d UNIVERSITY OF ANTWERP   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL DNA;

AMPHIBIA; ANIMAL TISSUE; ANURA; ARTICLE; BATRACHOCHYTRIUM; BATRACHOCHYTRIUM DENDROBATIDIS; BATRACHOCHYTRIUM SALAMANDRIVORANS; CONTROLLED STUDY; DUPLEX REAL TIME POLYMERASE CHAIN REACTION; FUNGAL DETECTION; FUNGUS CULTURE; GENE AMPLIFICATION; LIMIT OF DETECTION; MOLECULAR PROBE; NONHUMAN; NUCLEIC ACID ANALYSIS; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RELIABILITY; REPRODUCIBILITY; SALAMANDER; SENSITIVITY AND SPECIFICITY; ZOOSPORE;

AMPHIBIANS; ANIMALS; CHYTRIDIOMYCOTA; MULTIPLEX POLYMERASE CHAIN REACTION; MYCOSES; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 84889873224     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.02313-13     Document Type: Article

References (16)

1. Stuart SN, Chanson JS, Cox NA, Young BE, Rodrigues ASL, Fischman DL, Waller RW. 2004. Status and trends of amphibian declines and extinctions worldwide. Science 306:1783-1786.
2. Daszak P, Berger L, Cunningham AA, Hyatt AD, Green DE, Speare R. 1999. Emerging infectious diseases and amphibian population declines. Emerg. Infect. Dis. 5:735-748.
3. Skerratt LF, Berger L, Speare R, Cashins S, McDonald KR, Phillott AD, Hines HB, Kenyon N. 2007. Spread of chytridiomycosis has caused the rapid global decline and extinction of frogs. EcoHealth 4:125-134.
4. Longcore JE, Pessier AP, Nichols DK. 1999. Batrachochytrium dendrobatidis gen. et sp. nov., a chytrid pathogenic to amphibians. Mycologia 91:219-227.
5. Spitzen-van der Sluijs A, Spikmans F, Bosman W, De Zeeuw M, van der Meij T, Goverse E, Kik M, Pasmans F, Martel A. 2013. Rapid enigmatic decline drives the fire salamander (Salamandra salamandra) to the edge of extinction in the Netherlands. Amphibia-Reptilia 34:233-239.
6. Martel A, Spitzen-van der Sluijs A, Blooi M, Bert W, Ducatelle R, Fisher MC, Woeltjes A, Bosman W, Chiers K, Bossuyt F, Pasmans F. 2013. Batrachochytrium salamandrivorans sp. nov. causes lethal chytridiomycosis in amphibians. Proc. Natl. Acad. Sci. U. S. A. 110:15325-15329.
7. Annis SL, Dastoor FP, Ziel H, Daszak P, Longcore JE. 2004. A DNAbased assay identifies Batrachochytrium dendrobatidis in amphibians. J. Wildl. Dis. 40:420-428.
8. Boyle DG, Boyle DB, Olsen V, Morgan JAT, Hyatt AD. 2004. Rapid quantitative detection of chytridiomycosis (Batrachochytrium dendrobatidis) in amphibian samples using real-Time Taqman PCR assay. Dis. Aquat. Organ. 60:141-148.
9. Hyatt AD, Boyle DG, Olsen V, Boyle DB, Berger L, Obendorf D, Dalton A, Kriger K, Hero M, Hines H, Phillott R, Campbell R, Marantelli G, Gleason F, Colling A. 2007. Diagnostic assays and sampling protocols for the detection of Batrachochytrium dendrobatidis. Dis. Aquat. Organ. 73: 175-192.
10. Garland S, Baker A, Phillott AD, Skerratt LF. 2010. BSA reduces inhibition in a TaqMan (R) assay for the detection of Batrachochytrium dendrobatidis. Dis. Aquat. Organ. 92:113-116.
11. Kreader CA. 1996. Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein. Appl. Environ. Microbiol. 62:1102-1106.
12. Van Rooij P, Martel A, Nerz J, Voitel S, Van Immerseel F, Haesebrouck F, Pasmans F. 2011. Detection of Batrachochytrium dendrobatidis in Mexican bolitoglossine salamanders using an optimal sampling protocol. Eco- Health 8:237-243.
13. Bandi C, Damiani G, Magrassi L, Grigolo A, Fani R, Sacchi L. 1994. Flavobacteria as intracellular symbionts in cockroaches. Proc. Biol. Sci. 257:43-48.
14. Kirshtein JD, Anderson CW, Wood JS, Longcore JE, Voytek MA. 2007. Quantitative PCR detection of Batrachochytrium dendrobatidisDNAfrom sediments and water. Dis. Aquat. Organ. 77:11-15.
15. Longo AV, Rodriguez D, da Silva Leite D, Toledo LF, Mendoza Almeralla C, Burrowes PA, Zamudio KR. 2013. ITS1 copy number varies among Batrachochytrium dendrobatidis strains: implications for qPCR estimates of infection intensity from field-collected amphibian skin swabs. PLoS One 8(3):e59499. doi:10.1371/journal.pone.0059499.
16. Vredenburg VT, Knapp RA, Tunstall TS, Briggs CJ. 2010. Dynamics of an emerging disease drive large-scale amphibian population extinctions. Proc. Natl. Acad. Sci. U. S. A. 107:9689-9694.