BSA reduces inhibition in a TaqMan® assay for the detection of Batrachochytrium dendrobatidis

Diseases of Aquatic Organisms

Volumn 92, Issue 2-3, 2010, Pages 113-116

  Garland, Stephen ^a   Baker, Anthony ^a   Phillott, Andrea D ^a   Skerratt, Lee F ^a  

^a JAMES COOK UNIVERSITY   (Australia)

Author keywords

Batrachochytrium dendrobatidis; Bovine serum albumin; BSA; Chytridiomycosis; Inhibition; Quantitative PCR; TaqMan assay

Indexed keywords

BOVINE SERUM ALBUMIN; PHENOL DERIVATIVE; TANNIN DERIVATIVE;

AMPHIBIAN; BIOASSAY; FUNGUS; HUMIC ACID; INHIBITION; PHENOLIC COMPOUND; POLYMERASE CHAIN REACTION; PROTEIN; QUANTITATIVE ANALYSIS; WILD POPULATION;

ANIMAL; ANIMAL DISEASE; ARTICLE; CHEMISTRY; CHYTRIDIOMYCETES; FROG; GENETICS; HUMIC SUBSTANCE; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; MYCOSIS; POLYMERASE CHAIN REACTION;

ANIMALS; CHYTRIDIOMYCOTA; HUMIC SUBSTANCES; MYCOSES; PHENOLS; POLYMERASE CHAIN REACTION; RANIDAE; SERUM ALBUMIN, BOVINE; TANNINS;

AMPHIBIA; BATRACHOCHYTRIUM DENDROBATIDIS; BOVINAE;

EID: 78650478273     PISSN: 01775103     EISSN: 16161580     Source Type: Journal    
DOI: 10.3354/dao02053     Document Type: Article

References (4)

1. Boyle DG, Boyle DB, Olsen V, Morgan JAT, Hyatt AD (2004) Rapid quantitative detection of chytridiomycosis (Batrachochytrium dendrobatidis) in amphibian samples using real-time Taqman PCR assay. Dis Aquat Org 60:141-148
2. Hyatt AD, Boyle DG, Olsen V, Boyle DB and others (2007) Diagnostic assays and sampling protocols for the detection of Batrachochytrium dendrobatidis. Dis Aquat Org 73: 175-192
3. Kreader CA (1996) Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein. Appl Environ Microbiol 62:1102-1106
4. Skerratt LF, Berger L, Speare R, Cashins S and others (2007) Spread of chytridiomycosis has caused the rapid global decline and extinction of frogs. EcoHealth 4:125-134