Chemically-Defined Culture of Human Embryonic Stem Cells

Human Embryonic Stem Cells: The Practical Handbook

Volumn , Issue , 2007, Pages 81-90

  Clark, Julie Hsu ^a   Ding, Sheng ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

CDM; Directed differentiation; Functional genomics; High throughput screen; Human embryonic stem cells chemically defined medium; Self renewal

Indexed keywords

EID: 84889406853     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9780470511619.ch6     Document Type: Chapter

References (11)

1. James D, AJ Levine, D Besser and A Hemmati-Brivanlou. (2005). TGFbeta/activin/nodal signaling is necessary for the maintenance of pluripotency in human embryonic stem cells. Development 132(6): 1273-1282.
2. Kleinman HK, ML McGarvey, LA Liotta, PG Robey, K Tryggvason and GR Martin. (1982). Isolation and characterization of type IV procollagen, laminin, and heparan sulfate proteoglycan from the EHS sarcoma. Biochemistry 21: 6188-6193.
3. Lu J, R Hou, CJ Booth, SH Yang and M Snyder. (2006). Defined culture conditions of human embryonic stem cells. Proc Natl Acad Sci USA 103: 5688-5693.
4. Ludwig TE, ME Levenstein, JM Jones, WT Berggren, ER Mitchen, JL Frane, LJ Crandall, CA Daigh, KR Conard, MS Piekarczyk, RA Llanas and JA Thomson. (2006). Derivation of human embryonic stem cells in defined conditions. Nat Biotechnol 24: 185-187.
5. Martin MJ, A Muotri, F Gage and A Varki. (2005). Human embryonic stem cells express an immunogenic nonhuman sialic acid. Nat Med 11: 228-232.
6. Vallier L, M Alexander and RA Pedersen. (2005). Activin/Nodal and FGF pathways cooperate to maintain pluripotency of human embryonic stem cells. J Cell Sci 118: 4495-4509.
7. Vukicevic S, HK Kleinman, FP Luyten, AB Roberts, NS Roche and AH Reddi. (1992). Identification of multiple active growth factors in basement membrane Matrigel suggests caution in interpretation of cellular activity related to extracellular matrix components. Exp Cell Res 202: 1-8.
8. Wang G, H Zhang, Y Zhao, J Li, J Cai, P Wang, S Meng, J Feng, C Miao, M Ding, D Li and H Deng. (2005). Noggin and bFGF cooperate to maintain the pluripotency of human embryonic stem cells in the absence of feeder layers. Biochem Biophys Res Commun 330: 934-942.
9. Xu RH, X Chen, DS Li, R Li, GC Addicks, C Glennon, TP Zwaka and JA Thomson. (2002). BMP4 initiates human embryonic stem cell differentiation to trophoblast. Nat Biotechnol 20: 1261-1264.
10. Xu RH, RM Peck, DS Li, X Feng, T Ludwig and JA Thomson. (2005). Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells. Nat Methods 2: 185-190.
11. Yao S, S Chen, J Clark, E Hao, GM Beattie, A Hayek and S Ding. (2006). Long-term selfrenewal and directed differentiation of human embryonic stem cells in chemically defined conditions. Proc Natl Acad Sci USA. 103: 6907-6912.