Detection of Cryptosporidium and Giardia (oo)cysts by IFA, PCR and LAMP in surface water from Rasht, Iran

Transactions of the Royal Society of Tropical Medicine and Hygiene

Volumn 107, Issue 8, 2013, Pages 511-517

  Mahmoudi, Mohammad Reza ^a,b,c   Kazemi, Bahram ^b   Mohammadiha, Anita ^b   Mirzaei, Asad ^d   Karanis, Panagiotis ^c  

^a GUILAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^b SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^c UNIVERSITY OF COLOGNE   (Germany)

^d Department of Parasitology School of Medicine Iilam University of Medical Sciences   (Iran)

Author keywords

Cryptosporidium; Giardia; Iran; LAMP; PCR; Water

Indexed keywords

GLUTAMATE DEHYDROGENASE; SURFACE WATER;

ARTICLE; CONTROLLED STUDY; CRYPTOSPORIDIUM; GIARDIA; IMMUNOFLUORESCENCE TEST; IRAN; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; MEMBRANE FILTER; MICROORGANISM DETECTION; NONHUMAN; OOCYST; POLYMERASE CHAIN REACTION; RIVER; WATER ANALYSIS; WATER CONTAMINATION; WATER SUPPLY;

CRYPTOSPORIDIUM; GIARDIA; IRAN; LAMP; PCR; WATER;

ANIMALS; CRYPTOSPORIDIUM; FILTRATION; FLUORESCENT ANTIBODY TECHNIQUE; GIARDIA; HUMANS; IRAN; NUCLEIC ACID AMPLIFICATION TECHNIQUES; OOCYSTS; POLYMERASE CHAIN REACTION; RIVERS; WATER POLLUTION;

EID: 84889047835     PISSN: 00359203     EISSN: 18783503     Source Type: Journal    
DOI: 10.1093/trstmh/trt042     Document Type: Article

References (32)

1. Karanis P, Kourenti C, Smith H. Waterborne transmission of protozoanparasites: a worldwide review of outbreaks and lessons learnt. J WatHealth 2007;5:1-38.
2. Baldursson S, Karanis P. Waterborne transmission of protozoanparasites: Review of worldwide outbreaks: an update 2004-2010.Wat Res 2011;45:6603-14.
3. LeChevallierMW, Di Giovanni GD, Clancy Jennifer L et al. Comparison of method 1623 and cell culture-PCR for detection of Cryptosporidium spp. in source waters. Appl Environ Microbiol 2003;69:971-9.
4. Plutzer J, Torokne A, Karanis P. Combination ofARADmicrofibre filtrationandLAMPmethodology for simple, rapidandcost-effectivedetection ofhuman pathogenic Giardia duodenalis and Cryptosporidium spp. indrinking water. Lett Appl Microbiol 2009;50:82-8.
5. Plutzer J, Karanis P, Domokos K et al. Detection and characterisation ofGiardia and Cryptosporidium in Hungarian raw, surface and sewagewater samples by IFT, PCR and sequence analysis of the SSUrRNA andGDH genes. Int J Hyg Environ Health 2008;211:524-33.
6. Karanis P, Thekisoe O, Kiouptsi K et al. Development and preliminaryevaluation of a loop-mediated isothermal amplification procedure forsensitive detection of Cryptosporidium oocysts in fecal and watersamples. Appl Environ Microbiol 2007;73:5660-2.
7. Bakheit MA, Torra D, Palomino LA et al. Sensitive and specific detectionof Cryptosporidium species in PCR-negative samples by loop-mediatedisothermal DNA amplification and confirmation of generated LAMPproducts by sequencing. Vet Parasitol 2008;158:11-22.
8. Plutzer J, Karanis P. Rapid identification of Giardia duodenalis byloop-mediated isothermal amplification (LAMP) from fecal andenvironmental samples and comparative findings by PCR andReal-Time PCR methods. Parasitol Res 2009;104:1527-33.
9. Xiao L, Alderisio K, Limor J et al. Identification of species and sources ofCryptosporidium oocysts in storm waters with a small-subunitrRNA-based diagnostic and genotyping tool. Appl Environ Microbiol2000;66:5492-8.
10. Read CM, Morris PT, Thompson RCA. Discrimination of all genotypes ofGiardia duodenalis at the glutamate dehydrogenase locus usingPCR-RFLP. Infect Genet Evol 2004;4:125-30.
11. Johnson DW, Pieniazek NJ, Griffin DW et al. Development of a PCRprotocol for sensitive detection of Cryptosporidium oocysts in watersamples. Appl Environ Microbiol 1995;61:3849-55.
12. Carmena D, Aguinagalde X, Zigorraga C et al. Presence of Giardia cystsand Cryptosporidium oocysts in drinking water supplies in NorthernSpain. J Appl Microbiol 2007;102:619-29.
13. Mayer CL, Palmer CJ. Evaluation of PCR, nested PCR, and fluorescentantibodies for detection of Giardia and Cryptosporidium species inwastewater. Appl Environ Microbiol 1996;62:2081-5.
14. Robertson LJ, Gjerde B. Occurrence of Cryptosporidium oocysts andGiardia cysts in raw waters in Norway. Scand J Public Health2001;29:200-7.
15. Hanninen ML, Horman A, Rimhanen-Finne R et al. Monitoring ofCryptosporidium and Giardia in the Vantaa river basin, southernFinland. Int J Hyg Environ Health 2005;208:163-71.
16. Medema GJ, Schijven JF. Modelling the sewage discharge anddispersion of Cryptosporidium and Giardia in surface water. Water Res2001;35:4307-16.
17. Briancesco R, Bonadonna L. An Italian study on Cryptosporidium andGiardia in wastewater, fresh water and treated water. Environ MonitAssess 2005;104:445-57.
18. Bodley-Tickell AT, Kitchen SE, Sturdee AP. Occurrence ofCryptosporidium in agricultural surface waters during an annualfarming cycle in lowland UK.Water Res 2002;36:1880-6.
19. Karanis P, Sotiriadou I, Kartashev V et al. Occurrence of Giardia andCryptosporidium in water supplies of Russia and Bulgaria. Environ Res2006;102:260-71.
20. Jiang J, Alderisio KA, Singh A, Xiao L. Development of proceduresfor direct extraction of Cryptosporidium DNA from water concentratesand for relief of PCR inhibitors. Appl Environ Microbiol2005;71:1135-41.
21. Nichols RAB, Campbell BM, Smith HV. Identification of Cryptosporidiumspp. oocysts in U.K. still natural mineral waters and drinking watersusing a modified nested PCR-RFLP assay. Appl Environ Microbiol2003;69:4183-9.
22. SmithHV, Nichols RA. Cryptosporidium: detection inwaterand food. ExpParasitol 2010;124:61-79.
23. Xiao L, Singh A, Limor J et al. Molecular characterization ofCryptosporidium oocysts in samples of raw surface water andwastewater. Appl Environ Microbiol 2001;67:1097-101.
24. Kothavade R. Potential molecular tools for assessing the public healthrisk associated with waterborne Cryptosporidium oocysts. J MedMicrobiol 2012;6:1039-51.
25. Lebbad M, Petersson I, Karlsson L et al. Multilocus genotyping ofhumanGiardia isolates suggests limited zoonotic transmission and associationbetween assemblage B and flatulence in children. PLoS Negl Trop Dis.2011;5:e1262.
26. Ozkul IA, Aydin Y. Natural Cryptosporidium muris infection of thestomach in laboratory mice. Vet Parasitol 1994;55:129-32.
27. Sotiriadou I, Karanis P. Evaluation of loop-mediated isothermalamplification for detection of Toxoplasma gondii in water samplesand comparative findings by polymerase chain reaction andimmunofuorescence test. Diagn Microbiol Infect Dis 2008;62:357-65.
28. Keshvarz A, Athari A, Haghighi A et al. Genetic characterization ofCryptosporidium spp. among children with diarrhea in Tehran andQazvin provinces. Iran Iranian J Parasitol 2008;3:33-6.
29. Nazemalhosseini-Mojarad E, Haghighib A, Taghipour N et al.Subtype analysis of Cryptosporidium parvum and Cryptosporidiumhominis isolates from humans and cattle in Iran. Vet Parasitol2011;179:250-2.
30. Keshavarz A, Haghighi A, Athari A et al. Prevalence and molecularcharacterization of bovine Cryptosporidium in Qazvin province, Iran.Vet Parasitol 2009;160:316-8.
31. Sayyari AA, Imanzadeh F, Bagheri Yazdi SA, Karami HYM. Prevalence ofintestinal parasitic infections in the Islamic Republic of Iran. EastMediterr Health J 2009;11:377-83.
32. HatamNahavandi K, Fallah E, Jamali R et al. Genetic characterization ofGiardia intestinalis strains from patients having sporadic giardiasis byusing PCR assay. J Med Sci 2008;8:310-5.