Imaging microglia in brain slices and slice cultures

Cold Spring Harbor Protocols

Volumn 2013, Issue 12, 2013, Pages 1142-1148

  Dailey, Michael E ^a   Eyo, Ukpong ^a   Fuller, Leah ^a   Hass, John ^a   Kurpius, Dana ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; LECTIN; YELLOW FLUORESCENT PROTEIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN SLICE; BRAIN TISSUE; CELL MIGRATION; CELL MOTILITY; CELL PHAGOCYTOSIS; CELL PROLIFERATION; CENTRAL NERVOUS SYSTEM; CHEMOTAXIS; CONFOCAL MICROSCOPY; FLUORESCENCE MICROSCOPY; HIPPOCAMPUS; IMAGING SOFTWARE; IMAGING SYSTEM; MICROGLIA; MOUSE; NEWBORN; NONHUMAN; RAT; TISSUE CULTURE; ADULT; CHEMOKINESIS; GENE EXPRESSION; MICROGLIAL CELL CULTURE; NEUROIMAGING; TIME LAPSE IMAGING;

ANIMALS; BRAIN; GENES, REPORTER; MICE; MICE, TRANSGENIC; MICROGLIA; MICROSCOPY, FLUORESCENCE, MULTIPHOTON; OPTICAL IMAGING; STAINING AND LABELING; TIME-LAPSE IMAGING;

EID: 84888999312     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot079483     Document Type: Article

References (12)

1. Dailey ME., Waite M. 1999. Confocal imaging of microglial cell dynamics in hippocampal slice cultures. Methods 18: 222-230.
2. Dailey ME., Marrs GS, Kurpius D. 2011. Maintaining live cells and tissue slices in the imaging setup. In Imaging in Neuroscience: A Laboratory Manual (ed. Helmchen Konnerth), pp. 331-338. CSHL Press, Cold Spring Harbor, NY.
3. Fuller L., Dailey ME. 2007. Preparation of rodent hippocampal slice cultures. Cold Spring Harb Protoc doi: 10.1101/pdb.prot4848.
4. Haynes SE., Hollopeter G, Yang G, Kurpius D, Dailey ME, Gan W-B, Julius D. 2006. The P2Y12 receptor regulates microglial activation by extracellular nucleotides. Nat Neurosci 9: 1512-1519.
5. Hirasawa T., Ohsawa K, Imai Y, Ondo Y, Akazawa C, Uchino S, Kohsaka S. 2005. Visualization of microglia in living tissues using Iba1-EGFP transgenic mice. J Neurosci Res 81: 357-362.
6. Jung S., Aliberti J, Graemmel P, Sunshine MJ, Kreutzberg GW, Sher A, Littman DR. 2000. Analysis of fractalkine receptor CX3CR1 function by targeted deletion and green fluorescent protein reporter gene insertion. Mol Cell Biol 20: 4106-4114.
7. Kurpius D., Wilson N, Fuller L, Hoffman A, Dailey ME. 2006. Early activation, motility, and homing of neonatal microglia to injured neurons does not require protein synthesis. Glia 54: 58-70.
8. Kurpius D., Nolley EP, Dailey ME. 2007. Purines induce directed migration and rapid homing of microglia to injured pyramidal neurons in developing hippocampus. Glia 55: 873-884.
9. Petersen M., Dailey ME. 2004. Diverse microglial motility behaviors during clearance of dead cells in hippocampal slices. Glia 46: 195-206.
10. Stence N., Waite M, Dailey ME. 2001. Dynamics of microglial activation: A confocal time-lapse analysis in hippocampal slices. Glia 33: 256-266.
11. Stoppini L., Buchs P-A, Muller D. 1991. A simple method for organotypic cultures of nervous tissue. J Neurosci Methods 37: 173-182.
12. Streit WJ., Kreutzberg GW. 1987. Lectin binding by resting and reactive microglia. J Neurocytol 16: 249-260.