Functional expression of FIP-fve, a fungal immunomodulatory protein from the edible mushroom Flammulina velutipes in Pichia pastoris GS115

Journal of Biotechnology

Volumn 168, Issue 4, 2013, Pages 527-533

  Lin, Jing Wei ^a   Jia, Jia ^a   Shen, Yu Hua ^b   Zhong, Ming ^a   Chen, Li Jing ^a   Li, Hao Ge ^a   Ma, Hui ^a   Guo, Zhi Fu ^a   Qi, Ming Fang ^a   Liu, Li Xia ^c   Li, Tian Lai ^a  

^a SHENYANG AGRICULTURAL UNIVERSITY   (China)

^b Chifeng University   (China)

^c NORTHEAST NORMAL UNIVERSITY   (China)

Author keywords

Chromatography; Flammulina velutipes; Fungal immunomodulatory protein; Recombinant proteins; Shake flask

Indexed keywords

AMMONIUM SULFATE PRECIPITATION; ANTI-TUMOR ACTIVITIES; FLAMMULINA VELUTIPES; FUNCTIONAL EXPRESSION; IMMUNOMODULATORY; IN-VITRO BIOACTIVITY; PICHIA PASTORIS GS115; SHAKE-FLASK;

BIOACTIVITY; BIOTECHNOLOGY; CHROMATOGRAPHY; CLONING; GENES; ION EXCHANGE; MEDICAL APPLICATIONS; RECOMBINANT PROTEINS; YEAST;

PROTEINS;

AMMONIUM SULFATE; FUNGAL IMMUNOMODULATORY PROTEIN FVE; FUNGAL PROTEIN; GAMMA INTERFERON; IMMUNOMODULATING AGENT; INTERLEUKIN 2; RECOMBINANT PROTEIN; SEPHADEX; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTINEOPLASTIC ACTIVITY; ARTICLE; BIOLOGICAL ACTIVITY; CELL VIABILITY; CONTROLLED STUDY; CYTOKINE RELEASE; FLAMMULINA VELUTIPES; GEL CHROMATOGRAPHY; HEMAGGLUTINATION TEST; HUMAN; HUMAN CELL; IMMUNOMODULATION; MOLECULAR CLONING; MOUSE; NONHUMAN; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; SPLEEN CELL;

BASIDIOMYCOTA; FLAMMULINA VELUTIPES; MURINAE; PICHIA PASTORIS;

CHROMATOGRAPHY; FLAMMULINA VELUTIPES; FUNGAL IMMUNOMODULATORY PROTEIN; RECOMBINANT PROTEINS; SHAKE-FLASK;

ANIMALS; ERYTHROCYTES; FLAMMULINA; FUNGAL PROTEINS; GENE EXPRESSION REGULATION; HUMANS; INTERFERON-GAMMA; INTERLEUKIN-2; LYMPHOCYTES; MICE; PICHIA; RECOMBINANT PROTEINS;

EID: 84888837167     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2013.09.013     Document Type: Article

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