Down-regulation of Wnt10a by RNA interference inhibits proliferation and promotes apoptosis in mouse embryonic palatal mesenchymal cells through Wnt/β-catenin signaling pathway

Journal of Physiology and Biochemistry

Volumn 69, Issue 4, 2013, Pages 855-863

  Feng, Cuijuan ^a   Xu, Zhongfei ^a   Li, Zengjian ^a   Zhang, Dan ^a   Liu, Qiang ^a   Lu, Li ^a  

^a CHINA MEDICAL UNIVERSITY   (China)

Author keywords

Apoptosis; Cleft palate; Mouse embryonic palatal mesenchymal cell; Proliferation; RNA interference; Wnt

Indexed keywords

BETA CATENIN; MESSENGER RNA; SHORT HAIRPIN RNA; WNT10A PROTEIN;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL CYCLE G1 PHASE; CELL PROLIFERATION; CHROMATIN; DOWN REGULATION; GENE SILENCING; MESENCHYME CELL; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; RNA INTERFERENCE; S PHASE CELL CYCLE CHECKPOINT; SIGNAL TRANSDUCTION; WESTERN BLOTTING;

ANIMALS; APOPTOSIS; BETA CATENIN; CELL PROLIFERATION; EMBRYO, MAMMALIAN; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENE KNOCKDOWN TECHNIQUES; MESENCHYMAL STROMAL CELLS; MICE; MICE, INBRED C57BL; NERVE TISSUE PROTEINS; PALATE; PRIMARY CELL CULTURE; RNA, SMALL INTERFERING; S PHASE CELL CYCLE CHECKPOINTS; SIGNAL TRANSDUCTION; WNT PROTEINS;

EID: 84888428447     PISSN: 11387548     EISSN: 18778755     Source Type: Journal    
DOI: 10.1007/s13105-013-0262-7     Document Type: Article

References (26)

1. Beaty TH, Hetmanski JB, Fallin MD, Park JW, Sull JW, McIntosh I, Liang KY, Vanderkolk CA, Redett RJ, Boyadjiev SA et al (2006) Analysis of candidate genes on chromosome 2 in oral cleft case-parent trios from three populations. Hum Genet 120:501-518
2. Cawthorn WP, Bree AJ, Yao Y, Du B, Hemati N, Martinez-Santibanez G, MacDougald OA (2012) Wnt6, Wnt10a and Wnt10b inhibit adipogenesis and stimulate osteoblastogenesis through a beta-catenin-dependent mechanism. Bone 50:477-489
3. Chiquet BT, Blanton SH, Burt A, Ma D, Stal S, Mulliken JB, Hecht JT (2008) Variation in WNT genes is associated with non-syndromic cleft lip with or without cleft palate. Hum Mol Genet 17:2212-2218
4. Christensen K, Juel K, Herskind AM, Murray JC (2004) Long term follow up study of survival associated with cleft lip and palate at birth. BMJ 328:1405
5. Dixon MJ, Marazita ML, Beaty TH, Murray JC (2011) Cleft lip and palate: understanding genetic and environmental influences. Nat Rev Genet 12:167-178
6. Han J, Xiao Y, Lin J, Li Y (2006) PDGF-C controls proliferation and is down-regulated by retinoic acid in mouse embryonic palatal mesenchymal cells. Birth Defects Res B Dev Reprod Toxicol 77:438-444
7. He F, Xiong W, Yu X, Espinoza-Lewis R, Liu C, Gu S, Nishita M, Suzuki K, Yamada G, Minami Y, Chen Y (2008) Wnt5a regulates directional cell migration and cell proliferation via Ror2-mediated noncanonical pathway in mammalian palate development. Development 135(23):3871-3879
8. Hsu RJ, Ho JY, Cha TL, Yu DS, Wu CL, Huang WP, Chu P, Chen YH, Chen JT, Yu CP (2012) WNT10A plays an oncogenic role in renal cell carcinoma by activating WNT/beta-catenin pathway. PLoS One 7:e47649
9. Hu X, Chen Z, Mao X, Tang S (2011) Effects of phenytoin and Echinacea purpurea extract on proliferation and apoptosis of mouse embryonic palatal mesenchymal cells. J Cell Biochem 112:1311-1317
10. Jin YR, Han XH, Taketo MM, Yoon JK (2012) Wnt9b-dependent FGF signaling is crucial for outgrowth of the nasal and maxillary processes during upper jaw and lip development. Development 139:1821-1830
11. Kagalwalla AF, Akhtar N, Woodruff SA, Rea BA, Masterson JC, Mukkada V, Parashette KR, Du J, Fillon S, Protheroe CA, Lee JJ, Amsden K, Melin-Aldana H, Capocelli KE, Furuta GT, Ackerman SJ (2012) Eosinophilic esophagitis: epithelial mesenchymal transition contributes to esophageal remodeling and reverses with treatment. J Allergy Clin Immunol 129(5):1387-1396.e7
12. Kokkinos MI, Wafai R, Wong MK, Newgreen DF, Thompson EW, Waltham M (2007) Vimentin and epithelial-mesenchymal transition in human breast cancer - observations in vitro and in vivo. Cells Tissues Organs 185(1-3):191-203
13. Lan Y, Ryan RC, Zhang Z, Bullard SA, Bush JO, Maltby KM, Lidral AC, Jiang R (2006) Expression of Wnt9b and activation of canonical Wnt signaling during midfacial morphogenesis in mice. Dev Dyn 235:1448-1454
14. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25:402-408
15. Menezes R, Letra A, Kim AH, Kuchler EC, Day A, Tannure PN, Gomes da Motta L, Paiva KB, Granjeiro JM, Vieira AR (2010) Studies with Wnt genes and nonsyndromic cleft lip and palate. Birth Defects Res A Clin Mol Teratol 88:995-1000
16. Meng L, Bian Z, Torensma R, Von den Hoff JW (2009) Biological mechanisms in palatogenesis and cleft palate. J Dent Res 88:22-33
17. Mossey P (2007) Epidemiology underpinning research in the aetiology of orofacial clefts. Orthod Craniofac Res 10:114-120
18. Mossey PA, Little J, Munger RG, Dixon MJ, Shaw WC (2009) Cleft lip and palate. Lancet 374:1773-1785
19. Ngai CW, Martin WL, Tonks A, Wyldes MP, Kilby MD (2005) Are isolated facial cleft lip and palate associated with increased perinatal mortality? A cohort study from the West Midlands Region, 1995-1997. J Matern Fetal Neonatal Med 17:203-206
20. Song L, Li Y, Wang K, Wang YZ, Molotkov A, Gao L, Zhao T, Yamagami T, Wang Y, Gan Q et al (2009) Lrp6-mediated canonical Wnt signaling is required for lip formation and fusion. Development 136:3161-3171
21. Tyler MS, Pratt RM (1980) Effect of epidermal growth factor on secondary palatal epithelium in vitro: tissue isolation and recombination studies. J Embryol Exp Morphol 58:93-106
22. Ueno S, Weidinger G, Osugi T, Kohn AD, Golob JL, Pabon L, Reinecke H, Moon RT, Murry CE (2007) Biphasic role for Wnt/beta-catenin signaling in cardiac specification in zebrafish and embryonic stem cells. Proc Natl Acad Sci U S A 104:9685-9690
23. Warner DR, Smith HS, Webb CL, Greene RM, Pisano MM (2009) Expression of Wnts in the developing murine secondary palate. Int J Dev Biol 53:1105-1112
24. Wehby GL, Cassell CH (2010) The impact of orofacial clefts on quality of life and healthcare use and costs. Oral Dis 16:3-10
25. Yao T, Yang L, Li PQ, Wu H, Xie HB, Shen X, Xie XD (2011) Association of Wnt3A gene variants with non-syndromic cleft lip with or without cleft palate in Chinese population. Arch Oral Biol 56:73-78
26. Zhu P, Chen G, You T, Yao J, Jiang Q, Lin X, Shen X, Qiao Y, Lin L (2010) High FFA-induced proliferation and apoptosis in human umbilical vein endothelial cell partly through Wnt/beta-catenin signal pathway. Mol Cell Biochem 338:123-131