A universal TaqMan-based RT-PCR protocol for cost-efficient detection of small noncoding RNA

RNA

Volumn 19, Issue 12, 2013, Pages 1864-1873

  Jung, Ulrike ^a,b   Jiang, Xiaoou ^c   Kaufmann, Stefan H E ^a   Patzel, Volker ^a,c  

^a MAX PLANCK INSTITUTE FOR INFECTION BIOLOGY   (Germany)

^b CITY OF HOPE NATIONAL MEDICAL CENTER   (United States)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Noncoding RNA detection; Real time PCR; Stem loop primer; TaqMan PCR

Indexed keywords

BACTERIAL RNA; MICRORNA; RNA INDUCED SILENCING COMPLEX; RNA PRECURSOR; SMALL UNTRANSLATED RNA;

ARTICLE; CONTROLLED STUDY; EXPERIMENTAL DESIGN; FEASIBILITY STUDY; HUMAN; HUMAN CELL; HUMAN TISSUE; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SAMPLE SIZE; SENSITIVITY AND SPECIFICITY;

NONCODING RNA DETECTION; REAL-TIME PCR; STEM-LOOP PRIMER; TAQMAN PCR;

DNA PRIMERS; DNA PROBES; ESCHERICHIA COLI; FLUORESCENT DYES; GENE EXPRESSION; GENE EXPRESSION PROFILING; HEK293 CELLS; HUMANS; INVERTED REPEAT SEQUENCES; LISTERIA MONOCYTOGENES; MICRORNAS; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, BACTERIAL; RNA, SMALL UNTRANSLATED; SENSITIVITY AND SPECIFICITY;

EID: 84888420013     PISSN: 13558382     EISSN: 14699001     Source Type: Journal    
DOI: 10.1261/rna.040501.113     Document Type: Article

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