Whole-mount immunostaining of Drosophila skeletal muscle

Nature Protocols

Volumn 8, Issue 12, 2013, Pages 2496-2501

  Hunt, Liam C ^a   Demontis, Fabio ^a  

^a ST JUDE CHILDREN S RESEARCH HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY;

ADIPOSE TISSUE; AGING; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BRAIN; CONTROLLED STUDY; DEHYDRATION; DROSOPHILA MELANOGASTER; FEMALE; FREEZING; IMMUNOHISTOCHEMISTRY; MALE; MUSCLE DEVELOPMENT; MUSCLE GROWTH; NONHUMAN; PRIORITY JOURNAL; RETINA; SKELETAL MUSCLE; TISSUE STRUCTURE; ULTRASTRUCTURE;

DROSOPHILA MELANOGASTER;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; DROSOPHILA MELANOGASTER; GENOTYPE; GREEN FLUORESCENT PROTEINS; IMMUNOHISTOCHEMISTRY; MUSCLE, SKELETAL; RECOMBINANT PROTEINS;

EID: 84888356912     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2013.156     Document Type: Article

References (35)

1. Nair, K.S. Aging muscle. Am. J. Clin. Nutr. 81, 953-963 (2005).
2. Demontis, F., Piccirillo, R., Goldberg, A.L. & Perrimon, N. Mechanisms of skeletal muscle aging: insights from Drosophila and mammalian models. Dis. Model Mech. doi:10.1242/dmm.012559 (2013).
3. Demontis, F., Piccirillo, R., Goldberg, A.L. & Perrimon, N. The influence of skeletal muscle on systemic aging and lifespan. Aging Cell doi:10.1111/acel.12126 (2013).
4. Miller, M.S. et al. Aging enhances indirect flight muscle fiber performance yet decreases flight ability in Drosophila. Biophys. J. 95, 2391-2401 (2008).
5. Rhodenizer, D., Martin, I., Bhandari, P., Pletcher, S.D. & Grotewiel, M. Genetic and environmental factors impact age-related impairment of negative geotaxis in Drosophila by altering age-dependent climbing speed. Exp. Gerontol. 43, 739-748 (2008).
6. Kenyon, C.J. The genetics of ageing. Nature 464, 504-512 (2010).
7. Bate, M. The embryonic development of larval muscles in Drosophila. Development 110, 791-804 (1990).
8. Demontis, F. & Perrimon, N. Integration of insulin receptor/Foxo signaling and dMyc activity during muscle growth regulates body size in Drosophila. Development 136, 983-993 (2009).
9. Abmayr, S.M., Erickson, M.S. & Bour, B.A. Embryonic development of the larval body wall musculature of Drosophila melanogaster. Trends Genet. 11, 153-159 (1995).
10. Morriss, G.R. et al. Analysis of skeletal muscle development in Drosophila. Methods Mol. Biol. 798, 127-152 (2012).
11. Dutta, D., Anant, S., Ruiz-Gomez, M., Bate, M. & Vijay Raghavan, K. Founder myoblasts and fibre number during adult myogenesis in Drosophila. Development 131, 3761-3772 (2004).
12. Metzger, T. et al. MAP and kinesin-dependent nuclear positioning is required for skeletal muscle function. Nature 484, 120-124 (2012).
13. Cripps, R.M., Lovato, T.L. & Olson, E.N. Positive autoregulation of the myocyte enhancer factor-2 myogenic control gene during somatic muscle development in Drosophila. Dev. Biol. 267, 536-547 (2004).
14. Gilsohn, E. & Volk, T. A screen for tendon-specific genes uncovers new and old components involved in muscle-tendon interaction. Fly 4, 149-153 (2010).
15. Maqbool, T. & Jagla, K. Genetic control of muscle development: learning from Drosophila. J. Muscle Res. Cell Motil. 28, 397-407 (2007).
16. Schonbauer, C. et al. Spalt mediates an evolutionarily conserved switch to fibrillar muscle fate in insects. Nature 479, 406-409 (2011).
17. Piccirillo, R., Demontis, F., Perrimon, N. & Goldberg, A.L. Mechanisms of muscle growth and atrophy in mammals and Drosophila. Dev. Dyn. doi:10.1002/dvdy.24036 (2013).
18. Kucherenko, M.M. et al. Paraffin-embedded and frozen sections of Drosophila adult muscles. J. Vis. Exp. 46, e2438 (2010).
19. Llamusi, B. et al. Muscleblind, BSF and TBPH are mislocalized in the muscle sarcomere of a Drosophila myotonic dystrophy model. Dis. Model Mech. 6, 184-196 (2013).
20. Mukherjee, P., Gildor, B., Shilo, B.Z., Vijay Raghavan, K. & Schejter, E.D. The actin nucleator WASp is required for myoblast fusion during adult Drosophila myogenesis. Development 138, 2347-2357 (2011).
21. Pantoja, M., Fischer, K.A., Ieronimakis, N., Reyes, M. & Ruohola-Baker, H. Genetic elevation of sphingosine 1-phosphate suppresses dystrophic muscle phenotypes in Drosophila. Development 140, 136-146 (2013).
22. Demontis, F. & Dahmann, C. Characterization of the Drosophila ortholog of the human Usher Syndrome type 1G protein sans. PLoS ONE 4, e4753 (2009).
23. Perkins, A.D. et al. Integrin-mediated adhesion maintains sarcomeric integrity. Dev. Biol. 338, 15-27 (2010).
24. Pitsouli, C. & Perrimon, N. The homeobox transcription factor cut coordinates patterning and growth during Drosophila airway remodeling. Sci. Signal. 6, ra12 (2013).
25. Schlichting, K., Wilsch-Brauninger, M., Demontis, F. & Dahmann, C. Cadherin Cad99C is required for normal microvilli morphology in Drosophila follicle cells. J. Cell Sci. 119, 1184-1195 (2006).
26. Demontis, F. & Perrimon, N. FOXO/4E-BP signaling in Drosophila muscles regulates organism-wide proteostasis during aging. Cell 143, 813-825 (2010).
27. Brandt, A., Krohne, G. & Grosshans, J. The farnesylated nuclear proteins KUGELKERN and LAMIN B promote aging-like phenotypes in Drosophila flies. Aging Cell 7, 541-551 (2008).
28. Schuster, C.M., Davis, G.W., Fetter, R.D. & Goodman, C.S. Genetic dissection of structural and functional components of synaptic plasticity. I. Fasciclin II controls synaptic stabilization and growth. Neuron 17, 641-654 (1996).
29. Gajewski, K.M. & Schulz, R.A. CF2 represses Actin 88F gene expression and maintains filament balance during indirect flight muscle development in Drosophila. PLoS ONE 5, e10713 (2010).
30. Budnik, V. et al. Regulation of synapse structure and function by the Drosophila tumor suppressor gene dlg. Neuron 17, 627-640 (1996).
31. Bryantsev, A.L., Baker, P.W., Lovato, T.L., Jaramillo, M.S. & Cripps, R.M. Differential requirements for myocyte enhancer factor-2 during adult myogenesis in Drosophila. Dev. Biol. 361, 191-207 (2012).
32. Bai, J., Hartwig, J.H. & Perrimon, N. SALS, a WH2-domain-containing protein, promotes sarcomeric actin filament elongation from pointed ends during Drosophila muscle growth. Dev. Cell 13, 828-842 (2007).
33. Clyne, P.J., Brotman, J.S., Sweeney, S.T. & Davis, G. Green fluorescent protein tagging Drosophila proteins at their native genomic loci with small P elements. Genetics 165, 1433-1441 (2003).
34. Buszczak, M. et al. The Carnegie protein trap library: a versatile tool for Drosophila developmental studies. Genetics 175, 1505-1531 (2007).
35. Quinones-Coello, A.T. et al. Exploring strategies for protein trapping in Drosophila. Genetics 175, 1089-1104 (2007).