High-content screening and analysis of the golgi complex

Methods in Cell Biology

Volumn 118, Issue , 2013, Pages 281-295

  Galea, George ^a   Simpson, Jeremy C ^a  

^a UNIVERSITY COLLEGE DUBLIN   (Ireland)

Author keywords

Golgi structure; High content analysis; High content screening; RNAi screen

Indexed keywords

SMALL INTERFERING RNA;

ARTICLE; FLUORESCENCE MICROSCOPY; GENE SILENCING; GENETIC TRANSFECTION; GENETICS; GOLGI COMPLEX; GOLGI STRUCTURE; HELA CELL LINE; HIGH THROUGHPUT SCREENING; HIGH-CONTENT ANALYSIS; HIGH-CONTENT SCREENING; HUMAN; METABOLISM; METHODOLOGY; RNA INTERFERENCE; RNAI SCREEN; ULTRASTRUCTURE;

GOLGI STRUCTURE; HIGH-CONTENT ANALYSIS; HIGH-CONTENT SCREENING; RNAI SCREEN;

GENE KNOCKDOWN TECHNIQUES; GOLGI APPARATUS; HELA CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MICROSCOPY, FLUORESCENCE; RNA INTERFERENCE; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 84888209357     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-417164-0.00017-3     Document Type: Chapter

References (26)

1. Au C.E., Bell A.W., Gilchrist A., Hiding J., Nilsson T., Bergeron J.J. Organellar proteomics to create the cell map. Current Opinion in Cell Biology 2007, 19:376-385.
2. Bard F., Casano L., Mallabiabarrena A., Wallace E., Saito K., Kitayama H., et al. Functional genomics reveals genes involved in protein secretion and Golgi organization. Nature 2006, 439:604-607.
3. Boland M.V., Murphy R.F. A neural network classifier capable of recognizing the patterns of all major subcellular structures in fluorescence microscope images of HeLa cells. Bioinformatics 2001, 17:1213-1223.
4. Carpenter A.E., Jones T.R., Lamprecht M.R., Clarke C., Kang I.H., Friman O., et al. Cell Profiler: Image analysis software for identifying and quantifying cell phenotypes. Genome Biology 2006, 7(10):R100.
5. Chen S.C., Murphy R.F. A graphical model approach to automated classification of protein subcellular location patterns in multi-cell images. BMC Bioinformatics 2006, 7:90.
6. Chia J., Goh G., Racine V., Ng S., Kumar P., Bard F. RNAi screening reveals a large signaling network controlling the Golgi apparatus in human cells. Molecular Systems Biology 2012, 8:629.
7. Collinet C., Stöter M., Bradshaw C.R., Samusik N., Rink J.C., Kenski D., et al. Systems survey of endocytosis by multiparametric image analysis. Nature 2010, 464:243-249.
8. Conrad C., Erfle H., Warnat P., Daigle N., Lörch T., Ellenberg J., et al. Automatic identification of subcellular phenotypes on human cell arrays. Genome Research 2004, 14(6):1130-1136.
9. de Forges H., Bouissou A., Perez F. Interplay between microtubule dynamics and intracellular organization. The International Journal of Biochemistry & Cell Biology 2012, 44:266-274.
10. Dejgaard S.Y., Murshid A., Dee K.M., Presley J.F. Confocal microscopy-based linescan methodologies for intra-Golgi localization of proteins. Journal of Histochemistry and Cytochemistry 2007, 55:709-719.
11. Dorval T., Ogier A., Genovesio A., Lim H.K., Kwon do Y., Lee J.H., et al. Contextual automated 3D analysis of subcellular organelles adapted to high-content screening. Journal of Biomolecular Screening 2010, 15:847-857.
12. Eliceiri K.W., Berthold M.R., Goldberg I.G., Ibanez L., Manjunath B.S., Martone M.E., et al. Biological imaging software tools. Nature Methods 2012, 9:697-710.
13. Erfle H., Neumann B., Rogers P., Bulkescher J., Ellenberg J., Pepperkok R. Work flow for multiplexing siRNA assays by solid-phase reverse transfection in multiwell plates. Journal of Biomolecular Screening 2008, 13:575-580.
14. Giuliano K.A., DeBiasio R.L., Dunlay R.T., Gough A., Volosky J.M., Zock J., et al. High-content screening: A new approach to easing key bottlenecks in the drug discovery process. Journal of Biomolecular Screening 1997, 2:249-259.
15. Hamilton N.A., Pantelic R.S., Hanson K., Teasdale R.D. Fast automated cell phenotype image classification. BMC Bioinformatics 2007, 8:110.
16. Liebel U., Starkuviene V., Erfle H., Simpson J.C., Poustka A., Wiemann S., et al. A microscope-based screening platform for large-scale functional protein analysis in intact cells. FEBS Letters 2003, 554:394-398.
17. Neumann B., Walter T., Hériché J.K., Bulkescher J., Erfle H., Conrad C., et al. Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes. Nature 2010, 464:721-727.
18. Niederlein A., Meyenhofer F., White D., Bickle M. Image analysis in high-content screening. Combinatorial Chemistry & High Throughput Screening 2009, 12:899-907.
19. Pereira D.A., Williams J.A. Origin and evolution of high throughput screening. British Journal of Pharmacology 2007, 152:53-61.
20. Ramirez I.B., Lowe M. Golgins and GRASPs: Holding the Golgi together. Seminars in Cell & Developmental Biology 2009, 20:770-779.
21. Shariff A., Kangas J., Coelho L.P., Quinn S., Murphy R.F. Automated image analysis for high-content screening and analysis. Journal of Biomolecular Screening 2010, 15:726-734.
22. Simpson J.C., Joggerst B., Laketa V., Verissimo F., Cetin C., Erfle H., et al. Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathway. Nature Cell Biology 2012, 14:764-774.
23. Starkuviene V., Liebel U., Simpson J.C., Erfle H., Poustka A., Wiemann S., et al. High-content screening microscopy identifies novel proteins with a putative role in secretory membrane traffic. Genome Research 2004, 14(10A):1-11.
24. Storrie B., White J., Röttger S., Stelzer E.H., Suganuma T., Nilsson T. Recycling of golgi-resident glycosyltransferases through the ER reveals a novel pathway and provides an explanation for nocodazole-induced Golgi scattering. The Journal of Cell Biology 1998, 143:1505-1521.
25. Taylor D.L. A personal perspective on high-content screening (HCS): From the beginning. Journal of Biomolecular Screening 2010, 15:720-725.
26. Wendler F., Gillingham A.K., Sinka R., Rosa-Ferreira C., Gordon D.E., Franch-Marro X., et al. A genome-wide RNA interference screen identifies two novel components of the metazoan secretory pathway. EMBO Journal 2010, 29:304-314.