A surface-tethered spheroid model for functional evaluation of 3T3-L1 adipocytes

Biotechnology and Bioengineering

Volumn 111, Issue 1, 2014, Pages 174-183

  Turner, Paul A ^a   Harris, Lacey M ^a   Purser, Christine A ^b   Baker, Rodney C ^b   Janorkar, Amol V ^a  

^a UNIVERSITY OF MISSISSIPPI MEDICAL CENTER   (United States)

^b UNIVERSITY OF MISSISSIPPI SCHOOL OF MEDICINE   (United States)

Author keywords

3D culture model; Adipocytes; Surface tethered

Indexed keywords

3-D CULTURES; ADIPOCYTES; ELASTIN-LIKE POLYPEPTIDES; GENETICALLY ENGINEERED POLYMERS; PREADIPOCYTE DIFFERENTIATION; STATISTICAL DIFFERENCES; SURFACE-TETHERED; TISSUE CULTURE POLYSTYRENES;

ENZYME INHIBITION; FATTY ACIDS; MOLECULAR WEIGHT; MONOLAYERS; TISSUE; TISSUE CULTURE;

THREE DIMENSIONAL;

DNA; ELASTIN; FATTY ACID; POLYETHYLENEIMINE; POLYSTYRENE; PROTEIN; TRIACYLGLYCEROL; AZO COMPOUND; OIL RED O;

ADIPOCYTE; ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; BIOACCUMULATION; CELL CULTURE; CELL DIFFERENTIATION; CELL FUNCTION; CELL STRAIN 3T3; CELL VIABILITY; CONTROLLED STUDY; DNA CONTENT; FATTY ACID TRANSPORT; IN VITRO STUDY; MONOLAYER CULTURE; MOUSE; NONHUMAN; PROADIPOCYTE; PROTEIN CONTENT; THREE DIMENSIONAL CELL CULTURE; TISSUE CULTURE; 3T3 CELL LINE; ANALYSIS OF VARIANCE; ANIMAL; BIOLOGICAL MODEL; BIOTECHNOLOGY; CELL CULTURE TECHNIQUE; CHEMISTRY; CYTOLOGY; METABOLISM; MULTICELLULAR SPHEROID; PARTICLE SIZE; PROCEDURES; SURFACE PROPERTY;

3T3-L1 CELLS; ADIPOCYTES; ANALYSIS OF VARIANCE; ANIMALS; AZO COMPOUNDS; BIOTECHNOLOGY; CELL CULTURE TECHNIQUES; DNA; FATTY ACIDS; MICE; MODELS, BIOLOGICAL; PARTICLE SIZE; SPHEROIDS, CELLULAR; SURFACE PROPERTIES; TRIGLYCERIDES;

EID: 84888056292     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25099     Document Type: Article

References (34)

1. Bligh EG, Dyer WJ. 1959. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911-917.
2. Burr GO, Burr MM, Miller E. 1930. On the nature and role of the fatty acids essential in nutrition. J Biol Chem 86:5871-5879.
3. Calder PC. 2006. n-3 polyunsaturated fatty acids, inflammation, and inflammatory diseases. Am J Clin Nutr 83:1505S-1519S.
4. Cheng NC, Wang S, Young TH. 2012. The influence of spheroid formation of human adipose-derived stem cells on chitosan films on stemness and differentiation capabilities. Biomaterials 33:1748-1758.
5. Daya S, Loughlin AJ, MacQueen HA. 2007. Culture and differentiation in two-dimensional and three-dimensional in vitro systems. Differentiation 75:360-370.
6. Duranti F, Salti G, Bovani B, Calandra M, Rosati ML. 1998. Injectable hyaluronic acid gel for soft tissue augmentation. Dermatol Surg 24:1317-1325.
7. Evans M, Geigerman C, Cook J, Curtis L, Kuebler B, McIntosh M. 2000. Conjugated linoleic acid suppresses triglyceride accumulation and induces apoptosis in 3T3-L1 preadipocytes. Lipids 35:899-910.
8. Flegal KM, Carroll MD, Odgen CL, Curtin LR. 2010. Prevalence and trends in obesity among US adults, 1999-2008. JAMA 303:235-241.
9. Gomillion CT, Burg KJL. 2006. Stem cells and adipose tissue engineering. Biomaterials 27:6052-6063.
10. Gottschling-Zeller H, Birgel M, Scriba D, Blum WF, Hauner H. 1999. Depot-specific release of leptin from subcutaneous and omental adipocytes in suspension culture: Effect of tumor necrosis factor-alpha and transforming growth factor-beta1. Eur J Endocrinol 141:436-442.
11. Janorkar AV, Rajagopalan P, Yarmush ML, Megeed Z. 2008. ELP-polyelectrolyte structure confers control over hepatocyte morphology and function. Biomaterials 29:625-632.
12. Kang X, Yubing X, Powell HM, Lee LJ, Belury MA, Lannutti JJ, Kniss DA. 2007. Adipogenesis of murine embryonic stem cells in a three-dimensional culture system using electrospun polymer scaffolds. Biomaterials 28:450-458.
13. Kokatnur MG, Oalmann MC, Johnson WD, Malcom GT, Strong JP. 1979. Fatty acid composition of human adipose tissue from two anatomical sites in a biracial community. Am J Clin Nutr 11:2198-2205.
14. Kokta TA, Strat AL, Papasani MR, Szasz JI, Dodson MV, Hill RA. 2008. Regulation of lipid accumulation in 3T3-L1 cells: Insulin-independent and combined effects of fatty acids and insulin. Animal 2:92-99.
15. Lau DC, Dhillon B, Yan H, Szmitko PE, Verma S. 2005. Adipokines: Molecular links between obesity and atherosclerosis. Am J Physiol Heart Circ Physiol 288:2031-2041.
16. Lopez IP, Marti A, Milagro FI, Zulet Md Mde L, Moreno-Aliaga MJ, Martinez JA, De Miguel C. 2003. DNA microarray analysis of genes differentially expressed in diet-induced (cafeteria) obese rats. Obes Res 11:188-194.
17. Madsen L, Peterson RK, Kristiansen K. 2005. Regulation of adipocyte differentiation and function by polyunsaturated fatty acids. Biochim Biophys Acta 1740:266-286.
18. Marler JJ, Guha A, Rowley J, Koka R, Mooney D, Upton J, Vacanti JP. 2000. Soft-tissue Augmentation with injectable alginate and syngeneic fibroblasts. Plast Reconstr Surg 105:2049-2058.
19. Moldket AH, Serdula MK, Dietz WH, Bowman BA, Marks JS, Koplan JP. 1999. The spread of the obesity epidemic in the United States, 1991-1998. JAMA 282:1519-1522.
20. Mueller-Klieser W. 1997. Three-dimensional cell cultures: From molecular mechanisms to clinical applications. Am J Physiol 273:1109-1123.
21. Patel PN, Gobin AS, West JL, Patrick CW, Jr. 2005. Poly(ethylene glycol) hydrogel system supports preadipocyte viability, adhesion, and proliferation. Tissue Eng 11:1498-1505.
22. Patrick CW, Jr. 2000. Adipose tissue engineering: The future of breast and soft tissue reconstruction following tumor resection. Semin Surg Oncol 19(3):302-311.
23. Patrick CW, Jr. 2001. Tissue engineering strategies for adipose tissue repair. Anat Rec 263:361-366.
24. Pi-Sunyer FX. 2002. The obesity epidemic: Pathophysiology and consequences of obesity. Obes Res 10:97S-103S.
25. Rosen OM, Smith CJ, Hirsch A, Lai E, Rubin CS. 1979. Recent studies of the 3T3-L1 adipocyte-like cell line. Recent Prog Horm Res 35:477-499.
26. Shiomi N, Maeda M, Mimura M. 2011. Compounds that inhibit triglyceride accumulation and TNFα secretion in adipocytes. J Biomed Sci Eng 4:684-691.
27. Tanzi MC, Fare S. 2009. Adipose tissue engineering: state of the art, recent advances and innovative approaches. Expert Rev Med Devices 6:533-551.
28. Todoric J, Loffler M, Huber J, Bilban M, Reimers M, Kadl A, Zeyda M, Waldhausl W, Stulnig TM. 2006. Adipose tissue inflammation induced by high-fat diet in obese diabetic mice is prevented by n-3 polyunsaturated fatty acids. Diabetologia 49:2109-2119.
29. Turner PA, Weeks CA, McMurphy AJ, Janorkar AV. 2013. Spheroid organization kinetics of H35 rat hepatoma model cell system on elastin-like polypeptide-polyethyleneimine copolymer substrates. J Biomed Mater Res A (available online ahead of print).
30. Wang Y, Beydoun MA, Liang L, Caballero B, Kumanyika SK. 2008. Will all Americans become overweight or obese? Estimating the progression and cost of the US obesity epidemic. Obesity 16:2323-2330.
31. Wang W, Itaka K, Ohba S, Nishiyama N, Chung UI, Yamasaki Y, Kataoka K. 2009. 3D spheroid culture system on micropatterned substrates for improved differentiation efficiency of multipotent mesenchymal stem cells. Biomaterials 30:2705-2715.
32. Weeks CA, Newman K, Turner PA, Rodysill B, Hickney RD, Nyberg SL, Janorkar AV. 2013. Suspension culture of hepatocyte-derived reporter cells in presence of albumin to form stable three-dimensional spheroids. Biotechnol Bioeng 110:2548-2555.
33. Yamada KM, Cukierman E. 2007. Modeling tissue morphogenesis and cancer in 3D. Cell 130:601-610.
34. Zhang HH, Kumer S, Barnett AH, Eggo MC. 2000. Ceiling culture of mature human adipocytes: Use in studies of adipocyte functions. J Endocrinol 164:119-128.