The influence of electrospun scaffold topography on endothelial cell morphology, alignment, and adhesion in response to fluid flow

Biotechnology and Bioengineering

Volumn 111, Issue 1, 2014, Pages 184-195

  Whited, Bryce M ^a   Rylander, Marissa Nichole ^a,b  

^a VIRGINIA POLYTECHNIC INSTITUTE AND STATE UNIVERSITY   (United States)

^b State University   (United States)

Author keywords

Electrospinning; Endothelial cell; Fluid flow; Scaffold; Vascular tissue engineering

Indexed keywords

CELL MORPHOLOGY; CELL-CELL JUNCTIONS; COMPOSITE POLYMER; CONFLUENT MONOLAYERS; DEGREE OF ALIGNMENTS; ELECTROSPUN SCAFFOLDS; STRUCTURAL PROTEINS; VASCULAR TISSUE ENGINEERING;

ADHESION; ALIGNMENT; CELL MEMBRANES; CYTOLOGY; ELECTROSPINNING; ENDOTHELIAL CELLS; FIBERS; FLOW OF FLUIDS; PROTEINS; SCAFFOLDS; TOPOGRAPHY;

SCAFFOLDS (BIOLOGY);

COLLAGEN TYPE 1; F ACTIN; VASCULAR ENDOTHELIAL CADHERIN; VINCULIN;

ARTICLE; CELL ADHESION; CELL JUNCTION; CELL STRUCTURE; ELECTROSPINNING; ENDOTHELIUM; ENDOTHELIUM CELL; FLUID FLOW; HUMAN; IMAGE ANALYSIS; IMMUNOFLUORESCENCE; PROTEIN EXPRESSION; TISSUE ENGINEERING; TOPOGRAPHY; VASCULAR TISSUE; CELL SHAPE; CHEMISTRY; CYTOLOGY; ELECTROCHEMICAL ANALYSIS; PHYSIOLOGY; TISSUE SCAFFOLD; UMBILICAL VEIN ENDOTHELIAL CELL;

CELL ADHESION; CELL SHAPE; ELECTROCHEMICAL TECHNIQUES; ENDOTHELIAL CELLS; HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS; HUMANS; TISSUE SCAFFOLDS;

EID: 84888049650     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24995     Document Type: Article

References (44)

1. Aird WC. 2007. Phenotypic heterogeneity of the endothelium: I. Structure, function, and mechanisms. Circ Res 100:158-173.
2. Aird WC. 2007. Phenotypic heterogeneity of the endothelium: II. Representative vascular beds. Circ Res 100:174-190.
3. Baguneid M, Murray D, Salacinski HJ, Fuller B, Hamilton G, Walker M, Seifalian AM. 2004. Shear-stress preconditioning and tissue-engineering-based paradigms for generating arterial substitutes, Biotechnol Appl Biochem 39:151-157.
4. Barbee KA, Davies PF, Lal R. 1994. Shear stress-induced reorganization of the surface topography of living endothelial cells imaged by atomic force microscopy, Circ Res 74:163-171.
5. Bashur CA, Dahlgren LA, Goldstein AS. 2006. Effect of fiber diameter and orientation on fibroblast morphology and proliferation on electrospun poly(d, l-lactic-co-glycolic acid) meshes, Biomaterials 27:5681-5688.
6. Cameron A, Davis KB, Green G, Schaff HV. 1996. Coronary bypass surgery with internal-thoracic-artery grafts-Effects on survival over a 15-year period, N Engl J Med 334:216-219.
7. Choi JS, Lee SJ, Christ GJ, Atala A, Yoo JJ. 2008. The influence of electrospun aligned poly(epsilon-caprolactone)/collagen nanofiber meshes on the formation of self-aligned skeletal muscle myotubes, Biomaterials 29:2899-2906.
8. Cines DB, Pollak ES, Buck CA, Loscalzo J, Zimmerman GA, McEver RP, Pober JS, Wick TM, Konkle BA, Schwartz BS, Barnathan ES, McCrae KR, Hug BA, Schmidt AM, Stern DM. 1998. Endothelial cells in physiology and in the pathophysiology of vascular disorders, Blood 91:3527-3561.
9. Conte MS. 1998. The ideal small arterial substitute: A search for the Holy Grail? FASEB J 12:43-45.
10. Dardik A, Liu A, Ballermann BJ. 1999. Chronic in vitro shear stress stimulates endothelial cell retention on prosthetic vascular grafts and reduces subsequent in vivo neointimal thickness, J Vasc Surg 29:157-167.
11. Davies PF. 1995. Flow-mediated endothelial mechanotransduction, Physiol Rev 75:519-560.
12. Flemming RG, Murphy CJ, Abrams GA, Goodman SL, Nealey PF. 1999. Effects of synthetic micro- and nano-structured surfaces on cell behavior, Biomaterials 20:573-588.
13. Grenier G, Remy-Zolghadri M, Guignard R, Bergeron F, Labbe R, Auger FA, Germain L. 2003. Isolation and culture of the three vascular cell types from a small vein biopsy sample, In Vitro Cell Dev Biol Anim 39:131-139.
14. Heath DE, Lannutti JJ, Cooper SL. 2010. Electrospun scaffold topography affects endothelial cell proliferation, metabolic activity, and morphology, J Biomed Mater Res A 94:1195-1204.
15. Humphries JD, Wang P, Streuli C, Geiger B, Humphries MJ, Ballestrem C. 2007. Vinculin controls focal adhesion formation by direct interactions with talin and actin, J Cell Biol 179:1043-1057.
16. Inoguchi H, Tanaka T, Maehara Y, Matsuda T. 2007. The effect of gradually graded shear stress on the morphological integrity of a huvec-seeded compliant small-diameter vascular graft, Biomaterials 28:486-495.
17. Jiang XY, Takayama S, Qian XP, Ostuni E, Wu HK, Bowden N, LeDuc P, Ingber DE, Whitesides GM. 2002. Controlling mammalian cell spreading and cytoskeletal arrangement with conveniently fabricated continuous wavy features on poly(dimethylsiloxane), Langmuir 18:3273-3280.
18. Ju YM, Choi JS, Atala A, Yoo JJ, Lee SJ. 2010. Bilayered scaffold for engineering cellularized blood vessels, Biomaterials 31:4313-4321.
19. Kaushal S, Amiel GE, Guleserian KJ, Shapira OM, Perry T, Sutherland FW, Rabkin E, Moran AM, Schoen FJ, Atala A, Soker S, Bischoff J, Mayer JE, Jr. 2001. Functional small-diameter neovessels created using endothelial progenitor cells expanded ex vivo, Nat Med 7:1035-1040.
20. Kreke MR, Sharp LA, Lee YW, Goldstein AS. 2008. Effect of intermittent shear stress on mechanotransductive signaling and osteoblastic differentiation of bone marrow stromal cells, Tissue Eng Part A 14:529-537.
21. Langille BL, Graham JJ, Kim D, Gotlieb AI. 1991. Dynamics of shear-induced redistribution of F-actin in endothelial cells in vivo, Arterioscler Thromb 11:1814-1820.
22. Lee SJ, Yoo JJ, Lim GJ, Atala A, Stitzel J. 2007. In vitro evaluation of electrospun nanofiber scaffolds for vascular graft application, J Biomed Mater Res A 83:999-1008.
23. Lee SJ, Liu J, Oh SH, Soker S, Atala A, Yoo JJ. 2008. Development of a composite vascular scaffolding system that withstands physiological vascular conditions, Biomaterials 29:2891-2898.
24. L'Heureux N, Dusserre N, Konig G, Victor B, Keire P, Wight TN, Chronos NA, Kyles AE, Gregory CR, Hoyt G, Robbins RC, McAllister TN. 2006. Human tissue-engineered blood vessels for adult arterial revascularization, Nat Med 12:361-365.
25. L'Heureux N, McAllister TN, de la Fuente LM. 2007. Tissue-engineered blood vessel for adult arterial revascularization. N Engl J Med 357:1451-1453.
26. Liliensiek SJ, Wood JA, Yong J, Auerbach R, Nealey PF, Murphy CJ. 2010. Modulation of human vascular endothelial cell behaviors by nanotopographic cues, Biomaterials 31:5418-5426.
27. Lim SH, Liu XY, Song H, Yarema KJ, Mao HQ. 2010. The effect of nanofiber-guided cell alignment on the preferential differentiation of neural stem cells, Biomaterials 31:9031-9039.
28. Mott RE, Helmke BP. 2007. Mapping the dynamics of shear stress-induced structural changes in endothelial cells, Am J Physiol Cell Physiol 293:C1616-C1626.
29. Nieponice A, Soletti L, Guan J, Deasy BM, Huard J, Wagner WR, Vorp DA. 2008. Development of a tissue-engineered vascular graft combining a biodegradable scaffold, muscle-derived stem cells and a rotational vacuum seeding technique, Biomaterials 29:825-833.
30. Niklason LE, Gao J, Abbott WM, Hirschi KK, Houser S, Marini R, Langer R. 1999. Functional arteries grown in vitro, Science 284:489-493.
31. Pham QP, Sharma U, Mikos AG. 2006. Electrospun poly(epsilon-caprolactone) microfiber and multilayer nanofiber/microfiber scaffolds: Characterization of scaffolds and measurement of cellular infiltration. Biomacromolecules 7:2796-2805.
32. Quint C, Kondo Y, Manson RJ, Lawson JH, Dardik A, Niklason LE. 2011. Decellularized tissue-engineered blood vessel as an arterial conduit, Proc Natl Acad Sci USA 108:9214-9219.
33. Seifalian AM, Tiwari A, Hamilton G, Salacinski HJ. 2002. Improving the clinical patency of prosthetic vascular and coronary bypass grafts: The role of seeding and tissue engineering. Artif Organs 26:307-320.
34. Shireman PK, Pearce WH. 1996. Endothelial cell function: Biologic and physiologic functions in health and disease, Am J Roentgenol 166:7-13.
35. Sieminski AL, Hebbel RP, Gooch KJ. 2005. Improved microvascular network in vitro by human blood outgrowth endothelial cells relative to vessel-derived endothelial cells, Tissue Eng 11:1332-1345.
36. Silver AE, Vita JA. 2006. Shear-stress-mediated arterial remodeling in atherosclerosis: Too much of a good thing? Circulation 113:2787-2789.
37. Tillman BW, Yazdani SK, Lee SJ, Geary RL, Atala A, Yoo JJ. 2009. The in vivo stability of electrospun polycaprolactone-collagen scaffolds in vascular reconstruction, Biomaterials 30:583-588.
38. Uttayarat P, Toworfe GK, Dietrich F, Lelkes PI, Composto RJ. 2005. Topographic guidance of endothelial cells on silicone surfaces with micro- to nanogrooves: Orientation of actin filaments and focal adhesions. J Biomed Mater Res A 75:668-680.
39. Uttayarat P, Perets A, Li M, Pimton P, Stachelek SJ, Alferiev I, Composto RJ, Levy RJ, Lelkes PI. 2010. Micropatterning of three-dimensional electrospun polyurethane vascular grafts, Acta Biomater 6:4229-4237.
40. Veleva AN, Heath DE, Johnson JK, Nam J, Patterson C, Lannutti JJ, Cooper SL. 2009. Interactions between endothelial cells and electrospun methacrylic terpolymer fibers for engineered vascular replacements, J Biomed Mater Res A 91:1131-1139.
41. Williams C, Wick TM. 2004. Perfusion bioreactor for small diameter tissue-engineered arteries, Tissue Eng 10:930-941.
42. Williamson MR, Black R, Kielty C. 2006. PCL-PU composite vascular scaffold production for vascular tissue engineering: Attachment, proliferation and bioactivity of human vascular endothelial cells. Biomaterials 27:3608-3616.
43. Yazdani SK, Tillman BW, Berry JL, Soker S, Geary RL. 2010. The fate of an endothelium layer after preconditioning, J Vasc Surg 51:174-183.
44. Yin Z, Chen X, Chen JL, Shen WL, Hieu Nguyen TM, Gao L, Ouyang HW. 2010. The regulation of tendon stem cell differentiation by the alignment of nanofibers, Biomaterials 31:2163-2175.