Direct measurement of intranuclear strain distributions and rna synthesis in single cells embedded within native tissue

Biophysical Journal

Volumn 105, Issue 10, 2013, Pages 2252-2261

  Henderson, Jonathan T ^a   Shannon, Garrett ^a   Veress, Alexander I ^b   Neu, Corey P ^a  

^a PURDUE UNIVERSITY   (United States)

^b University of Washington   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RNA;

ANIMAL; ARTICLE; ARTICULAR CARTILAGE; BIOSYNTHESIS; CATTLE; CELL NUCLEUS; CHROMATIN; CYTOLOGY; EXTRACELLULAR MATRIX; GENETICS; MECHANICAL STRESS; METABOLISM; PHYSIOLOGY; SINGLE CELL ANALYSIS; TUMOR MICROENVIRONMENT; WEIGHT BEARING;

ANIMALS; CARTILAGE, ARTICULAR; CATTLE; CELL NUCLEUS; CELLULAR MICROENVIRONMENT; CHROMATIN; EXTRACELLULAR MATRIX; RNA; SINGLE-CELL ANALYSIS; STRESS, MECHANICAL; WEIGHT-BEARING;

EID: 84887964162     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1016/j.bpj.2013.09.054     Document Type: Article

References (26)

1. T. Misteli Protein dynamics: implications for nuclear architecture and gene expression Science 291 2001 843 847
2. T. Cremer, and M. Cremer S. Fakan Chromosome territories - a functional nuclear landscape Curr. Opin. Cell Biol. 18 2006 307 316
3. G. Felsenfeld Chromatin unfolds Cell 86 1996 13 19
4. K.J. Polach, and J. Widom A model for the cooperative binding of eukaryotic regulatory proteins to nucleosomal target sites J. Mol. Biol. 258 1996 800 812
5. B.R. Cairns Chromatin remodeling: insights and intrigue from single-molecule studies Nat. Struct. Mol. Biol. 14 2007 989 996
6. N. Wang, J.P. Butler, and D.E. Ingber Mechanotransduction across the cell surface and through the cytoskeleton Science 260 1993 1124 1127
7. N. Wang, J.D. Tytell, and D.E. Ingber Mechanotransduction at a distance: mechanically coupling the extracellular matrix with the nucleus Nat. Rev. Mol. Cell Biol. 10 2009 75 82
8. A. Mammoto, T. Mammoto, and D.E. Ingber Mechanosensitive mechanisms in transcriptional regulation J. Cell Sci. 125 2012 3061 3073
9. M.C. Cardoso, and K. Schneider H. Leonhardt Structure, function and dynamics of nuclear subcompartments Curr. Opin. Cell Biol. 24 2012 79 85
10. F. Guilak Compression-induced changes in the shape and volume of the chondrocyte nucleus J. Biomech. 28 1995 1529 1541
11. M.M. Knight, and J. van de Breevaart Bravenboer D.L. Bader Cell and nucleus deformation in compressed chondrocyte-alginate constructs: temporal changes and calculation of cell modulus Biochim. Biophys. Acta 1570 2002 1 8
12. W.R. Legant, and J.S. Miller C.S. Chen Measurement of mechanical tractions exerted by cells in three-dimensional matrices Nat. Methods 7 2010 969 971
13. K.V. Iyer, and S. Pulford G.V. Shivashankar Mechanical activation of cells induces chromatin remodeling preceding MKL nuclear transport Biophys. J. 103 2012 1416 1428
14. Ouyang, M., J. Sun,., Y. Wang. 2008. Determination of hierarchical relationship of Src and Rac at subcellular locations with FRET biosensors. Proceedings of the National Academy of Sciences of the United States of America. 105:14353-14358.
15. D.D. Chan, and C.P. Neu Transient and microscale deformations and strains measured under exogenous loading by noninvasive magnetic resonance PLoS ONE 7 2012 e33463
16. C.P. Neu, and A.H. Reddi P.E. Di Cesare Increased friction coefficient and superficial zone protein expression in patients with advanced osteoarthritis Arthritis Rheum. 62 2010 2680 2687
17. N.S. Phatak, and Q. Sun J.A. Weiss Noninvasive determination of ligament strain with deformable image registration Ann. Biomed. Eng. 35 2007 1175 1187
18. A. Veress, N. Phatak, and J. Weiss Deformable image registration with hyperelastic warping J. Suri, D. Willson, S. Laxminarayan, Handbook of Biomedical Image Analysis Volume III: Registration Models 2005 Kluwer Academic/Plenum Publishers New York, Boston, Dordrecht, London, Moscow 487 533
19. A.I. Veress, and J.A. Weiss R.D. Rabbitt Strain measurement in coronary arteries using intravascular ultrasound and deformable images J. Biomech. Eng. 124 2002 734 741
20. A.I. Veress, and J.A. Weiss G.T. Gullberg Measuring regional changes in the diastolic deformation of the left ventricle of SHR rats using microPET technology and hyperelastic warping Ann. Biomed. Eng. 36 2008 1104 1117
21. Jao, C. Y., and A. Salic. 2008. Exploring RNA transcription and turnover in vivo by using click chemistry. Proceedings of the National Academy of Sciences of the United States of America. 105:15779-15784.
22. E. Paredes, and S.R. Das Click chemistry for rapid labeling and ligation of RNA ChemBioChem 12 2011 125 131
23. C.L. Gilchrist, and S.W. Witvoet-Braam L.A. Setton Measurement of intracellular strain on deformable substrates with texture correlation J. Biomech. 40 2007 786 794
24. M. Kruithof, and F.T. Chien J. van Noort Single-molecule force spectroscopy reveals a highly compliant helical folding for the 30-nm chromatin fiber Nat. Struct. Mol. Biol. 16 2009 534 540
25. E. Kim, F. Guilak, and M.A. Haider The dynamic mechanical environment of the chondrocyte: a biphasic finite element model of cell-matrix interactions under cyclic compressive loading J. Biomech. Eng. 130 2008 061009
26. C.P. Neu, M.L. Hull, and J.H. Walton Heterogeneous three-dimensional strain fields during unconfined cyclic compression in bovine articular cartilage explants J. Orthop. Res. 23 2005 1390 1398