An Amplex Red-based fluorometric and spectrophotometric assay for l-asparaginase using its natural substrate

Analytical Biochemistry

Volumn 445, Issue 1, 2014, Pages 20-23

  Karamitros, Christos S ^b   Lim, Jiseok ^a,b   Konrad, Manfred ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

^b MAX PLANCK INSTITUTE FOR DYNAMICS AND SELF ORGANIZATION   (Germany)

Author keywords

Amplex Red; Coupled enzyme assay; l Asparaginase; l Aspartate oxidase; Leukemia

Indexed keywords

AMINO ACIDS; AMMONIA;

AMPLEX RED; ASPARTATES; ENZYME ASSAYS; L -ASPARAGINASE; LEUKEMIA;

FLUORESCENCE;

AMMONIA; AMPLEX RED REAGENT; ASPARAGINASE; ASPARTATE OXIDASE; ASPARTIC ACID; HORSERADISH PEROXIDASE; HYDROGEN PEROXIDE; IMINOASPARATE; REAGENT; RESORUFIN; UNCLASSIFIED DRUG;

ARTICLE; ENZYME ACTIVITY; FLUORESCENCE; FLUOROMETRY; HYDROLYSIS; PRIORITY JOURNAL; SPECTROPHOTOMETRY;

ARMORACIA RUSTICANA;

AMPLEX RED; COUPLED ENZYME ASSAY; L-ASPARAGINASE; L-ASPARTATE OXIDASE; LEUKEMIA;

ASPARAGINASE; ENZYME ASSAYS; FLUOROMETRY; HUMANS; OXAZINES; OXIDATION-REDUCTION; SPECTROMETRY, FLUORESCENCE; SUBSTRATE SPECIFICITY;

EID: 84887692019     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.09.028     Document Type: Article

References (21)

1. K. Aghaiypour, A. Wlodawer, and J. Lubkowski Structural basis for the activity and substrate specificity of Erwinia chrysanthemi l-asparaginase Biochemistry 40 2001 5655 5664 (Pubitemid 32440869)
2. M. Kozak, D. Borek, R. Janowski, and M. Jaskolski Crystallization and preliminary crystallographic studies of five crystal forms of Escherichia coli l-asparaginase II (Asp90Glu mutant) Acta Crystallogr. D 58 2002 130 132 (Pubitemid 34099064)
3. A.L. Stecher, P.M. de Deus, I. Polikarpov, and J. Abrahao-Neto Stability of l-asparaginase: an enzyme used in leukemia treatment Pharm. Acta Helv. 74 1999 1 9 (Pubitemid 30035397)
4. M. Duval, S. Suciu, A. Ferster, X. Rialland, B. Nelken, P. Lutz, Y. Benoit, A. Robert, A.M. Manel, E. Vilmer, J. Otten, and N. Philippe Comparison of Escherichia coli-asparaginase with Erwinia-asparaginase in the treatment of childhood lymphoid malignancies: results of a randomized european organization for research and treatment of cancer-children's leukemia group phase 3 trial Blood 99 2002 2734 2739 (Pubitemid 34525359)
5. H.A. Campbell, L.T. Mashburn, E.A. Boyse, and L.J. Old Two l-asparaginases from Escherichia coli B: their separation, purification, and antitumor activity Biochemistry 6 1967 721 730
6. D. Cooney, and R. Handschumacher l-Asparaginase and l-asparagine metabolism Annu. Rev. Pharmacol. 10 1970 421 440
7. Y.Z. Frohwein, M. Friedman, J. Reizer, and N. Grossowicz Sensitive and rapid assay for l-asparaginase Nat. New Biol. 230 1971 158 159
8. C. Lanvers, J.P.V. Pinheiro, G. Hempel, G. Wuerthwein, and J. Boos Analytical validation of a microplate reader-based method for the therapeutic drug monitoring of l-asparaginase in human serum Anal. Biochem. 309 2002 117 126
9. V.M. Balcão, C. Mateo, R. Fernandez-Lafuente, F.X. Malcata, and J.M. Guisa Structural and functional stabilization of l-asparaginase via multisubunit immobilization onto highly activated supports Biotechnol. Prog. 17 2001 537 542 (Pubitemid 32532783)
10. +-dependent asparaginase, NSE1, is crucial for plant growth and seed production in Lotus japonicus Plant Cell Physiol. 54 2013 107 118
11. I.T. Mononen, V.M. Kaartinen, and J.C. Williams A fluorometric assay for glycosylasparaginase activity and detection of aspartylglycosaminuria Anal. Biochem. 208 1993 372 374 (Pubitemid 23084688)
12. P. Ylikangas, and I. Mononen A fluorometric assay for l-asparaginase activity and monitoring of l-asparaginase therapy Anal. Biochem. 280 2000 42 45 (Pubitemid 30210810)
13. J.C. Baret, O.J. Miller, V. Taly, M. Ryckelynck, A. El-Harrak, L. Frenz, C. Rick, M.L. Samuels, J.B. Hutchison, J.J. Agresti, D.R. Link, D.A. Weitzc, and A.D. Griffiths Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity Lab Chip 9 2009 1850 1858
14. J. Lim, P. Gruner, M. Konrad, and J.C. Baret Micro-optical lens array for fluorescence detection in droplet-based microfluidics Lab Chip 13 2013 1472 1475
15. R. Flachmann, N. Kunz, J. Seifert, M. Gutlich, F.J. Wientjes, A. Laufer, and H.G. Gassen Molecular biology of pyridine nucleotide biosynthesis in Escherichia coli Eur. J. Biochem. 175 1988 221 228
16. D.T. Bonthron l-Asparaginase II of Escherichia coli K-12: cloning, mapping, and sequencing of the ansB gene Gene 91 1990 101 105 (Pubitemid 20293241)
17. G. Tedeschi, S. Ronchi, T. Simonic, C. Treu, A. Mattevi, and A. Negri Probing the active site of l-aspartate oxidase by site-directed mutagenesis: role of basic residues in fumarate reduction Biochemistry 40 2001 4738 4744 (Pubitemid 32374661)
18. B.D. Howes, N.C. Brissett, W.A. Doyle, A.T. Smith, and G. Smulevich Spectroscopic and kinetic properties of the horseradish peroxidase mutant T171S: evidence for selective effects on the reduced state of the enzyme FEBS J. 272 2005 5514 5521 (Pubitemid 41579412)
19. 2 release from activated human leukocytes using a dihydroxyphenoxazine derivative J. Immunol. Methods 202 1997 133 141 (Pubitemid 27128755)
20. A. Zhu, R. Romero, and H.R. Petty A sensitive fluorimetric assay for pyruvate Anal. Biochem. 396 2010 146 151
21. M. Zhou, Z. Diwu, N. Panchuk-Voloshina, and R.P. Haugland A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: applications in detecting the activity of phagocyte NADPH oxidase and other oxidases Anal. Biochem. 253 1997 162 168 (Pubitemid 27508167)