The phage integrase vector pIPI03 allows RecA -independent, site-specific labelling of Staphylococcus lugdunensis strains

Plasmid

Volumn 70, Issue 3, 2013, Pages 377-384

  Heilbronner, Simon ^a   Monk, Ian R ^a   Foster, Timothy J ^a  

^a TRINITY COLLEGE DUBLIN   (Ireland)

Author keywords

Bacteriophage; Plasmid vector; RecA mutant; Staphylococcus lugdunensis

Indexed keywords

INTEGRASE; PHAGE INTEGRASE VECTOR PIPI03; PLASMID VECTOR; RECA PROTEIN; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIOPHAGE; CCRB GENE; CHROMOSOMAL INTEGRATION; CONTROLLED STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; STAPHYLOCOCCUS LUGDUNENSIS; TEMPERATURE SENSITIVE MUTANT;

STAPHYLOCOCCUS; STAPHYLOCOCCUS LUGDUNENSIS;

BACTERIOPHAGE; PLASMID VECTOR; RECA MUTANT; STAPHYLOCOCCUS LUGDUNENSIS;

BACTERIOPHAGES; BASE SEQUENCE; CHROMOSOMES, BACTERIAL; DRUG RESISTANCE, BACTERIAL; GENETIC ENGINEERING; GENETIC VECTORS; INTEGRASES; ISOPROPYL THIOGALACTOSIDE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PLASMIDS; STAPHYLOCOCCUS LUGDUNENSIS; VIRAL PROTEINS;

EID: 84887626153     PISSN: 0147619X     EISSN: 10959890     Source Type: Journal    
DOI: 10.1016/j.plasmid.2013.09.001     Document Type: Article

References (18)

1. Anguera I., et al. Staphylococcus lugdunensis infective endocarditis: description of 10 cases and analysis of native valve, prosthetic valve, and pacemaker lead endocarditis clinical profiles. Heart 2005, 91:e10.
2. Bieber L., Kahlmeter G. Staphylococcus lugdunensis in several niches of the normal skin flora. Clin. Microbiol. Infect. 2010, 16:385-388.
3. Chassaîn B., et al. Multilocus sequence typing analysis of Staphylococcus lugdunensis implies a clonal population structure. J. Clin. Microbiol. 2012, 50:3003-3009.
4. Freney J., et al. Staphylococcus lugdunensis sp. nov. and Staphylococcus schleiferi sp. nov., two species from human clinical specimens. Int. J. Syst. Bacteriol. 1988, 38:168-172.
5. Heilbronner S., et al. Genome sequence of Staphylococcus lugdunensis N920143 allows identification of putative colonization and virulence factors. FEMS Microbiol. Lett. 2011, 322:60-67.
6. Heilbronner, S., et al., 2013. Sortase A promotes virulence in experimental Staphylococcus lugdunensis endocarditis. Micobiology. (accepted for publication).
7. Hirano N., et al. Site-specific recombinases as tools for heterologous gene integration. Appl. Microbiol. Biotechnol. 2011, 92:227-239.
8. Lei M.G., et al. A single copy integration vector that integrates at an engineered site on the Staphylococcus aureus chromosome. BMC Res Notes. 2012, 5:5.
9. Li M.Z., Elledge S.J. Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. Nat. Methods 2007, 4:251-256.
10. Li M.Z., Elledge S.J. SLIC: a method for sequence- and ligation-independent cloning. Methods Mol. Biol. 2012, 852:51-59.
11. Monk I.R., et al. Development of multiple strain competitive index assays for Listeria monocytogenes using pIMC; a new site-specific integrative vector. BMC Microbiol. 2008, 8:96.
12. Monk I.R., et al. Tools for functional postgenomic analysis of Listeria monocytogenes. Appl. Environ. Microbiol. 2008, 74:3921-3934.
13. Monk I.R., et al. Transforming the untransformable: application of direct transformation to manipulate genetically Staphylococcus aureus and Staphylococcus epidermidis. MBio. 2012, 3(2):e00277-11. 10.1128/mBio.00277-11.
14. Patil R., et al. Staphylococcus lugdunensis native tricuspid valve endocarditis: a case report and review of literature. J. Gen. Intern. Med. 2011, 26:1209-1211.
15. Petersen K.V., et al. Repetitive marker-free site-specific integration - a novel tool for multiple chromosomal integration of DNA. Appl. Environ. Microbiol. 2013, 79:3563-3569.
16. Tse H., et al. Complete genome sequence of Staphylococcus lugdunensis strain HKU09-01. J. Bacteriol. 2010, 192:1471-1472.
17. Vandenesch F., et al. Endocarditis due to Staphylococcus lugdunensis: report of 11 cases and review. Clin. Infect. Dis. 1993, 17:871-876.
18. Zapotoczna M., et al. Iron-regulated surface determinant (Isd) proteins of Staphylococcus lugdunensis. J. Bacteriol. 2012, 194:6453-6467.